Restriction map for pF1KB6306
 (Linear) (Six-base) MAPSORT of: KIBB6306.seq  Check: 4777  from: 1  to: 840



KIBB6306.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     250     840 
   Size:        250     590
 
AfeI AGC'GCT

Cuts at:      0     217     840 
   Size:        217     623
 
AleI CACnn'nnGTG

Cuts at:      0     657     840 
   Size:        657     183
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     675     840 
   Size:        675     165
 
AlwNI CAG_nnn'CTG

Cuts at:      0     392     405     668     840 
   Size:        392      13     263     172

  Fragments arranged by size: 

                 392     263     172      13
 
ApaI G_GGCC'C

Cuts at:      0     839     840 
   Size:        839       1
 
AvaI C'yCGr_G

Cuts at:      0     150     605     840 
   Size:        150     455     235

  Fragments arranged by size: 

                 455     235     150
 
BanI G'GyrC_C

Cuts at:      0     731     840 
   Size:        731     109
 
BanII G_rGCy'C

Cuts at:      0     412     839     840 
   Size:        412     427       1

  Fragments arranged by size: 

                 427     412       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     176     725     840 
   Size:        176     549     115

  Fragments arranged by size: 

                 549     176     115
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     418     452     840 
   Size:        418      34     388

  Fragments arranged by size: 

                 418     388      34
 
BclI T'GATC_A

Cuts at:      0     531     840 
   Size:        531     309
 
Bme1580I G_kGCm'C

Cuts at:      0      89     428     455     839     840 
   Size:         89     339      27     384       1

  Fragments arranged by size: 

                 384     339      89      27       1
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     415     840 
   Size:        415     425
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     794     840 
   Size:        794      46
 
BsaBI GATnn'nnATC

Cuts at:      0     614     840 
   Size:        614     226
 
BsaHI Gr'CG_yC

Cuts at:      0     577     840 
   Size:        577     263
 
BsaWI w'CCGG_w

Cuts at:      0     565     792     840 
   Size:        565     227      48

  Fragments arranged by size: 

                 565     227      48
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     730     760     791     821     840 
   Size:        730      30      31      30      19

  Fragments arranged by size: 

                 730      31      30      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     467     840 
   Size:        467     373
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     405     681     840 
   Size:        405     276     159

  Fragments arranged by size: 

                 405     276     159
 
BsiHKAI G_wGCw'C

Cuts at:      0     304     374     441     812     840 
   Size:        304      70      67     371      28

  Fragments arranged by size: 

                 371     304      70      67      28
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     304     374     412     428     441     455     812 
   Size:         89     215      70      38      16      13      14     357

Cuts at:    812     839     840 
   Size:         27       1

  Fragments arranged by size: 

                 357     215      89      70      38      27      16      14
                  13       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     250     840 
   Size:        250     590
 
BsrDI GCAATG_nn'

Cuts at:      0     273     840 
   Size:        273     567
 
BssSI C'ACGA_G

Cuts at:      0     184     840 
   Size:        184     656
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     272     668     840 
   Size:        272     396     172

  Fragments arranged by size: 

                 396     272     172
 
BstYI r'GATC_y

Cuts at:      0     615     840 
   Size:        615     225
 
BtgI C'CryG_G

Cuts at:      0     538     840 
   Size:        538     302
 
BtsI GCAGTG_nn'

Cuts at:      0     288     304     372     393     840 
   Size:        288      16      68      21     447

  Fragments arranged by size: 

                 447     288      68      21      16
 
DraIII CAC_nnn'GTG

Cuts at:      0     514     840 
   Size:        514     326
 
EcoO109I rG'GnC_Cy

Cuts at:      0     332     835     840 
   Size:        332     503       5

  Fragments arranged by size: 

                 503     332       5
 
HaeII r_GCGC'y

Cuts at:      0     219     678     840 
   Size:        219     459     162

  Fragments arranged by size: 

                 459     219     162
 
MslI CAynn'nnrTG

Cuts at:      0     657     840 
   Size:        657     183
 
MspA1I CmG'CkG

Cuts at:      0      71     200     209     491     668     758     840 
   Size:         71     129       9     282     177      90      82

  Fragments arranged by size: 

                 282     177     129      90      82      71       9
 
NcoI C'CATG_G

Cuts at:      0     538     840 
   Size:        538     302
 
PfoI T'CCnGG_A

Cuts at:      0     334     840 
   Size:        334     506
 
PpuMI rG'GwC_Cy

Cuts at:      0     332     840 
   Size:        332     508
 
PspOMI G'GGCC_C

Cuts at:      0     835     840 
   Size:        835       5
 
PspXI vC'TCGA_Gb

Cuts at:      0     150     840 
   Size:        150     690
 
PstI C_TGCA'G

Cuts at:      0     283     496     840 
   Size:        283     213     344

  Fragments arranged by size: 

                 344     283     213
 
PvuII CAG'CTG

Cuts at:      0     668     840 
   Size:        668     172
 
SanDI GG'GwC_CC

Cuts at:      0     332     840 
   Size:        332     508
 
SfcI C'TryA_G

Cuts at:      0     279     492     822     840 
   Size:        279     213     330      18

  Fragments arranged by size: 

                 330     279     213      18
 
SmaI CCC'GGG

Cuts at:      0     607     840 
   Size:        607     233
 
SmlI C'TyrA_G

Cuts at:      0     150     840 
   Size:        150     690
 
StyI C'CwwG_G

Cuts at:      0     538     840 
   Size:        538     302
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     737     840 
   Size:        737     103
 
TatI w'GTAC_w

Cuts at:      0      53     840 
   Size:         53     787
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     791     823     840 
   Size:        791      32      17

  Fragments arranged by size: 

                 791      32      17
 
XhoI C'TCGA_G

Cuts at:      0     150     840 
   Size:        150     690
 
XmaI C'CCGG_G

Cuts at:      0     605     840 
   Size:        605     235
 

 Enzymes that do cut:

     AarI     AfeI     AleI     AlfI    AlwNI     ApaI     AvaI     BanI
    BanII     BbsI     BcgI     BclI Bme1580I     BmrI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BssSI   BstAPI    BstYI     BtgI     BtsI   DraIII EcoO109I
    HaeII     MslI   MspA1I     NcoI     PfoI    PpuMI   PspOMI    PspXI
     PstI    PvuII    SanDI     SfcI     SmaI     SmlI     StyI    TaqII
     TatI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XcmI     XmnI     ZraI
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