Restriction map for pF1KB4172
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BpmI        BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NdeII       
NgoMIV      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        Sau3AI      SbfI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TfiI        Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          472  GTCGAC               GT^[AC][GT]AC
AcsI          375  AAATTT               [GA]^AATT[CT]
ApoI          375  AAATTT               [GA]^AATT[CT]
AseI          796  ATTAAT               AT^TAAT
AsnI          796  ATTAAT               AT^TAAT
AspI          475  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          561  TGGCCA               TGG^CCA
BbsI          103  GAAGAC               GAAGAC(2/6)
BbvI          988  GCTGC                GCAGC(8/12)
BcnI          574  CCCGG                CC^[GC]GG
BglI          554  GCCTAAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          691  GCTCAGC              GC^T[ACGT]AGC
BmyI          808  GGGCAC               G[AGT]GC[ACT]^C
Bpu1102I      691  GCTCAGC              GC^T[ACGT]AGC
BpuAI         103  GAAGAC               GAAGAC(2/6)
BsaI         1044  GGTCTC               GGTCTC(1/5)
BsiYI         814  CCTGGAACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          814  CCTGGAACAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      808  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      839  TGTACA               T^GTACA
BspMI        1041  GCAGGT               ACCTGC(4/8)
BsrGI         839  TGTACA               T^GTACA
BstXI         477  CCAGGTCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         691  GCTCAGC              GC^T[ACGT]AGC
EaeI          561  TGGCCA               [CT]^GGCC[GA]
EcoNI         814  CCTGGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        988  GCTGC                GC^[ACGT]GC
HapII         575  CCGG                 C^CGG
HindIII       205  AAGCTT               A^AGCTT
HpaII         575  CCGG                 C^CGG
ItaI          988  GCTGC                GC^[ACGT]GC
MluNI         561  TGGCCA               TGG^CCA
MscI          561  TGGCCA               TGG^CCA
MslI          906  CACCAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          575  CCGG                 C^CGG
NciI          574  CCCGG                CC^[GC]GG
NcoI          992  CCATGG               C^CATGG
PflFI         475  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         796  ATTAAT               AT^TAAT
PstI         1039  CTGCAG               CTGCA^G
PvuII         649  CAGCTG               CAG^CTG
SalI          472  GTCGAC               G^TCGAC
ScaI         1035  AGTACT               AGT^ACT
SexAI         476  ACCAGGT              A^CC[AT]GGT
SfcI         1039  CTGCAG               C^T[GA][CT]AG
SfiI          553  GGCCTAAATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         839  TGTACA               T^GTACA
StuI          552  AGGCCT               AGG^CCT
TseI          988  GCTGC                G^C[AT]GC
Tth111I       475  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI          685  CCGC                 CCGC(-3/-1)
              389  GCGG                
AvaII          49  GGTCC                G^G[AT]CC
              682  GGACC               
BanI          577  GGTGCC               G^G[CT][GA]CC
              809  GGCACC              
BfaI          688  CTAG                 C^TAG
             1004  CTAG                
DdeI          140  CTCAG                C^T[ACGT]AG
              692  CTCAG               
DsaI          581  CCACGG               C^C[GA][CT]GG
              992  CCATGG              
EcoT14I       269  CCAAGG               C^C[AT][AT]GG
              992  CCATGG              
HgaI          446  GACGC                GACGC(5/10)
              856  GACGC               
MaeI          688  CTAG                 C^TAG
             1004  CTAG                
MboII         103  GAAGA                GAAGA(8/7)
              538  GAAGA               
MspA1I        387  CAGCGG               C[AC]G^C[GT]G
              649  CAGCTG              
NheI          687  GCTAGC               G^CTAGC
             1003  GCTAGC              
NlaIII        147  CATG                 CATG^
              993  CATG                
SfaNI         274  GATGC                GCATC(5/9)
              370  GATGC               
StyI          269  CCAAGG               C^C[AT][AT]GG
              992  CCATGG              
TaqI          473  TCGA                 T^CGA
              513  TCGA                
TspRI         483  CACTG                CA[GC]TG[ACGT][ACGT]^
              594  CACTG               
TthHB8I       473  TCGA                 T^CGA
              513  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AfaI        AluI        BciVI       BsaJI       BsmAI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      EcoRII      FokI        HaeIII      HincII      
HindII      HinfI       HphI        MaeIII      MnlI        
MseI        MvaI        MwoI        NlaIV       PleI        
RsaI        Sau96I      ScrFI       Tru9I       Tsp45I      
Tsp509I     
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