Restriction map for pF1KB9562
 (Linear) (Six-base) MAPSORT of: KIBB9562.seq  Check: 8158  from: 1  to: 2364



KIBB9562.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     818    1532    2094    2109    2364 
   Size:        818     714     562      15     255

  Fragments arranged by size: 

                 818     714     562     255      15
 
AatII G_ACGT'C

Cuts at:      0     521    1184    2364 
   Size:        521     663    1180

  Fragments arranged by size: 

                1180     663     521
 
AccI GT'mk_AC

Cuts at:      0       7    1652    2364 
   Size:          7    1645     712

  Fragments arranged by size: 

                1645     712       7
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    2364 
   Size:       1189    1175
 
AflIII A'CryG_T

Cuts at:      0    1469    2279    2364 
   Size:       1469     810      85

  Fragments arranged by size: 

                1469     810      85
 
AleI CACnn'nnGTG

Cuts at:      0     124     307     884    1262    1459    2194    2335    2364 
   Size:        124     183     577     378     197     735     141      29

  Fragments arranged by size: 

                 735     577     378     197     183     141     124      29
            
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     313    2364 
   Size:        313    2051
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     970    1000    1002    1032    2364 
   Size:        970      30       2      30    1332

  Fragments arranged by size: 

                1332     970      30      30       2
 
AlwNI CAG_nnn'CTG

Cuts at:      0     961    1110    2109    2364 
   Size:        961     149     999     255

  Fragments arranged by size: 

                 999     961     255     149
 
ApoI r'AATT_y

Cuts at:      0    1150    2364 
   Size:       1150    1214
 
AvaI C'yCGr_G

Cuts at:      0     367    1226    1241    2250    2364 
   Size:        367     859      15    1009     114

  Fragments arranged by size: 

                1009     859     367     114      15
 
BamHI G'GATC_C

Cuts at:      0     794    2364 
   Size:        794    1570
 
BanI G'GyrC_C

Cuts at:      0     147     916     928    1418    1739    1786    1831    1906 
   Size:        147     769      12     490     321      47      45      75

Cuts at:   1906    2188    2364 
   Size:        282     176

  Fragments arranged by size: 

                 769     490     321     282     176     147      75      47
                  45      12
 
BanII G_rGCy'C

Cuts at:      0    1760    1804    1934    1976    2038    2364 
   Size:       1760      44     130      42      62     326

  Fragments arranged by size: 

                1760     326     130      62      44      42
 
BbeI G_GCGC'C

Cuts at:      0    1743    2364 
   Size:       1743     621
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1260    1350    1578    1596    2364 
   Size:       1260      90     228      18     768

  Fragments arranged by size: 

                1260     768     228      90      18
 
BglII A'GATC_T

Cuts at:      0    2129    2364 
   Size:       2129     235
 
Bme1580I G_kGCm'C

Cuts at:      0     368    1343    1421    1789    2216    2257    2364 
   Size:        368     975      78     368     427      41     107

  Fragments arranged by size: 

                 975     427     368     368     107      78      41
 
BmgBI CAC'GTC

Cuts at:      0    2337    2364 
   Size:       2337      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1348    2364 
   Size:       1348    1016
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     204    1554    1806    2364 
   Size:        204    1350     252     558

  Fragments arranged by size: 

                1350     558     252     204
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     938    1045    1549    1618    1844    2364 
   Size:        360     578     107     504      69     226     520

  Fragments arranged by size: 

                 578     520     504     360     226     107      69
 
Bpu10I CC'TnA_GC

Cuts at:      0    1754    1928    1964    2364 
   Size:       1754     174      36     400

  Fragments arranged by size: 

                1754     400     174      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1009    1468    1822    2364 
   Size:       1009     459     354     542

  Fragments arranged by size: 

                1009     542     459     354
 
BsaBI GATnn'nnATC

Cuts at:      0     947    2134    2364 
   Size:        947    1187     230

  Fragments arranged by size: 

                1187     947     230
 
BsaHI Gr'CG_yC

Cuts at:      0     518    1181    1740    2288    2364 
   Size:        518     663     559     548      76

  Fragments arranged by size: 

                 663     559     548     518      76
 
BsaWI w'CCGG_w

Cuts at:      0      29     488     608     672    2364 
   Size:         29     459     120      64    1692

  Fragments arranged by size: 

                1692     459     120      64      29
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     508     538    2364 
   Size:        508      30    1826

  Fragments arranged by size: 

                1826     508      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     395     507     745     859    1018    2364 
   Size:        395     112     238     114     159    1346

  Fragments arranged by size: 

                1346     395     238     159     114     112
 
BseYI C'CCAG_C

Cuts at:      0     581     666    1934    1975    2364 
   Size:        581      85    1268      41     389

  Fragments arranged by size: 

                1268     581     389      85      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     253    1504    2364 
   Size:        148     105    1251     860

  Fragments arranged by size: 

                1251     860     148     105
 
BsiEI CG_ry'CG

Cuts at:      0      11    2005    2364 
   Size:         11    1994     359

  Fragments arranged by size: 

                1994     359      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     499     863    2364 
   Size:        499     364    1501

  Fragments arranged by size: 

                1501     499     364
 
Bsp1286I G_dGCh'C

Cuts at:      0     368     499     863    1343    1421    1760    1789    1804 
   Size:        368     131     364     480      78     339      29      15

Cuts at:   1804    1934    1976    2038    2216    2257    2364 
   Size:        130      42      62     178      41     107

  Fragments arranged by size: 

                 480     368     364     339     178     131     130     107
                  78      62      42      41      29      15
 
BspEI T'CCGG_A

Cuts at:      0      29     672    2364 
   Size:         29     643    1692

  Fragments arranged by size: 

                1692     643      29
 
BspHI T'CATG_A

Cuts at:      0    2090    2364 
   Size:       2090     274
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     461     668     818    1079    1388    1532    1706    2094 
   Size:        461     207     150     261     309     144     174     388

Cuts at:   2094    2109    2364 
   Size:         15     255

  Fragments arranged by size: 

                 461     388     309     261     255     207     174     150
                 144      15
 
BsrBI CCG'CTC

Cuts at:      0    1289    1296    2364 
   Size:       1289       7    1068

  Fragments arranged by size: 

                1289    1068       7
 
BsrDI GCAATG_nn'

Cuts at:      0     443    2364 
   Size:        443    1921
 
BsrFI r'CCGG_y

Cuts at:      0     150     293    1742    1771    2364 
   Size:        150     143    1449      29     593

  Fragments arranged by size: 

                1449     593     150     143      29
 
BstEII G'GTnAC_C

Cuts at:      0    1500    2364 
   Size:       1500     864
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     420    1633    1851    2364 
   Size:        420    1213     218     513

  Fragments arranged by size: 

                1213     513     420     218
 
BstYI r'GATC_y

Cuts at:      0     161     794    1091    2129    2165    2364 
   Size:        161     633     297    1038      36     199

  Fragments arranged by size: 

                1038     633     297     199     161      36
 
BtgI C'CryG_G

Cuts at:      0      68     694     716     935    1343    1496    1703    1793 
   Size:         68     626      22     219     408     153     207      90

Cuts at:   1793    2364 
   Size:        571

  Fragments arranged by size: 

                 626     571     408     219     207     153      90      68
                  22
 
BtsI GCAGTG_nn'

Cuts at:      0     900    2035    2255    2364 
   Size:        900    1135     220     109

  Fragments arranged by size: 

                1135     900     220     109
 
DraIII CAC_nnn'GTG

Cuts at:      0     206     767    2364 
   Size:        206     561    1597

  Fragments arranged by size: 

                1597     561     206
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1195    1354    1884    2157    2317    2364 
   Size:       1195     159     530     273     160      47

  Fragments arranged by size: 

                1195     530     273     160     159      47
 
EaeI y'GGCC_r

Cuts at:      0     197     422     440    1691    2364 
   Size:        197     225      18    1251     673

  Fragments arranged by size: 

                1251     673     225     197      18
 
EarI CTCTTCn'nnn_

Cuts at:      0     173     389     463     748     820    1126    1162    1165 
   Size:        173     216      74     285      72     306      36       3

Cuts at:   1165    1213    1444    1561    2364 
   Size:         48     231     117     803

  Fragments arranged by size: 

                 803     306     285     231     216     173     117      74
                  72      48      36       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1761    2364 
   Size:       1761     603
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     938    1045    1189    1549    1618    1844    2364 
   Size:        360     578     107     144     360      69     226     520

  Fragments arranged by size: 

                 578     520     360     360     226     144     107      69
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     841    1528    1597    2364 
   Size:        841     687      69     767

  Fragments arranged by size: 

                 841     767     687      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     330     416     958    1367    1728    2052    2145    2364 
   Size:        330      86     542     409     361     324      93     219

  Fragments arranged by size: 

                 542     409     361     330     324     219      93      86
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42    2364 
   Size:         42    2322
 
HaeII r_GCGC'y

Cuts at:      0    1743    2364 
   Size:       1743     621
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     508     540    1624    1656    1747    1779    2364 
   Size:        508      32    1084      32      91      32     585

  Fragments arranged by size: 

                1084     585     508      91      32      32      32
 
HincII GTy'rAC

Cuts at:      0       8    1467    2364 
   Size:          8    1459     897

  Fragments arranged by size: 

                1459     897       8
 
KasI G'GCGC_C

Cuts at:      0    1739    2364 
   Size:       1739     625
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1073    1667    2364 
   Size:        215     858     594     697

  Fragments arranged by size: 

                 858     697     594     215
 
MscI TGG'CCA

Cuts at:      0     199     442    1693    2364 
   Size:        199     243    1251     671

  Fragments arranged by size: 

                1251     671     243     199
 
MslI CAynn'nnrTG

Cuts at:      0     124     307     884     934    1262    1390    1459    1792 
   Size:        124     183     577      50     328     128      69     333

Cuts at:   1792    2194    2260    2278    2335    2364 
   Size:        402      66      18      57      29

  Fragments arranged by size: 

                 577     402     333     328     183     128     124      69
                  66      57      50      29      18
 
MspA1I CmG'CkG

Cuts at:      0     536    1415    1425    1689    1737    1813    1998    2079 
   Size:        536     879      10     264      48      76     185      81

Cuts at:   2079    2358    2364 
   Size:        279       6

  Fragments arranged by size: 

                 879     536     279     264     185      81      76      48
                  10       6
 
NaeI GCC'GGC

Cuts at:      0     152     295    1744    2364 
   Size:        152     143    1449     620

  Fragments arranged by size: 

                1449     620     152     143
 
NarI GG'CG_CC

Cuts at:      0    1740    2364 
   Size:       1740     624
 
NcoI C'CATG_G

Cuts at:      0     694     935    1343    1496    1793    2364 
   Size:        694     241     408     153     297     571

  Fragments arranged by size: 

                 694     571     408     297     241     153
 
NgoMIV G'CCGG_C

Cuts at:      0     150     293    1742    2364 
   Size:        150     143    1449     622

  Fragments arranged by size: 

                1449     622     150     143
 
NspI r_CATG'y

Cuts at:      0     412     641     733    2283    2364 
   Size:        412     229      92    1550      81

  Fragments arranged by size: 

                1550     412     229      92      81
 
PciI A'CATG_T

Cuts at:      0    2279    2364 
   Size:       2279      85
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     528     695    1547    1581    1689    2364 
   Size:        528     167     852      34     108     675

  Fragments arranged by size: 

                 852     675     528     167     108      34
 
PfoI T'CCnGG_A

Cuts at:      0    1595    2059    2364 
   Size:       1595     464     305

  Fragments arranged by size: 

                1595     464     305
 
PpuMI rG'GwC_Cy

Cuts at:      0     330    1367    2052    2364 
   Size:        330    1037     685     312

  Fragments arranged by size: 

                1037     685     330     312
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1309    1341    2364 
   Size:       1309      32    1023

  Fragments arranged by size: 

                1309    1023      32
 
PstI C_TGCA'G

Cuts at:      0     292    1736    1824    2084    2105    2183    2364 
   Size:        292    1444      88     260      21      78     181

  Fragments arranged by size: 

                1444     292     260     181      88      78      21
 
PvuII CAG'CTG

Cuts at:      0     536    1689    2364 
   Size:        536    1153     675

  Fragments arranged by size: 

                1153     675     536
 
SalI G'TCGA_C

Cuts at:      0       6    2364 
   Size:          6    2358
 
SanDI GG'GwC_CC

Cuts at:      0    1367    2364 
   Size:       1367     997
 
SapI GCTCTTCn'nnn_

Cuts at:      0     463     820    1165    2364 
   Size:        463     357     345    1199

  Fragments arranged by size: 

                1199     463     357     345
 
SexAI A'CCwGG_T

Cuts at:      0    1546    1580    1646    2364 
   Size:       1546      34      66     718

  Fragments arranged by size: 

                1546     718      66      34
 
SfcI C'TryA_G

Cuts at:      0     288    1732    1820    2080    2101    2179    2364 
   Size:        288    1444      88     260      21      78     185

  Fragments arranged by size: 

                1444     288     260     185      88      78      21
 
SfoI GGC'GCC

Cuts at:      0    1741    2364 
   Size:       1741     623
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1742    2364 
   Size:       1742     622
 
SmaI CCC'GGG

Cuts at:      0     369    1228    2364 
   Size:        369     859    1136

  Fragments arranged by size: 

                1136     859     369
 
SmlI C'TyrA_G

Cuts at:      0    1024    1483    1837    2364 
   Size:       1024     459     354     527

  Fragments arranged by size: 

                1024     527     459     354
 
SphI G_CATG'C

Cuts at:      0     412    2364 
   Size:        412    1952
 
SrfI GCCC'GGGC

Cuts at:      0    1228    2364 
   Size:       1228    1136
 
StuI AGG'CCT

Cuts at:      0    1534    2364 
   Size:       1534     830
 
StyI C'CwwG_G

Cuts at:      0     694     935    1343    1496    1793    2364 
   Size:        694     241     408     153     297     571

  Fragments arranged by size: 

                 694     571     408     297     241     153
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      25    1894    2364 
   Size:         25    1869     470

  Fragments arranged by size: 

                1869     470      25
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     906    2045    2364 
   Size:        906    1139     319

  Fragments arranged by size: 

                1139     906     319
 
XmaI C'CCGG_G

Cuts at:      0     367    1226    2364 
   Size:        367     859    1138

  Fragments arranged by size: 

                1138     859     367
 
ZraI GAC'GTC

Cuts at:      0     519    1182    2364 
   Size:        519     663    1182

  Fragments arranged by size: 

                1182     663     519
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BstEII    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI    PpuMI     PsrI
     PstI    PvuII     SalI    SanDI     SapI    SexAI     SfcI     SfoI
    SgrAI     SmaI     SmlI     SphI     SrfI     StuI     StyI    TaqII
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI     BmtI     BsaI    BsaAI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PvuI
    RsrII     SacI    SacII     SbfI     ScaI     SfiI     SgfI    SnaBI
     SpeI     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI
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