Restriction map for pF1KB0960
 (Linear) (Six-base) MAPSORT of: KIBB0960.seq  Check: 1386  from: 1  to: 1275



KIBB0960.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AccI GT'mk_AC

Cuts at:      0     968    1275 
   Size:        968     307
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     103     703    1275 
   Size:         43      60     600     572

  Fragments arranged by size: 

                 600     572      60      43
 
AfeI AGC'GCT

Cuts at:      0     163    1275 
   Size:        163    1112
 
AhdI GACnn_n'nnGTC

Cuts at:      0     852    1275 
   Size:        852     423
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     182    1275 
   Size:        182    1093
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1138    1170    1275 
   Size:       1138      32     105

  Fragments arranged by size: 

                1138     105      32
 
ApoI r'AATT_y

Cuts at:      0     645    1275 
   Size:        645     630
 
AvaI C'yCGr_G

Cuts at:      0     146     579     842    1142    1275 
   Size:        146     433     263     300     133

  Fragments arranged by size: 

                 433     300     263     146     133
 
BanI G'GyrC_C

Cuts at:      0      35     266     367    1275 
   Size:         35     231     101     908

  Fragments arranged by size: 

                 908     231     101      35
 
BanII G_rGCy'C

Cuts at:      0     147     949    1275 
   Size:        147     802     326

  Fragments arranged by size: 

                 802     326     147
 
BbeI G_GCGC'C

Cuts at:      0     270    1275 
   Size:        270    1005
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     709    1275 
   Size:        709     566
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     985    1275 
   Size:        985     290
 
BlpI GC'TnA_GC

Cuts at:      0      95    1275 
   Size:         95    1180
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1195    1219    1273    1275 
   Size:       1195      24      54       2

  Fragments arranged by size: 

                1195      54      24       2
 
Bpu10I CC'TnA_GC

Cuts at:      0    1100    1275 
   Size:       1100     175
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1072    1275 
   Size:       1072     203
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       5     860    1275 
   Size:          5     855     415

  Fragments arranged by size: 

                 855     415       5
 
BsaHI Gr'CG_yC

Cuts at:      0     267    1275 
   Size:        267    1008
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      52      82    1275 
   Size:         52      30    1193

  Fragments arranged by size: 

                1193      52      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     316     451     454     777    1081    1084    1275 
   Size:        127     189     135       3     323     304       3     191

  Fragments arranged by size: 

                 323     304     191     189     135     127       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     261     892    1275 
   Size:        261     631     383

  Fragments arranged by size: 

                 631     383     261
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     638     886    1275 
   Size:        638     248     389

  Fragments arranged by size: 

                 638     389     248
 
BsiHKAI G_wGCw'C

Cuts at:      0     614     826    1275 
   Size:        614     212     449

  Fragments arranged by size: 

                 614     449     212
 
Bsp1286I G_dGCh'C

Cuts at:      0     147     614     826     949    1275 
   Size:        147     467     212     123     326

  Fragments arranged by size: 

                 467     326     212     147     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266    1275 
   Size:        266    1009
 
BsrBI CCG'CTC

Cuts at:      0      99    1275 
   Size:         99    1176
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     229    1275 
   Size:        229    1046
 
BstYI r'GATC_y

Cuts at:      0     398     775    1275 
   Size:        398     377     500

  Fragments arranged by size: 

                 500     398     377
 
Bsu36I CC'TnA_GG

Cuts at:      0      49    1275 
   Size:         49    1226
 
EaeI y'GGCC_r

Cuts at:      0     389    1275 
   Size:        389     886
 
EarI CTCTTCn'nnn_

Cuts at:      0      22     131     146     327     421     814    1275 
   Size:         22     109      15     181      94     393     461

  Fragments arranged by size: 

                 461     393     181     109      94      22      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      23    1275 
   Size:         23    1252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     103     703    1195    1219    1273    1275 
   Size:         43      60     600     492      24      54       2

  Fragments arranged by size: 

                 600     492      60      54      43      24       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     226     408     724     839    1275 
   Size:        226     182     316     115     436

  Fragments arranged by size: 

                 436     316     226     182     115
 
HaeII r_GCGC'y

Cuts at:      0     165     270    1275 
   Size:        165     105    1005

  Fragments arranged by size: 

                1005     165     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     573     605     838     870    1067    1099    1168    1200 
   Size:        573      32     233      32     197      32      69      32

Cuts at:   1200    1275 
   Size:         75

  Fragments arranged by size: 

                 573     233     197      75      69      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     266    1275 
   Size:        266    1009
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     188    1275 
   Size:        188    1087
 
MscI TGG'CCA

Cuts at:      0     391    1275 
   Size:        391     884
 
MspA1I CmG'CkG

Cuts at:      0     123    1275 
   Size:        123    1152
 
NarI GG'CG_CC

Cuts at:      0     267    1275 
   Size:        267    1008
 
NspI r_CATG'y

Cuts at:      0     811     985    1099    1275 
   Size:        811     174     114     176

  Fragments arranged by size: 

                 811     176     174     114
 
PasI CC'CwG_GG

Cuts at:      0     864    1275 
   Size:        864     411
 
PfoI T'CCnGG_A

Cuts at:      0     401     987    1275 
   Size:        401     586     288

  Fragments arranged by size: 

                 586     401     288
 
PpuMI rG'GwC_Cy

Cuts at:      0     226     408     724     839    1275 
   Size:        226     182     316     115     436

  Fragments arranged by size: 

                 436     316     226     182     115
 
PspXI vC'TCGA_Gb

Cuts at:      0     579    1275 
   Size:        579     696
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     576     608    1275 
   Size:        576      32     667

  Fragments arranged by size: 

                 667     576      32
 
PstI C_TGCA'G

Cuts at:      0    1014    1275 
   Size:       1014     261
 
SanDI GG'GwC_CC

Cuts at:      0     839    1275 
   Size:        839     436
 
SapI GCTCTTCn'nnn_

Cuts at:      0      22     327     814    1275 
   Size:         22     305     487     461

  Fragments arranged by size: 

                 487     461     305      22
 
SfcI C'TryA_G

Cuts at:      0    1010    1275 
   Size:       1010     265
 
SfoI GGC'GCC

Cuts at:      0     268    1275 
   Size:        268    1007
 
SmaI CCC'GGG

Cuts at:      0     148     844    1144    1275 
   Size:        148     696     300     131

  Fragments arranged by size: 

                 696     300     148     131
 
SmlI C'TyrA_G

Cuts at:      0     579    1087    1275 
   Size:        579     508     188

  Fragments arranged by size: 

                 579     508     188
 
SphI G_CATG'C

Cuts at:      0    1099    1275 
   Size:       1099     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      52      84    1275 
   Size:         52      32    1191

  Fragments arranged by size: 

                1191      52      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     229    1275 
   Size:        229    1046
 
XhoI C'TCGA_G

Cuts at:      0     579    1275 
   Size:        579     696
 
XmaI C'CCGG_G

Cuts at:      0     146     842    1142    1275 
   Size:        146     696     300     133

  Fragments arranged by size: 

                 696     300     146     133
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AhdI     AlfI     AloI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BciVI     BlpI     BpmI   Bpu10I
    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BstXI    BstYI   Bsu36I     EaeI     EarI
     EciI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MmeI     MscI
   MspA1I     NarI     NspI     PasI     PfoI    PpuMI    PspXI     PsrI
     PstI    SanDI     SapI     SfcI     SfoI     SmaI     SmlI     SphI
     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AleI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI    BsaAI    BsaBI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com