Restriction map for pF1KB3668
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HpaI        HphI        KpnI        
Ksp632I     KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfaNI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AcyI           25  GGCGCC               G[GA]^CG[CT]C
AflIII        511  ACGTGT               A^C[GA][CT]GT
Asp700I       196  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          61  GAGCAC               G[AT]GC[AT]^C
AvaI           14  CCCGGG               C^[CT]CG[GA]G
AvaII         331  GGTCC                G^G[AT]CC
BbeI           25  GGCGCC               GGCGC^C
BbsI          395  GAAGAC               GAAGAC(2/6)
BbvI           56  GCTGC                GCAGC(8/12)
BmyI           61  GAGCAC               G[AGT]GC[ACT]^C
BpmI          752  CTGGAG               CTGGAG(16/14)
BpuAI         395  GAAGAC               GAAGAC(2/6)
BsaHI          25  GGCGCC               G[GA]^CG[CT]C
BsaJI          14  CCCGGG               C^C[ACGT][ACGT]GG
BsgI          178  GTGCAG               GTGCAG(16/14)
BsiHKAI        61  GAGCAC               G[AT]GC[AT]^C
BsiYI         581  CCAGGTATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          581  CCAGGTATTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         420  GTCCC                GGGAC(10/14)
BsoBI          14  CCCGGG               C^[CT]CG[GA]G
Bsp1286I       61  GAGCAC               G[AGT]GC[ACT]^C
BspHI         129  TCATGA               T^CATGA
BsrDI         665  GCAATG               GCAATG(2/0)
BsrI          771  ACTGG                ACTGG(1/-1)
BstNI         581  CCAGG                CC^[AT]GG
CfoI           26  GCGC                 GCG^C
DraI          163  TTTAAA               TTT^AAA
DrdI          425  GACAGAATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        581  CCAGG                ^CC[AT]GG
FauI           51  GCGGG                CCCGC(4/6)
HaeII          25  GGCGCC               [GA]GCGC^[CT]
HaeIII         12  GGCC                 GG^CC
HapII          15  CCGG                 C^CGG
HhaI           26  GCGC                 GCG^C
Hin1I          25  GGCGCC               G[GA]^CG[CT]C
HinP1I         26  GCGC                 G^CGC
HindIII       106  AAGCTT               A^AGCTT
HpaII          15  CCGG                 C^CGG
KasI           25  GGCGCC               G^GCGCC
MseI          164  TTAA                 T^TAA
MslI          645  CATGACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           15  CCGG                 C^CGG
MvaI          581  CCAGG                CC^[AT]GG
NarI           25  GGCGCC               GG^CGCC
NspI          207  ACATGC               [GA]CATG^[CT]
PflMI         581  CCAGGTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          618  GACTC                GAGTC(4/5)
PvuII         595  CAGCTG               CAG^CTG
RcaI          129  TCATGA               T^CATGA
SexAI         580  ACCAGGT              A^CC[AT]GGT
SfcI          540  CTACAG               C^T[GA][CT]AG
SfoI           25  GGCGCC               GGC^GCC
SmaI           14  CCCGGG               CCC^GGG
SmlI          746  CTCAAG               C^T[CT][GA]AG
SspI          251  AATATT               AAT^ATT
Tru9I         164  TTAA                 T^TAA
TseI           56  GCTGC                G^C[AT]GC
Tsp45I        101  GTGAC                ^GT[GC]AC
Van91I        581  CCAGGTATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          14  CCCGGG               C^CCGGG
XmaI           14  CCCGGG               C^CCGGG
XmnI          196  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AlwI          683  GATCC                GGATC(4/5)
              774  GGATC               
BanI           25  GGCGCC               G^G[CT][GA]CC
               90  GGCACC              
BcnI           14  CCCGG                CC^[GC]GG
               15  CCGGG               
BfaI          154  CTAG                 C^TAG
              376  CTAG                
BsmAI         591  GAGAC                GTCTC(1/5)
               80  GTCTC               
Cac8I          28  GCCAGC               GC[ACGT]^[ACGT]GC
               59  GCGAGC              
Cfr13I         12  GGCCC                G^G[ACGT]CC
              331  GGTCC               
MaeI          154  CTAG                 C^TAG
              376  CTAG                
MaeII         511  ACGT                 A^CGT
              759  ACGT                
MaeIII        101  GTGAC                ^GT[ACGT]AC
              365  GTAAC               
NciI           14  CCCGG                CC^[GC]GG
               15  CCGGG               
NlaIV          25  GGCGCC               GG[ACGT]^[ACGT]CC
               90  GGCACC              
Sau96I         12  GGCCC                G^G[ACGT]CC
              331  GGTCC               
TfiI          384  GAATC                G^A[AT]TC
              730  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BssKI       
BstF5I      DpnII       Fnu4HI      FokI        HinfI       
ItaI        MboI        MboII       MnlI        MspA1I      
MwoI        NdeII       NlaIII      Sau3AI      ScrFI       
Tsp509I     TspRI       
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