Restriction map for pF1KB8146
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspHI       
AspI        AviII       AvrII       BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     Bsu36I      
Cfr10I      ClaI        DraI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoRI       FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MunI        NaeI        NarI        NdeI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp509I     Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII         830  CGCG                 CG^CG
AfaI           14  GTAC                 GT^AC
AhdI         1054  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       478  AGCGCT               AGC^GCT
AspEI        1054  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          122  CCCGGG               C^[CT]CG[GA]G
BalI          863  TGGCCA               TGG^CCA
BamHI         156  GGATCC               G^GATCC
BanI          146  GGCACC               G^G[CT][GA]CC
BcgI          318  CGAAGGTGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          193  CTAG                 C^TAG
BlpI          697  GCTGAGC              GC^T[ACGT]AGC
BmyI          145  GGGCAC               G[AGT]GC[ACT]^C
BpmI          187  CTGGAG               CTGGAG(16/14)
Bpu1102I      697  GCTGAGC              GC^T[ACGT]AGC
BsgI          504  CTGCAC               GTGCAG(16/14)
BsmI          567  GAATGC               GAATGC(1/-1)
BsoBI         122  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      145  GGGCAC               G[AGT]GC[ACT]^C
BspMI          84  ACCTGC               ACCTGC(4/8)
BsrDI         350  GCAATG               GCAATG(2/0)
BstEII        846  GGTAACC              G^GT[ACGT]ACC
BstPI         846  GGTAACC              G^GT[ACGT]ACC
BstUI         830  CGCG                 CG^CG
CelII         697  GCTGAGC              GC^T[ACGT]AGC
CpoI          415  CGGACCG              CG^G[AT]CCG
DraII        1040  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        987  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          680  GACTGAAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          863  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1054  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      478  AGCGCT               AGC^GCT
EcoO109I     1040  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       846  GGTAACC              G^GT[ACGT]ACC
EcoRV         658  GATATC               GAT^ATC
EcoT22I       569  ATGCAT               ATGCA^T
MaeI          193  CTAG                 C^TAG
MluNI         863  TGGCCA               TGG^CCA
MscI          863  TGGCCA               TGG^CCA
MvnI          830  CGCG                 CG^CG
NcoI          842  CCATGG               C^CATGG
NheI          192  GCTAGC               G^CTAGC
NsiI          569  ATGCAT               ATGCA^T
PflMI          64  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1040  AGGTCCC              [GA]G^G[AT]CC[CT]
RsaI           14  GTAC                 GT^AC
RsrII         415  CGGACCG              CG^G[AT]CCG
SmaI          122  CCCGGG               CCC^GGG
SmlI          178  CTCAAG               C^T[CT][GA]AG
Van91I         64  CCAGCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          575  CCAACAGCGGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         122  CCCGGG               C^CCGGG
XmaI          122  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Asp700I       741  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1083  GAAGCCCTTC          
BseRI          93  CTCCTC               GAGGAG(10/8)
              718  GAGGAG              
BsmAI         374  GAGAC                GTCTC(1/5)
              647  GAGAC               
BsmFI         936  GTCCC                GGGAC(10/14)
             1042  GTCCC               
BstXI         370  CCAGGAGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              632  CCATCAATGTGG        
BstYI         156  GGATCC               [GA]^GATC[CT]
              909  GGATCT              
HaeII         132  GGCGCT               [GA]GCGC^[CT]
              478  AGCGCT              
HphI          381  GGTGA                GGTGA(8/7)
             1112  TCACC               
MflI          156  GGATCC               [GA]^GATC[CT]
              909  GGATCT              
MseI          752  TTAA                 T^TAA
              905  TTAA                
MslI          258  CATAAATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              633  CATCAATGTG          
MspA1I        579  CAGCGG               C[AC]G^C[GT]G
             1045  CCGCTG              
NspI          702  GCATGT               [GA]CATG^[CT]
              758  ACATGC              
PleI          284  GACTC                GAGTC(4/5)
              687  GAGTC               
Tru9I         752  TTAA                 T^TAA
              905  TTAA                
XhoII         156  GGATCC               [GA]^GATC[CT]
              909  GGATCT              
XmnI          741  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1083  GAAGCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BbvI        BcnI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     
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