Restriction map for pF1KB6546
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BanI        BanII       BbeI        BbrPI       BciVI       
BclI        BcnI        BfaI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MfeI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflII         154  CTTAAG               C^TTAAG
BamHI         588  GGATCC               G^GATCC
BbvCI         411  GCTGAGG              CCTCAGC(-5/-2)
BcgI          289  CGAGAGGTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          154  CTTAAG               C^TTAAG
BglII         621  AGATCT               A^GATCT
BmyI          770  GTGCCC               G[AGT]GC[ACT]^C
BsaBI         589  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         403  CTCCTC               GAGGAG(10/8)
BsgI          817  CTGCAC               GTGCAG(16/14)
BsmFI         252  GGGAC                GGGAC(10/14)
Bsp1286I      770  GTGCCC               G[AGT]GC[ACT]^C
BspHI          39  TCATGA               T^CATGA
BspMI         435  GCAGGT               ACCTGC(4/8)
Cac8I         660  GCATGC               GC[ACGT]^[ACGT]GC
DraI          450  TTTAAA               TTT^AAA
EcoNI         904  CCTTTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV          22  GATATC               GAT^ATC
HaeIII        273  GGCC                 GG^CC
MamI          589  GATCCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        409  CAGCTG               C[AC]G^C[GT]G
MwoI          274  GCCTATCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          507  CCATGG               C^CATGG
NdeI          131  CATATG               CA^TATG
NlaIV         588  GGATCC               GG[ACGT]^[ACGT]CC
PflMI         507  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         409  CAGCTG               CAG^CTG
RcaI           39  TCATGA               T^CATGA
SphI          660  GCATGC               GCATG^C
StuI          272  AGGCCT               AGG^CCT
SwaI          449  ATTTAAAT             ATTT^AAAT
Van91I        507  CCATGGTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          715  GTAC                 GT^AC
              989  GTAC                
BbvI          378  GCAGC                GCAGC(8/12)
              657  GCAGC               
BpmI           71  CTCCAG               CTGGAG(16/14)
              406  CTCCAG              
BsmAI         803  GAGAC                GTCTC(1/5)
             1075  GAGAC               
BssKI         910  CCAGG                ^CC[ACGT]GG
             1043  CCTGG               
BstNI         910  CCAGG                CC^[AT]GG
             1043  CCTGG               
DsaI          248  CCGTGG               C^C[GA][CT]GG
              507  CCATGG              
EcoRII        910  CCAGG                ^CC[AT]GG
             1043  CCTGG               
EcoT14I       507  CCATGG               C^C[AT][AT]GG
              795  CCTTGG              
EcoT22I       281  ATGCAT               ATGCA^T
              662  ATGCAT              
Fnu4HI        378  GCAGC                GC^[ACGT]GC
              657  GCAGC               
HphI          438  GGTGA                GGTGA(8/7)
              705  GGTGA               
ItaI          378  GCAGC                GC^[ACGT]GC
              657  GCAGC               
MvaI          910  CCAGG                CC^[AT]GG
             1043  CCTGG               
NsiI          281  ATGCAT               ATGCA^T
              662  ATGCAT              
NspI          660  GCATGC               [GA]CATG^[CT]
              991  ACATGC              
PleI           69  GACTC                GAGTC(4/5)
              708  GACTC               
RsaI          715  GTAC                 GT^AC
              989  GTAC                
SapI          318  GAAGAGC              GCTCTTC(1/4)
              612  GAAGAGC             
ScrFI         910  CCAGG                CC^[ACGT]GG
             1043  CCTGG               
SfaNI           4  GATGC                GCATC(5/9)
              782  GATGC               
SfcI          568  CTACAG               C^T[GA][CT]AG
              776  CTGTAG              
SmlI          154  CTTAAG               C^T[CT][GA]AG
              710  CTCAAG              
StyI          507  CCATGG               C^C[AT][AT]GG
              795  CCTTGG              
TaqI          225  TCGA                 T^CGA
              288  TCGA                
TseI          378  GCAGC                G^C[AT]GC
              657  GCAGC               
TthHB8I       225  TCGA                 T^CGA
              288  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BbsI        
BpuAI       BsaJI       BsiYI       BslI        BsrI        
BstF5I      BstYI       DdeI        DpnII       EarI        
FokI        HinfI       Ksp632I     MaeIII      MboI        
MboII       MflI        MnlI        MseI        NdeII       
NlaIII      Sau3AI      TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       XhoII       
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