Restriction map for pF1KB8364
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BamHI       BanI        
BbeI        BciVI       BclI        BfaI        BfrI        
BlnI        BsaBI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BsrI        BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HaeII       
HgaI        HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        TspRI       
Tth111I     Van91I      XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        1435  GACGTC               GACGT^C
AcsI          574  GAATTT               [GA]^AATT[CT]
AcyI         1435  GACGTC               G[GA]^CG[CT]C
AlwI          693  GATCC                GGATC(4/5)
ApaI           82  GGGCCC               GGGCC^C
ApoI          574  GAATTT               [GA]^AATT[CT]
AspHI        1269  GAGCTC               G[AT]GC[AT]^C
AvaII         240  GGACC                G^G[AT]CC
AviII         260  TGCGCA               TGC^GCA
BalI         1031  TGGCCA               TGG^CCA
BbrPI        1403  CACGTG               CAC^GTG
BcgI          801  CGAGGATATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          216  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      216  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1403  CACGTG               [CT]AC^GT[GA]
BsaHI        1435  GACGTC               G[GA]^CG[CT]C
BsaI          358  GAGACC               GGTCTC(1/5)
BsaWI         932  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1269  GAGCTC               G[AT]GC[AT]^C
BsmFI         708  GTCCC                GGGAC(10/14)
BspLU11I      413  ACATGT               A^CATGT
BssSI          75  CACGAG               CACGAG(-5/-1)
BstEII         12  GGTGACC              G^GT[ACGT]ACC
BstPI          12  GGTGACC              G^GT[ACGT]ACC
CelII         216  GCTGAGC              GC^T[ACGT]AGC
DrdI           15  GACCCAGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1031  TGGCCA               [CT]^GGCC[GA]
EarI          685  GAAGAG               CTCTTC(1/4)
EcoO65I        12  GGTGACC              G^GT[ACGT]ACC
EcoT14I       740  CCATGG               C^C[AT][AT]GG
FspI          260  TGCGCA               TGC^GCA
Hin1I        1435  GACGTC               G[GA]^CG[CT]C
HincII        202  GTCAAC               GT[CT]^[GA]AC
HindII        202  GTCAAC               GT[CT]^[GA]AC
Ksp632I       685  GAAGAG               CTCTTC(1/4)
MluNI        1031  TGGCCA               TGG^CCA
MscI         1031  TGGCCA               TGG^CCA
MseI         1037  TTAA                 T^TAA
NcoI          740  CCATGG               C^CATGG
PaeR7I        105  CTCGAG               C^TCGAG
PmaCI        1403  CACGTG               CAC^GTG
PmlI         1403  CACGTG               CAC^GTG
SacI         1269  GAGCTC               GAGCT^C
SfiI          967  GGCCTCAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI           50  CCCGGG               CCC^GGG
SmlI          105  CTCGAG               C^T[CT][GA]AG
StyI          740  CCATGG               C^C[AT][AT]GG
Tru9I        1037  TTAA                 T^TAA
Tsp45I         13  GTGAC                ^GT[GC]AC
Tsp509I       575  AATT                 ^AATT
XcmI         1088  CCAAGCAGGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          105  CTCGAG               C^TCGAG
XmaCI          50  CCCGGG               C^CCGGG
XmaI           50  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AflIII        413  ACATGT               A^C[GA][CT]GT
             1402  ACACGT              
AocI          438  CCTTAGG              CC^T[ACGT]AGG
              669  CCTCAGG             
BbsI          387  GAAGAC               GAAGAC(2/6)
             1239  GAAGAC              
BglI          146  GCCTGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              968  GCCTCAAAGGC         
BglII         713  AGATCT               A^GATCT
              917  AGATCT              
BpuAI         387  GAAGAC               GAAGAC(2/6)
             1239  GAAGAC              
BspMI         896  ACCTGC               ACCTGC(4/8)
             1258  GCAGGT              
BsrBI          66  CCGCTC               CCGCTC(-3/-3)
              753  CCGCTC              
BsrDI         777  CATTGC               GCAATG(2/0)
             1002  CATTGC              
BsrFI         944  GCCGGC               [GA]^CCGG[CT]
             1199  GCCGGC              
BstXI         170  CCAGCGGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1373  CCAGGGCCGTGG        
Bsu36I        438  CCTTAGG              CC^T[ACGT]AGG
              669  CCTCAGG             
Cfr10I        944  GCCGGC               [GA]^CCGG[CT]
             1199  GCCGGC              
DraII         965  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1283  GGGGCCT             
DsaI          740  CCATGG               C^C[GA][CT]GG
             1379  CCGTGG              
Eco81I        438  CCTTAGG              CC^T[ACGT]AGG
              669  CCTCAGG             
EcoO109I      965  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1283  GGGGCCT             
HphI           12  GGTGA                GGTGA(8/7)
              188  TCACC               
MaeIII         13  GTGAC                ^GT[ACGT]AC
              196  GTTAC               
NaeI          944  GCCGGC               GCC^GGC
             1199  GCCGGC              
NgoMIV        944  GCCGGC               G^CCGGC
             1199  GCCGGC              
PvuII         448  CAGCTG               CAG^CTG
             1108  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AvaI        BanII       BbvCI       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeII       MboI        MboII       MflI        MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TseI        TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com