Restriction map for
pF1KB6883
(Circular) (Six-base) MAPSORT of: pF1KB6883.seq Check: 1597 from: 1 to: 3656
pF1KB6883 3656 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 694 694
Size: 3656
Acc65I G'GTAC_C
Cuts at: 672 672
Size: 3656
AclI AA'CG_TT
Cuts at: 3496 3496
Size: 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1481 1913 2660 2928 1481
Size: 432 747 268 2209
Fragments arranged by size:
2209 747 432 268
AflIII A'CryG_T
Cuts at: 1008 2112 3089 1008
Size: 1104 977 1575
Fragments arranged by size:
1575 1104 977
AgeI A'CCGG_T
Cuts at: 2044 2044
Size: 3656
AlwNI CAG_nnn'CTG
Cuts at: 961 2528 961
Size: 1567 2089
ApaLI G'TGCA_C
Cuts at: 2426 2426
Size: 3656
ApoI r'AATT_y
Cuts at: 277 292 638 660 3009 3265 277
Size: 15 346 22 2349 256 668
Fragments arranged by size:
2349 668 346 256 22 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 3656
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
AvaI C'yCGr_G
Cuts at: 676 676
Size: 3656
BamHI G'GATC_C
Cuts at: 681 681
Size: 3656
BanI G'GyrC_C
Cuts at: 672 1086 1334 1369 672
Size: 414 248 35 2959
Fragments arranged by size:
2959 414 248 35
BanII G_rGCy'C
Cuts at: 670 1700 670
Size: 1030 2626
BbeI G_GCGC'C
Cuts at: 1090 1338 1090
Size: 248 3408
BbsI GAAGACnn'nnnn_
Cuts at: 254 254
Size: 3656
BciVI GTATCCnnnnn_n'
Cuts at: 1554 2315 3656 1554
Size: 761 1341 1554
Fragments arranged by size:
1554 1341 761
BclI T'GATC_A
Cuts at: 1176 1176
Size: 3656
BglI GCCn_nnn'nGGC
Cuts at: 220 220
Size: 3656
BglII A'GATC_T
Cuts at: 383 506 1171 3208 383
Size: 123 665 2037 831
Fragments arranged by size:
2037 831 665 123
BlpI GC'TnA_GC
Cuts at: 133 768 133
Size: 635 3021
Bme1580I G_kGCm'C
Cuts at: 1281 1374 2430 1281
Size: 93 1056 2507
Fragments arranged by size:
2507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 868 949 1284 3428 868
Size: 81 335 2144 1096
Fragments arranged by size:
2144 1096 335 81
BmtI G_CTAG'C
Cuts at: 325 325
Size: 3656
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2023 2023
Size: 3656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 481 253
Size: 228 3428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 832 1711 2203 2501 187
Size: 645 879 492 298 1342
Fragments arranged by size:
1342 879 645 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3360 3360
Size: 3656
BsaAI yAC'GTr
Cuts at: 1009 1009
Size: 3656
BsaBI GATnn'nnATC
Cuts at: 6 1170 3207 6
Size: 1164 2037 455
Fragments arranged by size:
2037 1164 455
BsaHI Gr'CG_yC
Cuts at: 1087 1335 2037 3533 1087
Size: 248 702 1496 1210
Fragments arranged by size:
1496 1210 702 248
BsaWI w'CCGG_w
Cuts at: 845 1366 2044 2318 2465 2876 845
Size: 521 678 274 147 411 1625
Fragments arranged by size:
1625 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 424
Size: 30 3626
BseYI C'CCAG_C
Cuts at: 1081 1839 2416 2912 1081
Size: 758 577 496 1825
Fragments arranged by size:
1825 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 148 1198 1244 2452 104
Size: 44 1050 46 1208 1308
Fragments arranged by size:
1308 1208 1050 46 44
BsiHKAI G_wGCw'C
Cuts at: 211 670 1448 1638 2430 211
Size: 459 778 190 792 1437
Fragments arranged by size:
1437 792 778 459 190
Bsp1286I G_dGCh'C
Cuts at: 211 670 1281 1374 1448 1638 1700 2430 211
Size: 459 611 93 74 190 62 730 1437
Fragments arranged by size:
1437 730 611 459 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2876 2876
Size: 3656
BspHI T'CATG_A
Cuts at: 518 2832 518
Size: 2314 1342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 920 1222 1603 706
Size: 214 302 381 2759
Fragments arranged by size:
2759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1948 2002 3486 3651 50
Size: 1898 54 1484 165 55
Fragments arranged by size:
1898 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1568 1568
Size: 3656
BsrFI r'CCGG_y
Cuts at: 128 1654 1835 2044 128
Size: 1526 181 209 1740
Fragments arranged by size:
1740 1526 209 181
BssSI C'ACGA_G
Cuts at: 1927 2285 1927
Size: 358 3298
BstAPI GCAn_nnn'nTGC
Cuts at: 1033 1033
Size: 3656
BstBI TT'CG_AA
Cuts at: 2017 2017
Size: 3656
BstYI r'GATC_y
Cuts at: 1 383 458 506 681 1148 1171 1506 1752
Size: 382 75 48 175 467 23 335 246
Cuts at: 1752 2764 2850 2862 3208 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 382 346 335 246 175
86 75 48 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 185 1606 1787 1950 185
Size: 1421 181 163 1891
Fragments arranged by size:
1891 1421 181 163
BtsI GCAGTG_nn'
Cuts at: 899 899
Size: 3656
DraI TTT'AAA
Cuts at: 284 566 655 284
Size: 282 89 3285
Fragments arranged by size:
3285 282 89
DrdI GACnn_nn'nnGTC
Cuts at: 399 1362 2220 399
Size: 963 858 1835
Fragments arranged by size:
1835 963 858
EaeI y'GGCC_r
Cuts at: 145 1241 1415 1806 1833 3178 145
Size: 1096 174 391 27 1345 623
Fragments arranged by size:
1345 1096 623 391 174 27
EagI C'GGCC_G
Cuts at: 145 1241 145
Size: 1096 2560
EarI CTCTTCn'nnn_
Cuts at: 581 1679 1889 581
Size: 1098 210 2348
Fragments arranged by size:
2348 1098 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1864 2174 2320 3466 1864
Size: 310 146 1146 2054
Fragments arranged by size:
2054 1146 310 146
EcoICRI GAG'CTC
Cuts at: 668 668
Size: 3656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 481 1481 1913 2660 2928 253
Size: 228 1000 432 747 268 981
Fragments arranged by size:
1000 981 747 432 268 228
EcoO109I rG'GnC_Cy
Cuts at: 795 795
Size: 3656
EcoRI G'AATT_C
Cuts at: 292 660 292
Size: 368 3288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 573 1675 573
Size: 1102 2554
FspI TGC'GCA
Cuts at: 1437 1437
Size: 3656
HaeII r_GCGC'y
Cuts at: 1090 1338 2360 3339 1090
Size: 248 1022 979 1407
Fragments arranged by size:
1407 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 498 530 1498 1528 1530 1560 424
Size: 32 42 32 968 30 2 30 2520
Fragments arranged by size:
2520 968 42 32 32 30 30 2
HincII GTy'rAC
Cuts at: 426 695 426
Size: 269 3387
KasI G'GCGC_C
Cuts at: 1086 1334 1086
Size: 248 3408
KpnI G_GTAC'C
Cuts at: 676 676
Size: 3656
MluI A'CGCG_T
Cuts at: 3089 3089
Size: 3656
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2327 2511 2327
Size: 184 3472
MscI TGG'CCA
Cuts at: 1417 1417
Size: 3656
MslI CAynn'nnrTG
Cuts at: 1645 1772 1645
Size: 127 3529
MspA1I CmG'CkG
Cuts at: 767 1081 1441 2454 2699 767
Size: 314 360 1013 245 1724
Fragments arranged by size:
1724 1013 360 314 245
NaeI GCC'GGC
Cuts at: 130 1837 130
Size: 1707 1949
NarI GG'CG_CC
Cuts at: 1087 1335 1087
Size: 248 3408
NgoMIV G'CCGG_C
Cuts at: 128 1835 128
Size: 1707 1949
NheI G'CTAG_C
Cuts at: 321 321
Size: 3656
NotI GC'GGCC_GC
Cuts at: 145 145
Size: 3656
NspI r_CATG'y
Cuts at: 329 709 1209 2116 329
Size: 380 500 907 1869
Fragments arranged by size:
1869 907 500 380
PciI A'CATG_T
Cuts at: 2112 2112
Size: 3656
PfoI T'CCnGG_A
Cuts at: 461 497 461
Size: 36 3620
PmeI GTTT'AAAC
Cuts at: 655 655
Size: 3656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2795 2827 2795
Size: 32 3624
PsiI TTA'TAA
Cuts at: 644 644
Size: 3656
PstI C_TGCA'G
Cuts at: 621 703 1388 621
Size: 82 685 2889
Fragments arranged by size:
2889 685 82
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3656
PvuII CAG'CTG
Cuts at: 1081 1441 1081
Size: 360 3296
RsrII CG'GwC_CG
Cuts at: 1851 1851
Size: 3656
SacI G_AGCT'C
Cuts at: 670 670
Size: 3656
SalI G'TCGA_C
Cuts at: 693 693
Size: 3656
SapI GCTCTTCn'nnn_
Cuts at: 1679 1889 1679
Size: 210 3446
SbfI CC_TGCA'GG
Cuts at: 703 703
Size: 3656
ScaI AGT'ACT
Cuts at: 3173 3173
Size: 3656
SfcI C'TryA_G
Cuts at: 33 534 617 699 1384 2377 2568 33
Size: 501 83 82 685 993 191 1121
Fragments arranged by size:
1121 993 685 501 191 83 82
SfoI GGC'GCC
Cuts at: 1088 1336 1088
Size: 248 3408
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3656
SmaI CCC'GGG
Cuts at: 678 678
Size: 3656
SmlI C'TyrA_G
Cuts at: 202 811 1726 2218 2480 202
Size: 609 915 492 262 1378
Fragments arranged by size:
1378 915 609 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3656
SphI G_CATG'C
Cuts at: 709 1209 709
Size: 500 3156
StuI AGG'CCT
Cuts at: 412 412
Size: 3656
StyI C'CwwG_G
Cuts at: 790 1144 790
Size: 354 3302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1239 1372 1831 2040 1239
Size: 133 459 209 2855
Fragments arranged by size:
2855 459 209 133
TatI w'GTAC_w
Cuts at: 3171 3171
Size: 3656
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 3624
Tth111I GACn'n_nGTC
Cuts at: 1453 1453
Size: 3656
XbaI T'CTAG_A
Cuts at: 687 687
Size: 3656
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 169 1151 169
Size: 982 2674
XmaI C'CCGG_G
Cuts at: 676 676
Size: 3656
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NotI NspI PciI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI