Restriction map for pF1KB0960
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        EclXI       Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MroI        
MslI        MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tth111I     Van91I      
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          967  GTCTAC               GT^[AC][GT]AC
AcsI          645  AAATTC               [GA]^AATT[CT]
AcyI          266  GGCGCC               G[GA]^CG[CT]C
AhdI          847  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI           48  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       161  AGCGCT               AGC^GCT
ApoI          645  AAATTC               [GA]^AATT[CT]
AspEI         847  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          389  TGGCCA               TGG^CCA
BbeI          266  GGCGCC               GGCGC^C
BbsI          716  GTCTTC               GAAGAC(2/6)
BciVI         991  GGATAC               GTATCC(6/5)
BlpI           94  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       94  GCTGAGC              GC^T[ACGT]AGC
BpuAI         716  GTCTTC               GAAGAC(2/6)
BsaHI         266  GGCGCC               G[GA]^CG[CT]C
BspMI         257  ACCTGC               ACCTGC(4/8)
BsrBI          97  GAGCGG               CCGCTC(-3/-3)
BstXI         222  CCAGGGTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I         48  CCTCAGG              CC^T[ACGT]AGG
CelII          94  GCTGAGC              GC^T[ACGT]AGC
EaeI          389  TGGCCA               [CT]^GGCC[GA]
Eam1105I      847  GACAGGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      161  AGCGCT               AGC^GCT
Eco81I         48  CCTCAGG              CC^T[ACGT]AGG
Hin1I         266  GGCGCC               G[GA]^CG[CT]C
KasI          266  GGCGCC               G^GCGCC
MluNI         389  TGGCCA               TGG^CCA
MscI          389  TGGCCA               TGG^CCA
MseI          460  TTAA                 T^TAA
MspA1I        121  CAGCGG               C[AC]G^C[GT]G
NarI          266  GGCGCC               GG^CGCC
PaeR7I        579  CTCGAG               C^TCGAG
PstI         1010  CTGCAG               CTGCA^G
SfcI         1010  CTGCAG               C^T[GA][CT]AG
SfoI          266  GGCGCC               GGC^GCC
SphI         1095  GCATGC               GCATG^C
TfiI           18  GAATC                G^A[AT]TC
Tru9I         460  TTAA                 T^TAA
XcmI          222  CCAGGGTCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          579  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          219  GTAC                 GT^AC
              255  GTAC                
AspHI         610  GAGCAC               G[AT]GC[AT]^C
              822  GAGCAC              
BanII         143  GAGCCC               G[GA]GC[CT]^C
              945  GAGCCC              
BfaI          941  CTAG                 C^TAG
             1126  CTAG                
BsaI           11  GAGACC               GGTCTC(1/5)
              854  GGTCTC              
BsgI          901  CTGCAC               GTGCAG(16/14)
              617  GTGCAG              
BsiHKAI       610  GAGCAC               G[AT]GC[AT]^C
              822  GAGCAC              
BsrI          212  ACTGG                ACTGG(1/-1)
              469  CCAGT               
BstYI         398  AGATCC               [GA]^GATC[CT]
              775  AGATCC              
CfoI          162  GCGC                 GCG^C
              267  GCGC                
FauI           39  CCCGC                CCCGC(4/6)
              123  GCGGG               
HaeII         161  AGCGCT               [GA]GCGC^[CT]
              266  GGCGCC              
HhaI          162  GCGC                 GCG^C
              267  GCGC                
HinP1I        162  GCGC                 G^CGC
              267  GCGC                
MaeI          941  CTAG                 C^TAG
             1126  CTAG                
MflI          398  AGATCC               [GA]^GATC[CT]
              775  AGATCC              
PleI         1138  GACTC                GAGTC(4/5)
              507  GAGTC               
RsaI          219  GTAC                 GT^AC
              255  GTAC                
SfaNI         156  GATGC                GCATC(5/9)
             1183  GCATC               
SmlI          579  CTCGAG               C^T[CT][GA]AG
             1087  CTCAAG              
TaqI          580  TCGA                 T^CGA
              858  TCGA                
Tsp45I        248  GTGAC                ^GT[GC]AC
              876  GTCAC               
TspRI         470  CAGTG                CA[GC]TG[ACGT][ACGT]^
              615  CAGTG               
TthHB8I       580  TCGA                 T^CGA
              858  TCGA                
XhoII         398  AGATCC               [GA]^GATC[CT]
              775  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BanI        BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PpuMI       SapI        Sau3AI      Sau96I      ScrFI       
SmaI        TseI        Tsp509I     XmaCI       XmaI        
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