Restriction map for pF1KB6448
 (Linear) (Six-base) MAPSORT of: KIBB6448.seq  Check: 357  from: 1  to: 1047



KIBB6448.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     597    1047 
   Size:        597     450
 
AleI CACnn'nnGTG

Cuts at:      0     704    1047 
   Size:        704     343
 
ApaI G_GGCC'C

Cuts at:      0    1013    1047 
   Size:       1013      34
 
ApoI r'AATT_y

Cuts at:      0     370    1047 
   Size:        370     677
 
AseI AT'TA_AT

Cuts at:      0     509    1047 
   Size:        509     538
 
AvaI C'yCGr_G

Cuts at:      0      47     149    1047 
   Size:         47     102     898

  Fragments arranged by size: 

                 898     102      47
 
BanII G_rGCy'C

Cuts at:      0      47      66     900    1013    1047 
   Size:         47      19     834     113      34

  Fragments arranged by size: 

                 834     113      47      34      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     221    1047 
   Size:        221     826
 
BclI T'GATC_A

Cuts at:      0    1028    1047 
   Size:       1028      19
 
BfrBI ATG'CAT

Cuts at:      0     647    1047 
   Size:        647     400
 
Bme1580I G_kGCm'C

Cuts at:      0    1013    1047 
   Size:       1013      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      84    1047 
   Size:         84     963
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59      80    1047 
   Size:         59      21     967

  Fragments arranged by size: 

                 967      59      21
 
BsaBI GATnn'nnATC

Cuts at:      0     868    1047 
   Size:        868     179
 
BsaWI w'CCGG_w

Cuts at:      0     660    1047 
   Size:        660     387
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     172     190     428    1047 
   Size:         67     105      18     238     619

  Fragments arranged by size: 

                 619     238     105      67      18
 
BseYI C'CCAG_C

Cuts at:      0     533    1047 
   Size:        533     514
 
Bsp1286I G_dGCh'C

Cuts at:      0      47      66     900    1013    1047 
   Size:         47      19     834     113      34

  Fragments arranged by size: 

                 834     113      47      34      19
 
BspEI T'CCGG_A

Cuts at:      0     660    1047 
   Size:        660     387
 
BspHI T'CATG_A

Cuts at:      0     375    1047 
   Size:        375     672
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     248     563     597    1047 
   Size:        248     315      34     450

  Fragments arranged by size: 

                 450     315     248      34
 
BsrDI GCAATG_nn'

Cuts at:      0     264    1047 
   Size:        264     783
 
BsrGI T'GTAC_A

Cuts at:      0     275    1047 
   Size:        275     772
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     827    1047 
   Size:        827     220
 
EaeI y'GGCC_r

Cuts at:      0     323     947    1047 
   Size:        323     624     100

  Fragments arranged by size: 

                 624     323     100
 
EarI CTCTTCn'nnn_

Cuts at:      0     416    1047 
   Size:        416     631
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31    1047 
   Size:         31    1016
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59      80    1047 
   Size:         59      21     967

  Fragments arranged by size: 

                 967      59      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     228    1009    1010    1047 
   Size:        228     781       1      37

  Fragments arranged by size: 

                 781     228      37       1
 
FspI TGC'GCA

Cuts at:      0      97    1047 
   Size:         97     950
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     417     449     576     608    1047 
   Size:        417      32     127      32     439

  Fragments arranged by size: 

                 439     417     127      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     377    1047 
   Size:        377     670
 
MscI TGG'CCA

Cuts at:      0     325     949    1047 
   Size:        325     624      98

  Fragments arranged by size: 

                 624     325      98
 
MslI CAynn'nnrTG

Cuts at:      0     568     599     704     820     874    1047 
   Size:        568      31     105     116      54     173

  Fragments arranged by size: 

                 568     173     116     105      54      31
 
MspA1I CmG'CkG

Cuts at:      0      13    1047 
   Size:         13    1034
 
NsiI A_TGCA'T

Cuts at:      0     649    1047 
   Size:        649     398
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     771    1047 
   Size:        771     276
 
PpuMI rG'GwC_Cy

Cuts at:      0     228    1047 
   Size:        228     819
 
PspOMI G'GGCC_C

Cuts at:      0    1009    1047 
   Size:       1009      38
 
PstI C_TGCA'G

Cuts at:      0     398     440    1047 
   Size:        398      42     607

  Fragments arranged by size: 

                 607     398      42
 
SfcI C'TryA_G

Cuts at:      0     394     436    1047 
   Size:        394      42     611

  Fragments arranged by size: 

                 611     394      42
 
SmaI CCC'GGG

Cuts at:      0      49     151    1047 
   Size:         49     102     896

  Fragments arranged by size: 

                 896     102      49
 
SspI AAT'ATT

Cuts at:      0     252    1047 
   Size:        252     795
 
StuI AGG'CCT

Cuts at:      0     985    1047 
   Size:        985      62
 
StyI C'CwwG_G

Cuts at:      0     986    1047 
   Size:        986      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     208     304     716    1047 
   Size:        208      96     412     331

  Fragments arranged by size: 

                 412     331     208      96
 
TatI w'GTAC_w

Cuts at:      0     275     302     824    1047 
   Size:        275      27     522     223

  Fragments arranged by size: 

                 522     275     223      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     772     827    1047 
   Size:        772      55     220

  Fragments arranged by size: 

                 772     220      55
 
XmaI C'CCGG_G

Cuts at:      0      47     149    1047 
   Size:         47     102     898

  Fragments arranged by size: 

                 898     102      47
 

 Enzymes that do cut:

     AarI     AleI     ApaI     ApoI     AseI     AvaI    BanII    BciVI
     BclI    BfrBI Bme1580I     BmrI     BpmI    BsaBI    BsaWI    BseRI
    BseYI Bsp1286I    BspEI    BspHI    BspMI    BsrDI    BsrGI    BstXI
     EaeI     EarI     EciI  Eco57MI EcoO109I     FspI    Hin4I     MmeI
     MscI     MslI   MspA1I     NsiI    PflMI    PpuMI   PspOMI     PstI
     SfcI     SmaI     SspI     StuI     StyI    TaqII     TatI     XcmI
     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmnI     ZraI
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