Restriction map for pF1KB4347
 (Circular) (Six-base) MAPSORT of: pF1KB4347.seq  Check: 2966  from: 1  to: 3749



pF1KB4347  3749 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AccI GT'mk_AC

Cuts at:    787     787 
   Size:       3749
 
Acc65I G'GTAC_C

Cuts at:    765     765 
   Size:       3749
 
AclI AA'CG_TT

Cuts at:   3589    3589 
   Size:       3749
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    262    1574    2006    2753    3021     262 
   Size:       1312     432     747     268     990

  Fragments arranged by size: 

                1312     990     747     432     268
 
AflIII A'CryG_T

Cuts at:   1101    2205    3182    1101 
   Size:       1104     977    1668

  Fragments arranged by size: 

                1668    1104     977
 
AgeI A'CCGG_T

Cuts at:   2137    2137 
   Size:       3749
 
AlwNI CAG_nnn'CTG

Cuts at:   1054    2621    1054 
   Size:       1567    2182
 
ApaLI G'TGCA_C

Cuts at:   2519    2519 
   Size:       3749
 
ApoI r'AATT_y

Cuts at:    605     617     753    3102    3358     605 
   Size:         12     136    2349     256     996

  Fragments arranged by size: 

                2349     996     256     136      12
 
AseI AT'TA_AT

Cuts at:     20     590     641      20 
   Size:        570      51    3128

  Fragments arranged by size: 

                3128     570      51
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
AvaI C'yCGr_G

Cuts at:    769     769 
   Size:       3749
 
BamHI G'GATC_C

Cuts at:    774     774 
   Size:       3749
 
BanI G'GyrC_C

Cuts at:    765    1179    1427    1462     765 
   Size:        414     248      35    3052

  Fragments arranged by size: 

                3052     414     248      35
 
BanII G_rGCy'C

Cuts at:    763    1793     763 
   Size:       1030    2719
 
BbeI G_GCGC'C

Cuts at:   1183    1431    1183 
   Size:        248    3501
 
BbvCI CC'TCA_GC

Cuts at:    674     674 
   Size:       3749
 
BciVI GTATCCnnnnn_n'

Cuts at:    282    1647    2408    3749     282 
   Size:       1365     761    1341     282

  Fragments arranged by size: 

                1365    1341     761     282
 
BclI T'GATC_A

Cuts at:   1269    1269 
   Size:       3749
 
BglII A'GATC_T

Cuts at:   1264    3301    1264 
   Size:       2037    1712
 
BlpI GC'TnA_GC

Cuts at:    861     861 
   Size:       3749
 
Bme1580I G_kGCm'C

Cuts at:   1374    1467    2523    1374 
   Size:         93    1056    2600

  Fragments arranged by size: 

                2600    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    961    1042    1377    3521     961 
   Size:         81     335    2144    1189

  Fragments arranged by size: 

                2144    1189     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2116    2116 
   Size:       3749
 
Bpu10I CC'TnA_GC

Cuts at:    674     674 
   Size:       3749
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    925    1804    2296    2594     925 
   Size:        879     492     298    2080

  Fragments arranged by size: 

                2080     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3453    3453 
   Size:       3749
 
BsaAI yAC'GTr

Cuts at:   1102    1102 
   Size:       3749
 
BsaBI GATnn'nnATC

Cuts at:      6    1263    3300       6 
   Size:       1257    2037     455

  Fragments arranged by size: 

                2037    1257     455
 
BsaHI Gr'CG_yC

Cuts at:   1180    1428    2130    3626    1180 
   Size:        248     702    1496    1303

  Fragments arranged by size: 

                1496    1303     702     248
 
BsaWI w'CCGG_w

Cuts at:    938    1459    2137    2411    2558    2969     938 
   Size:        521     678     274     147     411    1718

  Fragments arranged by size: 

                1718     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    727    1174    1932    2509    3005     727 
   Size:        447     758     577     496    1471

  Fragments arranged by size: 

                1471     758     577     496     447
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    362     362 
   Size:       3749
 
BsiEI CG_ry'CG

Cuts at:    104    1291    1337    2545     104 
   Size:       1187      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1187      46
 
BsiHKAI G_wGCw'C

Cuts at:    243     763    1541    1731    2523     243 
   Size:        520     778     190     792    1469

  Fragments arranged by size: 

                1469     792     778     520     190
 
BsiWI C'GTAC_G

Cuts at:    113     113 
   Size:       3749
 
BsmI GAATG_Cn'

Cuts at:    426     426 
   Size:       3749
 
Bsp1286I G_dGCh'C

Cuts at:    243     763    1374    1467    1541    1731    1793    2523     243 
   Size:        520     611      93      74     190      62     730    1469

  Fragments arranged by size: 

                1469     730     611     520     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2969    2969 
   Size:       3749
 
BspHI T'CATG_A

Cuts at:   2925    2925 
   Size:       3749
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    799    1013    1315    1696     799 
   Size:        214     302     381    2852

  Fragments arranged by size: 

                2852     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2041    2095    3579    3744      50 
   Size:       1991      54    1484     165      55

  Fragments arranged by size: 

                1991    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1661    1661 
   Size:       3749
 
BsrFI r'CCGG_y

Cuts at:   1747    1928    2137    1747 
   Size:        181     209    3359

  Fragments arranged by size: 

                3359     209     181
 
BssSI C'ACGA_G

Cuts at:    521    2020    2378     521 
   Size:       1499     358    1892

  Fragments arranged by size: 

                1892    1499     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    700    1126     700 
   Size:        426    3323
 
BstBI TT'CG_AA

Cuts at:   2110    2110 
   Size:       3749
 
BstYI r'GATC_y

Cuts at:      1     774    1241    1264    1599    1845    2857    2943    2955 
   Size:        773     467      23     335     246    1012      86      12

Cuts at:   2955    3301       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     773     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3749
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1699    1880    2043    1699 
   Size:        181     163    3405

  Fragments arranged by size: 

                3405     181     163
 
BtsI GCAGTG_nn'

Cuts at:    992     992 
   Size:       3749
 
DraI TTT'AAA

Cuts at:    748     748 
   Size:       3749
 
DrdI GACnn_nn'nnGTC

Cuts at:   1455    2313    1455 
   Size:        858    2891
 
EaeI y'GGCC_r

Cuts at:   1334    1508    1899    1926    3271    1334 
   Size:        174     391      27    1345    1812

  Fragments arranged by size: 

                1812    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1334    1334 
   Size:       3749
 
EarI CTCTTCn'nnn_

Cuts at:    130     480     572    1772    1982     130 
   Size:        350      92    1200     210    1897

  Fragments arranged by size: 

                1897    1200     350     210      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1957    2267    2413    3559    1957 
   Size:        310     146    1146    2147

  Fragments arranged by size: 

                2147    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    761     761 
   Size:       3749
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    262    1574    2006    2753    3021     262 
   Size:       1312     432     747     268     990

  Fragments arranged by size: 

                1312     990     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    888     888 
   Size:       3749
 
EcoRI G'AATT_C

Cuts at:    753     753 
   Size:       3749
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1768    1768 
   Size:       3749
 
FspI TGC'GCA

Cuts at:   1530    1530 
   Size:       3749
 
HaeII r_GCGC'y

Cuts at:   1183    1431    2453    3432    1183 
   Size:        248    1022     979    1500

  Fragments arranged by size: 

                1500    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    463     495    1591    1621    1623    1653     463 
   Size:         32    1096      30       2      30    2559

  Fragments arranged by size: 

                2559    1096      32      30      30       2
 
HincII GTy'rAC

Cuts at:    788     788 
   Size:       3749
 
HindIII A'AGCT_T

Cuts at:    512     512 
   Size:       3749
 
KasI G'GCGC_C

Cuts at:   1179    1427    1179 
   Size:        248    3501
 
KpnI G_GTAC'C

Cuts at:    769     769 
   Size:       3749
 
MluI A'CGCG_T

Cuts at:   3182    3182 
   Size:       3749
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    461    2420    2604     461 
   Size:       1959     184    1606

  Fragments arranged by size: 

                1959    1606     184
 
MscI TGG'CCA

Cuts at:   1510    1510 
   Size:       3749
 
MslI CAynn'nnrTG

Cuts at:    541    1738    1865     541 
   Size:       1197     127    2425

  Fragments arranged by size: 

                2425    1197     127
 
MspA1I CmG'CkG

Cuts at:    860    1174    1534    2547    2792     860 
   Size:        314     360    1013     245    1817

  Fragments arranged by size: 

                1817    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1930    1930 
   Size:       3749
 
NarI GG'CG_CC

Cuts at:   1180    1428    1180 
   Size:        248    3501
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3749
 
NgoMIV G'CCGG_C

Cuts at:   1928    1928 
   Size:       3749
 
NspI r_CATG'y

Cuts at:    682     703     802    1302    2209     682 
   Size:         21      99     500     907    2222

  Fragments arranged by size: 

                2222     907     500      99      21
 
PciI A'CATG_T

Cuts at:   2205    2205 
   Size:       3749
 
PmeI GTTT'AAAC

Cuts at:    748     748 
   Size:       3749
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    518     550    2888    2920     518 
   Size:         32    2338      32    1347

  Fragments arranged by size: 

                2338    1347      32      32
 
PstI C_TGCA'G

Cuts at:    796    1481     796 
   Size:        685    3064
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3749
 
PvuII CAG'CTG

Cuts at:   1174    1534    1174 
   Size:        360    3389
 
RsrII CG'GwC_CG

Cuts at:   1944    1944 
   Size:       3749
 
SacI G_AGCT'C

Cuts at:    763     763 
   Size:       3749
 
SalI G'TCGA_C

Cuts at:    786     786 
   Size:       3749
 
SapI GCTCTTCn'nnn_

Cuts at:   1772    1982    1772 
   Size:        210    3539
 
SbfI CC_TGCA'GG

Cuts at:    796     796 
   Size:       3749
 
ScaI AGT'ACT

Cuts at:   3266    3266 
   Size:       3749
 
SexAI A'CCwGG_T

Cuts at:    403     403 
   Size:       3749
 
SfcI C'TryA_G

Cuts at:     33     178     792    1477    2470    2661      33 
   Size:        145     614     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     614     191     145
 
SfoI GGC'GCC

Cuts at:   1181    1429    1181 
   Size:        248    3501
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3749
 
SmaI CCC'GGG

Cuts at:    771     771 
   Size:       3749
 
SmlI C'TyrA_G

Cuts at:    904    1819    2311    2573     904 
   Size:        915     492     262    2080

  Fragments arranged by size: 

                2080     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     357      67 
   Size:        290    3459
 
SphI G_CATG'C

Cuts at:    682     802    1302     682 
   Size:        120     500    3129

  Fragments arranged by size: 

                3129     500     120
 
StyI C'CwwG_G

Cuts at:    107     883    1237     107 
   Size:        776     354    2619

  Fragments arranged by size: 

                2619     776     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1332    1465    1924    2133    1332 
   Size:        133     459     209    2948

  Fragments arranged by size: 

                2948     459     209     133
 
TatI w'GTAC_w

Cuts at:    131    3264     131 
   Size:       3133     616
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3266    3298    3266 
   Size:         32    3717
 
Tth111I GACn'n_nGTC

Cuts at:   1546    1546 
   Size:       3749
 
XbaI T'CTAG_A

Cuts at:    481     780     481 
   Size:        299    3450
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    438    1244     438 
   Size:        806    2943
 
XmaI C'CCGG_G

Cuts at:    769     769 
   Size:       3749
 
XmnI GAAnn'nnTTC

Cuts at:    308     617     308 
   Size:        309    3440
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI    BsaXI    BseRI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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