Restriction map for pF1KB6306
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI          576  GACGCC               G[GA]^CG[CT]C
AlwI          616  GATCC                GGATC(4/5)
Aor51HI       215  AGCGCT               AGC^GCT
ApaI          835  GGGCCC               GGGCC^C
AvaII         332  GGTCC                G^G[AT]CC
BanI          731  GGCACC               G^G[CT][GA]CC
BcgI          429  CGACAAGGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          531  TGATCA               T^GATCA
BfaI          223  CTAG                 C^TAG
BsaBI         610  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         576  GACGCC               G[GA]^CG[CT]C
BsaI          800  GAGACC               GGTCTC(1/5)
BseRI         476  CTCCTC               GAGGAG(10/8)
BspMI         241  ACCTGC               ACCTGC(4/8)
BsrDI         266  GCAATG               GCAATG(2/0)
BssSI         184  CACGAG               CACGAG(-5/-1)
BstYI         615  AGATCC               [GA]^GATC[CT]
DraIII        509  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          538  CCATGG               C^C[GA][CT]GG
Eco47III      215  AGCGCT               AGC^GCT
EcoT14I       538  CCATGG               C^C[AT][AT]GG
FbaI          531  TGATCA               T^GATCA
Hin1I         576  GACGCC               G[GA]^CG[CT]C
MaeI          223  CTAG                 C^TAG
MaeIII        520  GTGAC                ^GT[ACGT]AC
MamI          610  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          615  AGATCC               [GA]^GATC[CT]
MslI          653  CACCTAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          538  CCATGG               C^CATGG
PaeR7I        150  CTCGAG               C^TCGAG
PpuMI         331  GGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         666  CAGCTG               CAG^CTG
SmaI          605  CCCGGG               CCC^GGG
SmlI          150  CTCGAG               C^T[CT][GA]AG
StyI          538  CCATGG               C^C[AT][AT]GG
Tsp45I        520  GTGAC                ^GT[GC]AC
XhoI          150  CTCGAG               C^TCGAG
XhoII         615  AGATCC               [GA]^GATC[CT]
XmaCI         605  CCCGGG               C^CCGGG
XmaI          605  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AvaI          150  CTCGAG               C^[CT]CG[GA]G
              605  CCCGGG              
BanII         408  GGGCTC               G[GA]GC[CT]^C
              835  GGGCCC              
BbsI          169  GAAGAC               GAAGAC(2/6)
              718  GAAGAC              
BpuAI         169  GAAGAC               GAAGAC(2/6)
              718  GAAGAC              
BsaWI         565  ACCGGA               [AT]^CCGG[AT]
              792  ACCGGA              
BsgI          384  GTGCAG               GTGCAG(16/14)
              660  GTGCAG              
BsmAI         316  GAGAC                GTCTC(1/5)
              800  GAGAC               
BsmFI          36  GTCCC                GGGAC(10/14)
              333  GTCCC               
BsoBI         150  CTCGAG               C^[CT]CG[GA]G
              605  CCCGGG              
BsrI          405  ACTGG                ACTGG(1/-1)
              728  ACTGG               
BstAPI        266  GCAATGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              662  GCAGCAGCTGC         
DdeI          122  CTGAG                C^T[ACGT]AG
              656  CTAAG               
DpnII         532  GATC                 ^GATC
              616  GATC                
DraII         331  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              834  AGGGCCC             
EcoO109I      331  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              834  AGGGCCC             
FauI          488  CCCGC                CCCGC(4/6)
              755  CCCGC               
HaeII         215  AGCGCT               [GA]GCGC^[CT]
              674  AGCGCC              
HphI          239  TCACC                GGTGA(8/7)
              599  TCACC               
MboI          532  GATC                 ^GATC
              616  GATC                
NdeII         532  GATC                 ^GATC
              616  GATC                
PstI          279  CTGCAG               CTGCA^G
              492  CTGCAG              
Sau3AI        532  GATC                 ^GATC
              616  GATC                
SfaNI         569  GATGC                GCATC(5/9)
              543  GCATC               
TaqI          151  TCGA                 T^CGA
              546  TCGA                
TthHB8I       151  TCGA                 T^CGA
              546  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
BbvI        BcnI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       ItaI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
RsaI        Sau96I      ScrFI       SfcI        TseI        
TspRI       
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