Restriction map for pF1KB9562
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     HpaI        KpnI        KspI        MfeI        
MluI        MseI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SbfI        ScaI        
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1150  AAATTC               [GA]^AATT[CT]
ApoI         1150  AAATTC               [GA]^AATT[CT]
BamHI         794  GGATCC               G^GATCC
BbeI         1739  GGCGCC               GGCGC^C
BglII        2129  AGATCT               A^GATCT
BspHI        2090  TCATGA               T^CATGA
BspLU11I     2279  ACATGT               A^CATGT
BsrDI         444  CATTGC               GCAATG(2/0)
BstEII       1500  GGTGACC              G^GT[ACGT]ACC
BstPI        1500  GGTGACC              G^GT[ACGT]ACC
EcoO65I      1500  GGTGACC              G^GT[ACGT]ACC
HaeII        1739  GGCGCC               [GA]GCGC^[CT]
KasI         1739  GGCGCC               G^GCGCC
NarI         1739  GGCGCC               GG^CGCC
RcaI         2090  TCATGA               T^CATGA
SalI            6  GTCGAC               G^TCGAC
SfoI         1739  GGCGCC               GGC^GCC
SgrAI        1741  CGCCGGCG             C[GA]^CCGG[CT]G
SphI          408  GCATGC               GCATG^C
StuI         1532  AGGCCT               AGG^CCT
Tsp509I      1151  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AatII         517  GACGTC               GACGT^C
             1180  GACGTC              
AccI            6  GTCGAC               GT^[AC][GT]AC
             1651  GTCTAC              
AccII         307  CGCG                 CG^CG
             1336  CGCG                
AccIII         29  TCCGGA               T^CCGGA
              672  TCCGGA              
AflIII       1469  ACGTGT               A^C[GA][CT]GT
             2279  ACATGT              
AspHI         495  GAGCAC               G[AT]GC[AT]^C
              859  GTGCTC              
BfaI         1114  CTAG                 C^TAG
             1186  CTAG                
BsaBI         943  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2130  GATCTGCATC          
BseAI          29  TCCGGA               T^CCGGA
              672  TCCGGA              
BsiEI           8  CGACCG               CG[GA][CT]^CG
             2002  CGACCG              
BsiHKAI       495  GAGCAC               G[AT]GC[AT]^C
              859  GTGCTC              
BsmAI        1239  GTCTC                GTCTC(1/5)
             1764  GTCTC               
BspEI          29  TCCGGA               T^CCGGA
              672  TCCGGA              
BsrBI        1287  GAGCGG               CCGCTC(-3/-3)
             1294  GAGCGG              
BstUI         307  CGCG                 CG^CG
             1336  CGCG                
CfoI         1671  GCGC                 GCG^C
             1740  GCGC                
DraIII        201  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              762  CACCGTGTG           
HgaI           60  GACGC                GACGC(5/10)
             2288  GCGTC               
HhaI         1671  GCGC                 GCG^C
             1740  GCGC                
HinP1I       1671  GCGC                 G^CGC
             1740  GCGC                
HincII          6  GTCGAC               GT[CT]^[GA]AC
             1465  GTCAAC              
HindII          6  GTCGAC               GT[CT]^[GA]AC
             1465  GTCAAC              
MaeI         1114  CTAG                 C^TAG
             1186  CTAG                
MamI          943  GATGCCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2130  GATCTGCATC          
MroI           29  TCCGGA               T^CCGGA
              672  TCCGGA              
MvnI          307  CGCG                 CG^CG
             1336  CGCG                
PvuII         534  CAGCTG               CAG^CTG
             1687  CAGCTG              
SmaI          367  CCCGGG               CCC^GGG
             1226  CCCGGG              
XcmI          899  CCAGCCCCACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2038  CCAACGCCAGAGTGG     
XmaCI         367  CCCGGG               C^CCGGG
             1226  CCCGGG              
XmaI          367  CCCGGG               C^CCGGG
             1226  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       Hin1I       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SexAI       SfaNI       SfcI        SmlI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
Van91I      XhoII       
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