Restriction map for pF1KB6387
 (Linear) (Six-base) MAPSORT of: KIBB6387.seq  Check: 3347  from: 1  to: 825



KIBB6387.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AatII G_ACGT'C

Cuts at:      0     784     825 
   Size:        784      41
 
AvaI C'yCGr_G

Cuts at:      0      87     825 
   Size:         87     738
 
BanII G_rGCy'C

Cuts at:      0     272     825 
   Size:        272     553
 
BglII A'GATC_T

Cuts at:      0     480     800     825 
   Size:        480     320      25

  Fragments arranged by size: 

                 480     320      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     309     825 
   Size:        309     516
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     620     700     792     825 
   Size:        395     225      80      92      33

  Fragments arranged by size: 

                 395     225      92      80      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     335     825 
   Size:        335     490
 
BsaHI Gr'CG_yC

Cuts at:      0     781     825 
   Size:        781      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     139     169     332     362     825 
   Size:        139      30     163      30     463

  Fragments arranged by size: 

                 463     163     139      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     825 
   Size:        109     716
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     161     490     825 
   Size:         41     120     329     335

  Fragments arranged by size: 

                 335     329     120      41
 
BsiEI CG_ry'CG

Cuts at:      0     154     252     825 
   Size:        154      98     573

  Fragments arranged by size: 

                 573     154      98
 
BsiHKAI G_wGCw'C

Cuts at:      0      86     181     825 
   Size:         86      95     644

  Fragments arranged by size: 

                 644      95      86
 
BsmI GAATG_Cn'

Cuts at:      0     171     825 
   Size:        171     654
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     181     272     825 
   Size:         86      95      91     553

  Fragments arranged by size: 

                 553      95      91      86
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      18     462     825 
   Size:         18     444     363

  Fragments arranged by size: 

                 444     363      18
 
BssHII G'CGCG_C

Cuts at:      0      49      51     825 
   Size:         49       2     774

  Fragments arranged by size: 

                 774      49       2
 
BstEII G'GTnAC_C

Cuts at:      0     237     825 
   Size:        237     588
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     352     567     825 
   Size:        352     215     258

  Fragments arranged by size: 

                 352     258     215
 
BstYI r'GATC_y

Cuts at:      0     480     800     825 
   Size:        480     320      25

  Fragments arranged by size: 

                 480     320      25
 
BtsI GCAGTG_nn'

Cuts at:      0     149     282     825 
   Size:        149     133     543

  Fragments arranged by size: 

                 543     149     133
 
DraI TTT'AAA

Cuts at:      0     600     825 
   Size:        600     225
 
DraIII CAC_nnn'GTG

Cuts at:      0     245     825 
   Size:        245     580
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     587     825 
   Size:        587     238
 
EaeI y'GGCC_r

Cuts at:      0       2     249     492     825 
   Size:          2     247     243     333

  Fragments arranged by size: 

                 333     247     243       2
 
EagI C'GGCC_G

Cuts at:      0     249     825 
   Size:        249     576
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     620     700     792     825 
   Size:        395     225      80      92      33

  Fragments arranged by size: 

                 395     225      92      80      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     130     825 
   Size:        130     695
 
HaeII r_GCGC'y

Cuts at:      0     219     825 
   Size:        219     606
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     470     502     771     803     825 
   Size:        470      32     269      32      22

  Fragments arranged by size: 

                 470     269      32      32      22
 
HindIII A'AGCT_T

Cuts at:      0     764     825 
   Size:        764      61
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     378     456     825 
   Size:        378      78     369

  Fragments arranged by size: 

                 378     369      78
 
MscI TGG'CCA

Cuts at:      0     494     825 
   Size:        494     331
 
MspA1I CmG'CkG

Cuts at:      0     284     548     825 
   Size:        284     264     277

  Fragments arranged by size: 

                 284     277     264
 
NotI GC'GGCC_GC

Cuts at:      0     249     825 
   Size:        249     576
 
PasI CC'CwG_GG

Cuts at:      0     133     825 
   Size:        133     692
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     501     825 
   Size:        501     324
 
PvuII CAG'CTG

Cuts at:      0     548     825 
   Size:        548     277
 
RsrII CG'GwC_CG

Cuts at:      0     202     825 
   Size:        202     623
 
ScaI AGT'ACT

Cuts at:      0      68     825 
   Size:         68     757
 
SmaI CCC'GGG

Cuts at:      0      89     825 
   Size:         89     736
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     291     559     825 
   Size:        291     268     266

  Fragments arranged by size: 

                 291     268     266
 
TatI w'GTAC_w

Cuts at:      0      66     825 
   Size:         66     759
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364     825 
   Size:        332      32     461

  Fragments arranged by size: 

                 461     332      32
 
XmaI C'CCGG_G

Cuts at:      0      87     825 
   Size:         87     738
 
ZraI GAC'GTC

Cuts at:      0     782     825 
   Size:        782      43
 

 Enzymes that do cut:

    AatII     AvaI    BanII    BglII     BmrI     BpmI     BsaI    BsaHI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
   BssHII   BstEII    BstXI    BstYI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI  Eco57MI EcoO109I    HaeII    Hin4I  HindIII     MmeI
     MscI   MspA1I     NotI     PasI    PflMI    PvuII    RsrII     ScaI
     SmaI    TaqII     TatI     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BseYI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI
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