Restriction map for pF1KB3469
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsiYI       BslI        BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoT14I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HaeIII      HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
ItaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TseI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          85  CGCG                 CG^CG
AcsI          193  GAATTT               [GA]^AATT[CT]
AocI          532  CCTGAGG              CC^T[ACGT]AGG
ApoI          193  GAATTT               [GA]^AATT[CT]
AspHI         139  GAGCAC               G[AT]GC[AT]^C
AvaI          341  CTCGGG               C^[CT]CG[GA]G
BcgI          329  GCAATGATATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           76  CTAG                 C^TAG
BmyI          139  GAGCAC               G[AGT]GC[ACT]^C
BsiHKAI       139  GAGCAC               G[AT]GC[AT]^C
BsmAI         510  GAGAC                GTCTC(1/5)
BsmFI          96  GGGAC                GGGAC(10/14)
BsoBI         341  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      139  GAGCAC               G[AGT]GC[ACT]^C
BsrDI         329  GCAATG               GCAATG(2/0)
BsrI          360  ACTGG                ACTGG(1/-1)
BstUI          85  CGCG                 CG^CG
Bsu36I        532  CCTGAGG              CC^T[ACGT]AGG
Cac8I         522  GCATGC               GC[ACGT]^[ACGT]GC
CfoI           84  GCGC                 GCG^C
DpnII         375  GATC                 ^GATC
EarI          505  GAAGAG               CTCTTC(1/4)
Eco81I        532  CCTGAGG              CC^T[ACGT]AGG
EcoRV         334  GATATC               GAT^ATC
EcoT22I       524  ATGCAT               ATGCA^T
HhaI           84  GCGC                 GCG^C
HinP1I         84  GCGC                 G^CGC
HinfI         391  GATTC                G^A[ACGT]TC
Ksp632I       505  GAAGAG               CTCTTC(1/4)
MaeI           76  CTAG                 C^TAG
MaeIII        260  GTAAC                ^GT[ACGT]AC
MboI          375  GATC                 ^GATC
MspA1I         23  CAGCTG               C[AC]G^C[GT]G
MvnI           85  CGCG                 CG^CG
NdeII         375  GATC                 ^GATC
NsiI          524  ATGCAT               ATGCA^T
NspI          522  GCATGC               [GA]CATG^[CT]
PvuII          23  CAGCTG               CAG^CTG
Sau3AI        375  GATC                 ^GATC
SexAI         446  ACCTGGT              A^CC[AT]GGT
SfaNI         538  GATGC                GCATC(5/9)
SphI          522  GCATGC               GCATG^C
TfiI          391  GATTC                G^A[AT]TC
TspRI         288  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI           18  GCGG                 CCGC(-3/-1)
              541  GCGG                
BsaJI         241  CCCAGG               C^C[ACGT][ACGT]GG
              242  CCAGGG              
BssKI         242  CCAGG                ^CC[ACGT]GG
              447  CCTGG               
BstF5I         45  GGATG                GGATG(2/0)
              537  GGATG               
BstNI         242  CCAGG                CC^[AT]GG
              447  CCTGG               
EcoRII        242  CCAGG                ^CC[AT]GG
              447  CCTGG               
FokI           45  GGATG                GGATG(9/13)
              537  GGATG               
HphI          226  GGTGA                GGTGA(8/7)
              400  GGTGA               
MseI           92  TTAA                 T^TAA
              406  TTAA                
MvaI          242  CCAGG                CC^[AT]GG
              447  CCTGG               
MwoI           75  GCTAGACAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              457  GCAAAAGAAGC         
NlaIII        518  CATG                 CATG^
              523  CATG                
NlaIV         153  GGAACC               GG[ACGT]^[ACGT]CC
              363  GGAACC              
ScrFI         242  CCAGG                CC^[ACGT]GG
              447  CCTGG               
Tru9I          92  TTAA                 T^TAA
              406  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AluI        BseRI       DdeI        MboII       MnlI        
Tsp509I     
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