Restriction map for pF1KB8364
 (Linear) (Six-base) MAPSORT of: KIBB8364.seq  Check: 174  from: 1  to: 1449



KIBB8364.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1249    1449 
   Size:       1249     200
 
AatII G_ACGT'C

Cuts at:      0    1439    1449 
   Size:       1439      10
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     496    1338    1449 
   Size:         42     454     842     111

  Fragments arranged by size: 

                 842     454     111      42
 
AflIII A'CryG_T

Cuts at:      0     413    1402    1449 
   Size:        413     989      47

  Fragments arranged by size: 

                 989     413      47
 
AleI CACnn'nnGTG

Cuts at:      0     727    1449 
   Size:        727     722
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     400     432    1449 
   Size:        400      32    1017

  Fragments arranged by size: 

                1017     400      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     213     510     873    1128    1449 
   Size:        213     297     363     255     321

  Fragments arranged by size: 

                 363     321     297     255     213
 
ApaI G_GGCC'C

Cuts at:      0      86    1449 
   Size:         86    1363
 
ApoI r'AATT_y

Cuts at:      0     574    1449 
   Size:        574     875
 
AvaI C'yCGr_G

Cuts at:      0      50     105     116    1449 
   Size:         50      55      11    1333

  Fragments arranged by size: 

                1333      55      50      11
 
BanII G_rGCy'C

Cuts at:      0      86     223    1273    1321    1345    1449 
   Size:         86     137    1050      48      24     104

  Fragments arranged by size: 

                1050     137     104      86      48      24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     394    1246    1449 
   Size:        394     852     203

  Fragments arranged by size: 

                 852     394     203
 
BbvCI CC'TCA_GC

Cuts at:      0     782     827    1303    1449 
   Size:        782      45     476     146

  Fragments arranged by size: 

                 782     476     146      45
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     790     824    1449 
   Size:        790      34     625

  Fragments arranged by size: 

                 790     625      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     152     974    1449 
   Size:        152     822     475

  Fragments arranged by size: 

                 822     475     152
 
BglII A'GATC_T

Cuts at:      0     713     917    1449 
   Size:        713     204     532

  Fragments arranged by size: 

                 713     532     204
 
BlpI GC'TnA_GC

Cuts at:      0     217    1449 
   Size:        217    1232
 
Bme1580I G_kGCm'C

Cuts at:      0      86    1449 
   Size:         86    1363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     355     376     472     502     649     934    1012 
   Size:        253     102      21      96      30     147     285      78

Cuts at:   1012    1087    1204    1225    1305    1329    1356    1449 
   Size:         75     117      21      80      24      27      93

  Fragments arranged by size: 

                 285     253     147     117     102      96      93      80
                  78      75      30      27      24      21      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     782     827    1303    1449 
   Size:        782      45     476     146

  Fragments arranged by size: 

                 782     476     146      45
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     352    1449 
   Size:        352    1097
 
BsaAI yAC'GTr

Cuts at:      0    1405    1449 
   Size:       1405      44
 
BsaHI Gr'CG_yC

Cuts at:      0    1436    1449 
   Size:       1436      13
 
BsaWI w'CCGG_w

Cuts at:      0     932    1449 
   Size:        932     517
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     349     379     691     721     739     769    1449 
   Size:        349      30     312      30      18      30     680

  Fragments arranged by size: 

                 680     349     312      30      30      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      93     703     874    1207    1359    1449 
   Size:         93     610     171     333     152      90

  Fragments arranged by size: 

                 610     333     171     152      93      90
 
BseYI C'CCAG_C

Cuts at:      0     877    1449 
   Size:        877     572
 
BsiHKAI G_wGCw'C

Cuts at:      0    1273    1449 
   Size:       1273     176
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     223    1273    1321    1345    1449 
   Size:         86     137    1050      48      24     104

  Fragments arranged by size: 

                1050     137     104      86      48      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     905    1249    1449 
   Size:        905     344     200

  Fragments arranged by size: 

                 905     344     200
 
BsrBI CCG'CTC

Cuts at:      0      68     755    1449 
   Size:         68     687     694

  Fragments arranged by size: 

                 694     687      68
 
BsrDI GCAATG_nn'

Cuts at:      0     776    1001    1449 
   Size:        776     225     448

  Fragments arranged by size: 

                 776     448     225
 
BsrFI r'CCGG_y

Cuts at:      0     944    1199    1449 
   Size:        944     255     250

  Fragments arranged by size: 

                 944     255     250
 
BssSI C'ACGA_G

Cuts at:      0      75    1449 
   Size:         75    1374
 
BstEII G'GTnAC_C

Cuts at:      0      12    1449 
   Size:         12    1437
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177    1380    1449 
   Size:        177    1203      69

  Fragments arranged by size: 

                1203     177      69
 
BstYI r'GATC_y

Cuts at:      0     692     713     917    1449 
   Size:        692      21     204     532

  Fragments arranged by size: 

                 692     532     204      21
 
Bsu36I CC'TnA_GG

Cuts at:      0     439     670    1449 
   Size:        439     231     779

  Fragments arranged by size: 

                 779     439     231
 
BtgI C'CryG_G

Cuts at:      0     740    1379    1449 
   Size:        740     639      70

  Fragments arranged by size: 

                 740     639      70
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      21    1449 
   Size:         21    1428
 
EaeI y'GGCC_r

Cuts at:      0    1031    1449 
   Size:       1031     418
 
EarI CTCTTCn'nnn_

Cuts at:      0     680    1449 
   Size:        680     769
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     502    1449 
   Size:        502     947
 
EcoICRI GAG'CTC

Cuts at:      0    1271    1449 
   Size:       1271     178
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     253     355     376     472     496     502     649 
   Size:         42     211     102      21      96      24       6     147

Cuts at:    649     934    1012    1087    1204    1225    1305    1329    1338 
   Size:        285      78      75     117      21      80      24       9

Cuts at:   1338    1356    1449 
   Size:         18      93

  Fragments arranged by size: 

                 285     211     147     117     102      96      93      80
                  78      75      42      24      24      21      21      18
                   9       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     966    1284    1449 
   Size:        966     318     165

  Fragments arranged by size: 

                 966     318     165
 
FspI TGC'GCA

Cuts at:      0     262    1449 
   Size:        262    1187
 
FspAI rTGC'GCAy

Cuts at:      0     262    1449 
   Size:        262    1187
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     616     648     691     723    1449 
   Size:        616      32      43      32     726

  Fragments arranged by size: 

                 726     616      43      32      32
 
HincII GTy'rAC

Cuts at:      0     204    1449 
   Size:        204    1245
 
MscI TGG'CCA

Cuts at:      0    1033    1449 
   Size:       1033     416
 
MslI CAynn'nnrTG

Cuts at:      0      10     727     890    1449 
   Size:         10     717     163     559

  Fragments arranged by size: 

                 717     559     163      10
 
MspA1I CmG'CkG

Cuts at:      0     173     450    1083    1110    1250    1449 
   Size:        173     277     633      27     140     199

  Fragments arranged by size: 

                 633     277     199     173     140      27
 
NaeI GCC'GGC

Cuts at:      0     946    1201    1449 
   Size:        946     255     248

  Fragments arranged by size: 

                 946     255     248
 
NcoI C'CATG_G

Cuts at:      0     740    1449 
   Size:        740     709
 
NgoMIV G'CCGG_C

Cuts at:      0     944    1199    1449 
   Size:        944     255     250

  Fragments arranged by size: 

                 944     255     250
 
NspI r_CATG'y

Cuts at:      0     417     504     843    1449 
   Size:        417      87     339     606

  Fragments arranged by size: 

                 606     417     339      87
 
PciI A'CATG_T

Cuts at:      0     413    1449 
   Size:        413    1036
 
PfoI T'CCnGG_A

Cuts at:      0     230     332     695    1449 
   Size:        230     102     363     754

  Fragments arranged by size: 

                 754     363     230     102
 
PmlI CAC'GTG

Cuts at:      0    1405    1449 
   Size:       1405      44
 
PspOMI G'GGCC_C

Cuts at:      0      82    1449 
   Size:         82    1367
 
PspXI vC'TCGA_Gb

Cuts at:      0     105    1449 
   Size:        105    1344
 
PstI C_TGCA'G

Cuts at:      0     380     707     869    1082    1449 
   Size:        380     327     162     213     367

  Fragments arranged by size: 

                 380     367     327     213     162
 
PvuII CAG'CTG

Cuts at:      0     450    1110    1449 
   Size:        450     660     339

  Fragments arranged by size: 

                 660     450     339
 
SacI G_AGCT'C

Cuts at:      0    1273    1449 
   Size:       1273     176
 
SfcI C'TryA_G

Cuts at:      0     376     703     865    1078    1170    1308    1449 
   Size:        376     327     162     213      92     138     141

  Fragments arranged by size: 

                 376     327     213     162     141     138      92
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     974    1449 
   Size:        974     475
 
SmaI CCC'GGG

Cuts at:      0      52    1449 
   Size:         52    1397
 
SmlI C'TyrA_G

Cuts at:      0     105    1449 
   Size:        105    1344
 
StyI C'CwwG_G

Cuts at:      0     740    1449 
   Size:        740     709
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     280     667    1449 
   Size:        280     387     782

  Fragments arranged by size: 

                 782     387     280
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     349     381    1449 
   Size:        349      32    1068

  Fragments arranged by size: 

                1068     349      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1095    1449 
   Size:       1095     354
 
XhoI C'TCGA_G

Cuts at:      0     105    1449 
   Size:        105    1344
 
XmaI C'CCGG_G

Cuts at:      0      50    1449 
   Size:         50    1399
 
ZraI GAC'GTC

Cuts at:      0    1437    1449 
   Size:       1437      12
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AleI     AloI    AlwNI     ApaI
     ApoI     AvaI    BanII     BbsI    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    FspAI
    Hin4I   HincII     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     NspI     PciI     PfoI     PmlI   PspOMI    PspXI     PstI    PvuII
     SacI     SfcI     SfiI     SmaI     SmlI     StyI    TaqII     TstI
     XcmI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BciVI     BclI    BfrBI    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaBI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XmnI
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