Restriction map for pF1KB8146
 (Linear) (Six-base) MAPSORT of: KIBB8146.seq  Check: 9373  from: 1  to: 1119



KIBB8146.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     457     703    1119 
   Size:        385      72     246     416

  Fragments arranged by size: 

                 416     385     246      72
 
AfeI AGC'GCT

Cuts at:      0     480    1119 
   Size:        480     639
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1059    1119 
   Size:       1059      60
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70     669    1027    1119 
   Size:         70     599     358      92

  Fragments arranged by size: 

                 599     358      92      70
 
AvaI C'yCGr_G

Cuts at:      0     122    1119 
   Size:        122     997
 
BamHI G'GATC_C

Cuts at:      0     156    1119 
   Size:        156     963
 
BanI G'GyrC_C

Cuts at:      0     146    1119 
   Size:        146     973
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     307     341    1119 
   Size:        307      34     778

  Fragments arranged by size: 

                 778     307      34
 
BfrBI ATG'CAT

Cuts at:      0     571    1119 
   Size:        571     548
 
BlpI GC'TnA_GC

Cuts at:      0     698    1119 
   Size:        698     421
 
Bme1580I G_kGCm'C

Cuts at:      0     149    1119 
   Size:        149     970
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     387     933    1119 
   Size:        387     546     186

  Fragments arranged by size: 

                 546     387     186
 
BmtI G_CTAG'C

Cuts at:      0     196    1119 
   Size:        196     923
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1033    1119 
   Size:       1033      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1119 
   Size:        208     911
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163    1119 
   Size:        163     956
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     365     395    1119 
   Size:        365      30     724

  Fragments arranged by size: 

                 724     365      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      84     733    1119 
   Size:         84     649     386

  Fragments arranged by size: 

                 649     386      84
 
BseYI C'CCAG_C

Cuts at:      0      63     764    1119 
   Size:         63     701     355

  Fragments arranged by size: 

                 701     355      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     489    1119 
   Size:        489     630
 
BsmI GAATG_Cn'

Cuts at:      0     573    1119 
   Size:        573     546
 
Bsp1286I G_dGCh'C

Cuts at:      0     149    1119 
   Size:        149     970
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      93    1119 
   Size:         93    1026
 
BsrDI GCAATG_nn'

Cuts at:      0     357    1119 
   Size:        357     762
 
BstEII G'GTnAC_C

Cuts at:      0     846    1119 
   Size:        846     273
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     377     639    1119 
   Size:        377     262     480

  Fragments arranged by size: 

                 480     377     262
 
BstYI r'GATC_y

Cuts at:      0     156     909    1119 
   Size:        156     753     210

  Fragments arranged by size: 

                 753     210     156
 
BtgI C'CryG_G

Cuts at:      0     150     769     842    1119 
   Size:        150     619      73     277

  Fragments arranged by size: 

                 619     277     150      73
 
BtsI GCAGTG_nn'

Cuts at:      0      52    1119 
   Size:         52    1067
 
DraIII CAC_nnn'GTG

Cuts at:      0     992    1119 
   Size:        992     127
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     686    1119 
   Size:        686     433
 
EaeI y'GGCC_r

Cuts at:      0     863    1119 
   Size:        863     256
 
EarI CTCTTCn'nnn_

Cuts at:      0     476     679     710    1119 
   Size:        476     203      31     409

  Fragments arranged by size: 

                 476     409     203      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      99     429     994    1119 
   Size:         99     330     565     125

  Fragments arranged by size: 

                 565     330     125      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     385     457     703    1119 
   Size:        208     177      72     246     416

  Fragments arranged by size: 

                 416     246     208     177      72
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1041    1119 
   Size:       1041      78
 
EcoRV GAT'ATC

Cuts at:      0     660    1119 
   Size:        660     459
 
HaeII r_GCGC'y

Cuts at:      0     136     482    1119 
   Size:        136     346     637

  Fragments arranged by size: 

                 637     346     136
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     200     232     644     676    1045    1077    1119 
   Size:        200      32     412      32     369      32      42

  Fragments arranged by size: 

                 412     369     200      42      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     599    1119 
   Size:        288     311     520

  Fragments arranged by size: 

                 520     311     288
 
MscI TGG'CCA

Cuts at:      0     865    1119 
   Size:        865     254
 
MslI CAynn'nnrTG

Cuts at:      0     262     637    1119 
   Size:        262     375     482

  Fragments arranged by size: 

                 482     375     262
 
MspA1I CmG'CkG

Cuts at:      0     581    1047    1119 
   Size:        581     466      72

  Fragments arranged by size: 

                 581     466      72
 
NcoI C'CATG_G

Cuts at:      0     842    1119 
   Size:        842     277
 
NheI G'CTAG_C

Cuts at:      0     192    1119 
   Size:        192     927
 
NsiI A_TGCA'T

Cuts at:      0     573    1119 
   Size:        573     546
 
NspI r_CATG'y

Cuts at:      0     706     762    1119 
   Size:        706      56     357

  Fragments arranged by size: 

                 706     357      56
 
PasI CC'CwG_GG

Cuts at:      0     489     618    1119 
   Size:        489     129     501

  Fragments arranged by size: 

                 501     489     129
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      70    1119 
   Size:         70    1049
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     412     444    1119 
   Size:        412      32     675

  Fragments arranged by size: 

                 675     412      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1041    1119 
   Size:       1041      78
 
PstI C_TGCA'G

Cuts at:      0      13     545    1052    1119 
   Size:         13     532     507      67

  Fragments arranged by size: 

                 532     507      67      13
 
RsrII CG'GwC_CG

Cuts at:      0     416    1119 
   Size:        416     703
 
SfcI C'TryA_G

Cuts at:      0       9     513     541    1048    1119 
   Size:          9     504      28     507      71

  Fragments arranged by size: 

                 507     504      71      28       9
 
SmaI CCC'GGG

Cuts at:      0     124    1119 
   Size:        124     995
 
SmlI C'TyrA_G

Cuts at:      0     178    1119 
   Size:        178     941
 
StyI C'CwwG_G

Cuts at:      0     164     270     842    1067    1119 
   Size:        164     106     572     225      52

  Fragments arranged by size: 

                 572     225     164     106      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     369     433     481    1119 
   Size:        369      64      48     638

  Fragments arranged by size: 

                 638     369      64      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     306     338     365     397    1119 
   Size:        306      32      27      32     722

  Fragments arranged by size: 

                 722     306      32      32      27
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     582    1119 
   Size:        582     537
 
XmaI C'CCGG_G

Cuts at:      0     122    1119 
   Size:        122     997
 
XmnI GAAnn'nnTTC

Cuts at:      0     745    1087    1119 
   Size:        745     342      32

  Fragments arranged by size: 

                 745     342      32
 

 Enzymes that do cut:

     AcuI     AfeI     AhdI    AlwNI     AvaI    BamHI     BanI     BcgI
    BfrBI     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI
    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BspMI    BsrDI
   BstEII    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI
     EarI     EciI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NcoI     NheI     NsiI     NspI     PasI
    PflMI     PpiI    PpuMI     PstI    RsrII     SfcI     SmaI     SmlI
     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI     BglI    BglII    BmgBI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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