Restriction map for pF1KB9948
 (Linear) (Six-base) MAPSORT of: KIBB9948.seq  Check: 2390  from: 1  to: 2019



KIBB9948.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     409    2019 
   Size:        409    1610
 
AatII G_ACGT'C

Cuts at:      0     515    2019 
   Size:        515    1504
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781    1138    1784    2019 
   Size:        781     357     646     235

  Fragments arranged by size: 

                 781     646     357     235
 
AfeI AGC'GCT

Cuts at:      0     424    2019 
   Size:        424    1595
 
AflIII A'CryG_T

Cuts at:      0     320    2019 
   Size:        320    1699
 
AleI CACnn'nnGTG

Cuts at:      0    1162    2019 
   Size:       1162     857
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1084    2019 
   Size:       1084     935
 
AlwNI CAG_nnn'CTG

Cuts at:      0     155    2019 
   Size:        155    1864
 
ApaLI G'TGCA_C

Cuts at:      0     220     415    1155    2019 
   Size:        220     195     740     864

  Fragments arranged by size: 

                 864     740     220     195
 
ApoI r'AATT_y

Cuts at:      0     112     238     910     979    1963    1993    2019 
   Size:        112     126     672      69     984      30      26

  Fragments arranged by size: 

                 984     672     126     112      69      30      26
 
AvaI C'yCGr_G

Cuts at:      0    1528    2019 
   Size:       1528     491
 
BanI G'GyrC_C

Cuts at:      0      70     230    1614    2019 
   Size:         70     160    1384     405

  Fragments arranged by size: 

                1384     405     160      70
 
BanII G_rGCy'C

Cuts at:      0    1100    1322    2019 
   Size:       1100     222     697

  Fragments arranged by size: 

                1100     697     222
 
BbeI G_GCGC'C

Cuts at:      0      74    2019 
   Size:         74    1945
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     637     720    2019 
   Size:        637      83    1299

  Fragments arranged by size: 

                1299     637      83
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1519    1553    2019 
   Size:       1519      34     466

  Fragments arranged by size: 

                1519     466      34
 
BfrBI ATG'CAT

Cuts at:      0    1792    2019 
   Size:       1792     227
 
BglI GCCn_nnn'nGGC

Cuts at:      0     452    2019 
   Size:        452    1567
 
Bme1580I G_kGCm'C

Cuts at:      0     224     419    1159    2019 
   Size:        224     195     740     860

  Fragments arranged by size: 

                 860     740     224     195
 
BmgBI CAC'GTC

Cuts at:      0    1149    2019 
   Size:       1149     870
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312    1496    2019 
   Size:        312    1184     523

  Fragments arranged by size: 

                1184     523     312
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     995    2019 
   Size:        995    1024
 
BsaAI yAC'GTr

Cuts at:      0     323     585    1266    2019 
   Size:        323     262     681     753

  Fragments arranged by size: 

                 753     681     323     262
 
BsaHI Gr'CG_yC

Cuts at:      0      71     512    2019 
   Size:         71     441    1507

  Fragments arranged by size: 

                1507     441      71
 
BsaWI w'CCGG_w

Cuts at:      0    1004    1799    2019 
   Size:       1004     795     220

  Fragments arranged by size: 

                1004     795     220
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     958     988    2019 
   Size:        310      30     618      30    1031

  Fragments arranged by size: 

                1031     618     310      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      55    2019 
   Size:         55    1964
 
BseYI C'CCAG_C

Cuts at:      0     442    1041    1211    1606    2019 
   Size:        442     599     170     395     413

  Fragments arranged by size: 

                 599     442     413     395     170
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141    2019 
   Size:        141    1878
 
BsiEI CG_ry'CG

Cuts at:      0     480    1308    1890    2019 
   Size:        480     828     582     129

  Fragments arranged by size: 

                 828     582     480     129
 
BsiHKAI G_wGCw'C

Cuts at:      0      49     224     419    1100    1159    2019 
   Size:         49     175     195     681      59     860

  Fragments arranged by size: 

                 860     681     195     175      59      49
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     224     419    1100    1159    1322    2019 
   Size:         49     175     195     681      59     163     697

  Fragments arranged by size: 

                 697     681     195     175     163      59      49
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     409    2019 
   Size:        409    1610
 
BsrFI r'CCGG_y

Cuts at:      0    1307    2019 
   Size:       1307     712
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     155    1348    2019 
   Size:        155    1193     671

  Fragments arranged by size: 

                1193     671     155
 
BstBI TT'CG_AA

Cuts at:      0    1967    2019 
   Size:       1967      52
 
BstYI r'GATC_y

Cuts at:      0     314     576     769    1112    1835    2019 
   Size:        314     262     193     343     723     184

  Fragments arranged by size: 

                 723     343     314     262     193     184
 
Bsu36I CC'TnA_GG

Cuts at:      0      76    2019 
   Size:         76    1943
 
BtgI C'CryG_G

Cuts at:      0     474    1235    1562    2019 
   Size:        474     761     327     457

  Fragments arranged by size: 

                 761     474     457     327
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1506    2019 
   Size:       1506     513
 
BtsI GCAGTG_nn'

Cuts at:      0     153     358    2019 
   Size:        153     205    1661

  Fragments arranged by size: 

                1661     205     153
 
DraI TTT'AAA

Cuts at:      0    2000    2019 
   Size:       2000      19
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     511    1936    2019 
   Size:        511    1425      83

  Fragments arranged by size: 

                1425     511      83
 
EaeI y'GGCC_r

Cuts at:      0     477    1305    1706    2019 
   Size:        477     828     401     313

  Fragments arranged by size: 

                 828     477     401     313
 
EagI C'GGCC_G

Cuts at:      0     477    1305    2019 
   Size:        477     828     714

  Fragments arranged by size: 

                 828     714     477
 
EarI CTCTTCn'nnn_

Cuts at:      0    1651    2019 
   Size:       1651     368
 
EcoICRI GAG'CTC

Cuts at:      0    1098    2019 
   Size:       1098     921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     781    1138    1496    1784    2019 
   Size:        312     469     357     358     288     235

  Fragments arranged by size: 

                 469     358     357     312     288     235
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1120    2019 
   Size:       1120     899
 
EcoO109I rG'GnC_Cy

Cuts at:      0     945    2019 
   Size:        945    1074
 
EcoRI G'AATT_C

Cuts at:      0    1963    2019 
   Size:       1963      56
 
EcoRV GAT'ATC

Cuts at:      0    1779    2019 
   Size:       1779     240
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1218    2019 
   Size:       1218     801
 
HaeII r_GCGC'y

Cuts at:      0      74     266     426     474    2019 
   Size:         74     192     160      48    1545

  Fragments arranged by size: 

                1545     192     160      74      48
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     591     623    2019 
   Size:        591      32    1396

  Fragments arranged by size: 

                1396     591      32
 
HincII GTy'rAC

Cuts at:      0    1986    2019 
   Size:       1986      33
 
HindIII A'AGCT_T

Cuts at:      0    1859    2019 
   Size:       1859     160
 
HpaI GTT'AAC

Cuts at:      0    1986    2019 
   Size:       1986      33
 
KasI G'GCGC_C

Cuts at:      0      70    2019 
   Size:         70    1949
 
MscI TGG'CCA

Cuts at:      0    1708    2019 
   Size:       1708     311
 
MslI CAynn'nnrTG

Cuts at:      0     403    1162    2019 
   Size:        403     759     857

  Fragments arranged by size: 

                 857     759     403
 
MspA1I CmG'CkG

Cuts at:      0     476     690    2019 
   Size:        476     214    1329

  Fragments arranged by size: 

                1329     476     214
 
NarI GG'CG_CC

Cuts at:      0      71    2019 
   Size:         71    1948
 
NcoI C'CATG_G

Cuts at:      0    1235    2019 
   Size:       1235     784
 
NotI GC'GGCC_GC

Cuts at:      0     477    2019 
   Size:        477    1542
 
NsiI A_TGCA'T

Cuts at:      0    1794    2019 
   Size:       1794     225
 
NspI r_CATG'y

Cuts at:      0    1461    1792    2019 
   Size:       1461     331     227

  Fragments arranged by size: 

                1461     331     227
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1236    2019 
   Size:       1236     783
 
PfoI T'CCnGG_A

Cuts at:      0     947    2019 
   Size:        947    1072
 
PpuMI rG'GwC_Cy

Cuts at:      0     945    2019 
   Size:        945    1074
 
PshAI GACnn'nnGTC

Cuts at:      0     272    2019 
   Size:        272    1747
 
PsiI TTA'TAA

Cuts at:      0    1850    2019 
   Size:       1850     169
 
PstI C_TGCA'G

Cuts at:      0     151    1344    2019 
   Size:        151    1193     675

  Fragments arranged by size: 

                1193     675     151
 
PvuII CAG'CTG

Cuts at:      0     690    2019 
   Size:        690    1329
 
SacI G_AGCT'C

Cuts at:      0    1100    2019 
   Size:       1100     919
 
SacII CC_GC'GG

Cuts at:      0     477    2019 
   Size:        477    1542
 
ScaI AGT'ACT

Cuts at:      0     853    2019 
   Size:        853    1166
 
SfcI C'TryA_G

Cuts at:      0     147    1340    2019 
   Size:        147    1193     679

  Fragments arranged by size: 

                1193     679     147
 
SfoI GGC'GCC

Cuts at:      0      72    2019 
   Size:         72    1947
 
SnaBI TAC'GTA

Cuts at:      0     585    2019 
   Size:        585    1434
 
SphI G_CATG'C

Cuts at:      0    1792    2019 
   Size:       1792     227
 
SspI AAT'ATT

Cuts at:      0    1950    2019 
   Size:       1950      69
 
StyI C'CwwG_G

Cuts at:      0     941    1235    1694    2019 
   Size:        941     294     459     325

  Fragments arranged by size: 

                 941     459     325     294
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1033    1345    1751    1904    2019 
   Size:       1033     312     406     153     115

  Fragments arranged by size: 

                1033     406     312     153     115
 
TatI w'GTAC_w

Cuts at:      0     851    1247    2019 
   Size:        851     396     772

  Fragments arranged by size: 

                 851     772     396
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     310     342     378     410    1890    1922    2019 
   Size:        310      32      36      32    1480      32      97

  Fragments arranged by size: 

                1480     310      97      36      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1701    2019 
   Size:       1701     318
 
XmnI GAAnn'nnTTC

Cuts at:      0     760    2019 
   Size:        760    1259
 
ZraI GAC'GTC

Cuts at:      0     513    2019 
   Size:        513    1506
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BcgI
    BfrBI     BglI Bme1580I    BmgBI     BpmI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrFI   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MscI     MslI   MspA1I     NarI     NcoI
     NotI     NsiI     NspI    PflMI     PfoI    PpuMI    PshAI     PsiI
     PstI    PvuII     SacI    SacII     ScaI     SfcI     SfoI    SnaBI
     SphI     SspI     StyI    TaqII     TatI     TstI     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI    BciVI
     BclI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaBI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstEII    BstXI  BstZ17I     ClaI    CspCI
   DraIII     EciI     FseI     FspI    FspAI     KpnI     MfeI     MluI
     MmeI     NaeI     NdeI   NgoMIV     NheI     NruI     PacI     PasI
     PciI     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SrfI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI
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