Restriction map for pF1KB6387
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
DsaI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MroI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         780  GACGTC               GACGT^C
AcyI          780  GACGTC               G[GA]^CG[CT]C
AfaI           67  GTAC                 GT^AC
AvaI           87  CCCGGG               C^[CT]CG[GA]G
BalI          492  TGGCCA               TGG^CCA
BanII         268  GAGCCC               G[GA]GC[CT]^C
BsaHI         780  GACGTC               G[GA]^CG[CT]C
BsaI          341  GAGACC               GGTCTC(1/5)
BseRI          94  GAGGAG               GAGGAG(10/8)
BsmAI         341  GAGAC                GTCTC(1/5)
BsmFI         224  GGGAC                GGGAC(10/14)
BsmI          165  GAATGC               GAATGC(1/-1)
BsoBI          87  CCCGGG               C^[CT]CG[GA]G
BstEII        237  GGTCACC              G^GT[ACGT]ACC
BstNI         133  CCTGG                CC^[AT]GG
BstPI         237  GGTCACC              G^GT[ACGT]ACC
CpoI          201  CGGTCCG              CG^G[AT]CCG
DdeI          311  CTCAG                C^T[ACGT]AG
DraI          598  TTTAAA               TTT^AAA
DraII         129  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        240  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          581  GACAGGATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          249  CGGCCG               C^GGCCG
EclXI         249  CGGCCG               C^GGCCG
Eco52I        249  CGGCCG               C^GGCCG
EcoO109I      129  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       237  GGTCACC              G^GT[ACGT]ACC
EcoRII        133  CCTGG                ^CC[AT]GG
HaeII         215  GGCGCT               [GA]GCGC^[CT]
Hin1I         780  GACGTC               G[GA]^CG[CT]C
HindIII       764  AAGCTT               A^AGCTT
MaeII         781  ACGT                 A^CGT
MluNI         492  TGGCCA               TGG^CCA
MscI          492  TGGCCA               TGG^CCA
MvaI          133  CCTGG                CC^[AT]GG
NotI          248  GCGGCCGC             GC^GGCCGC
PflMI         495  CCAATTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         546  CAGCTG               CAG^CTG
RsaI           67  GTAC                 GT^AC
RsrII         201  CGGTCCG              CG^G[AT]CCG
ScaI           66  AGTACT               AGT^ACT
SmaI           87  CCCGGG               CCC^GGG
TfiI           28  GAATC                G^A[AT]TC
Van91I        495  CCAATTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          87  CCCGGG               C^CCGGG
XmaI           87  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI          82  GTGCTC               G[AT]GC[AT]^C
              177  GTGCTC              
AvaII         202  GGTCC                G^G[AT]CC
              225  GGACC               
BfaI           70  CTAG                 C^TAG
              170  CTAG                
BglII         480  AGATCT               A^GATCT
              800  AGATCT              
BsiEI         151  CGACCG               CG[GA][CT]^CG
              249  CGGCCG              
BsiHKAI        82  GTGCTC               G[AT]GC[AT]^C
              177  GTGCTC              
BspMI           9  ACCTGC               ACCTGC(4/8)
              471  GCAGGT              
BssHII         49  GCGCGC               G^CGCGC
               51  GCGCGC              
BstF5I        729  CATCC                GGATG(2/0)
              585  GGATG               
BstXI         345  CCATGTGATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              560  CCAATGGAATGG        
BstYI         480  AGATCT               [GA]^GATC[CT]
              800  AGATCT              
FauI           37  GCGGG                CCCGC(4/6)
              183  GCGGG               
FokI          729  CATCC                GGATG(9/13)
              585  GGATG               
HgaI          121  GACGC                GACGC(5/10)
               13  GCGTC               
HphI           91  GGTGA                GGTGA(8/7)
              239  TCACC               
MaeI           70  CTAG                 C^TAG
              170  CTAG                
MboII         704  GAAGA                GAAGA(8/7)
              803  TCTTC               
MflI          480  AGATCT               [GA]^GATC[CT]
              800  AGATCT              
MspA1I        282  CAGCGG               C[AC]G^C[GT]G
              546  CAGCTG              
PleI          307  GACTC                GAGTC(4/5)
              606  GACTC               
XhoII         480  AGATCT               [GA]^GATC[CT]
              800  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       EaeI        
Fnu4HI      HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeIII      MboI        
MnlI        MseI        MspI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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