Restriction map for pF1KB3103
 (Linear) (Six-base) MAPSORT of: KIBB3103.seq  Check: 8446  from: 1  to: 1650



KIBB3103.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     330    1650 
   Size:        330    1320
 
AccI GT'mk_AC

Cuts at:      0     506    1650 
   Size:        506    1144
 
AflII C'TTAA_G

Cuts at:      0     205    1650 
   Size:        205    1445
 
AleI CACnn'nnGTG

Cuts at:      0     985    1650 
   Size:        985     665
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1015    1383    1402    1650 
   Size:       1015     368      19     248

  Fragments arranged by size: 

                1015     368     248      19
 
ApoI r'AATT_y

Cuts at:      0     259     703     814    1650 
   Size:        259     444     111     836

  Fragments arranged by size: 

                 836     444     259     111
 
AvaI C'yCGr_G

Cuts at:      0    1248    1425    1650 
   Size:       1248     177     225

  Fragments arranged by size: 

                1248     225     177
 
BanI G'GyrC_C

Cuts at:      0     118     143    1650 
   Size:        118      25    1507

  Fragments arranged by size: 

                1507     118      25
 
BanII G_rGCy'C

Cuts at:      0     139    1122    1255    1331    1619    1650 
   Size:        139     983     133      76     288      31

  Fragments arranged by size: 

                 983     288     139     133      76      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     436    1012    1650 
   Size:        436     576     638

  Fragments arranged by size: 

                 638     576     436
 
BbvCI CC'TCA_GC

Cuts at:      0     172    1223    1650 
   Size:        172    1051     427

  Fragments arranged by size: 

                1051     427     172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     925     959    1650 
   Size:        925      34     691

  Fragments arranged by size: 

                 925     691      34
 
BclI T'GATC_A

Cuts at:      0     396    1650 
   Size:        396    1254
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1379    1399    1650 
   Size:       1379      20     251

  Fragments arranged by size: 

                1379     251      20
 
BglII A'GATC_T

Cuts at:      0    1529    1650 
   Size:       1529     121
 
BlpI GC'TnA_GC

Cuts at:      0    1291    1650 
   Size:       1291     359
 
Bme1580I G_kGCm'C

Cuts at:      0     146     741    1650 
   Size:        146     595     909

  Fragments arranged by size: 

                 909     595     146
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      72    1650 
   Size:         72    1578
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447    1192    1650 
   Size:        447     745     458

  Fragments arranged by size: 

                 745     458     447
 
Bpu10I CC'TnA_GC

Cuts at:      0     172    1073    1223    1650 
   Size:        172     901     150     427

  Fragments arranged by size: 

                 901     427     172     150
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      61    1650 
   Size:         61    1589
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1318    1348    1650 
   Size:       1318      30     302

  Fragments arranged by size: 

                1318     302      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1189    1249    1650 
   Size:       1189      60     401

  Fragments arranged by size: 

                1189     401      60
 
BseYI C'CCAG_C

Cuts at:      0     746     893    1121    1623    1650 
   Size:        746     147     228     502      27

  Fragments arranged by size: 

                 746     502     228     147      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     511     733     955    1650 
   Size:        151     360     222     222     695

  Fragments arranged by size: 

                 695     360     222     222     151
 
BsiEI CG_ry'CG

Cuts at:      0     501    1650 
   Size:        501    1149
 
BsmI GAATG_Cn'

Cuts at:      0     963    1650 
   Size:        963     687
 
Bsp1286I G_dGCh'C

Cuts at:      0     139     146     741    1122    1255    1331    1619    1650 
   Size:        139       7     595     381     133      76     288      31

  Fragments arranged by size: 

                 595     381     288     139     133      76      31       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     330    1650 
   Size:        330    1320
 
BsrBI CCG'CTC

Cuts at:      0    1140    1650 
   Size:       1140     510
 
BsrFI r'CCGG_y

Cuts at:      0    1607    1650 
   Size:       1607      43
 
BsrGI T'GTAC_A

Cuts at:      0    1334    1650 
   Size:       1334     316
 
BssSI C'ACGA_G

Cuts at:      0     553     664     886    1650 
   Size:        553     111     222     764

  Fragments arranged by size: 

                 764     553     222     111
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     250    1635    1650 
   Size:        250    1385      15

  Fragments arranged by size: 

                1385     250      15
 
BstYI r'GATC_y

Cuts at:      0    1529    1650 
   Size:       1529     121
 
BtgI C'CryG_G

Cuts at:      0     243     354    1650 
   Size:        243     111    1296

  Fragments arranged by size: 

                1296     243     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      18    1535    1650 
   Size:         18    1517     115

  Fragments arranged by size: 

                1517     115      18
 
BtsI GCAGTG_nn'

Cuts at:      0     330    1650 
   Size:        330    1320
 
DraIII CAC_nnn'GTG

Cuts at:      0      27    1650 
   Size:         27    1623
 
EaeI y'GGCC_r

Cuts at:      0     300     878    1130    1650 
   Size:        300     578     252     520

  Fragments arranged by size: 

                 578     520     300     252
 
EarI CTCTTCn'nnn_

Cuts at:      0    1148    1624    1650 
   Size:       1148     476      26

  Fragments arranged by size: 

                1148     476      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1113    1120    1126    1650 
   Size:       1113       7       6     524

  Fragments arranged by size: 

                1113     524       7       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     447    1192    1650 
   Size:        447     745     458

  Fragments arranged by size: 

                 745     458     447
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1072    1650 
   Size:       1072     578
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1303    1380    1422    1650 
   Size:       1303      77      42     228

  Fragments arranged by size: 

                1303     228      77      42
 
HaeII r_GCGC'y

Cuts at:      0     629    1650 
   Size:        629    1021
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1516    1548    1650 
   Size:       1516      32     102

  Fragments arranged by size: 

                1516     102      32
 
HincII GTy'rAC

Cuts at:      0     965    1650 
   Size:        965     685
 
HindIII A'AGCT_T

Cuts at:      0       8     236    1650 
   Size:          8     228    1414

  Fragments arranged by size: 

                1414     228       8
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1650 
   Size:        809     841
 
MscI TGG'CCA

Cuts at:      0     880    1132    1650 
   Size:        880     252     518

  Fragments arranged by size: 

                 880     518     252
 
MslI CAynn'nnrTG

Cuts at:      0     248     985    1567    1650 
   Size:        248     737     582      83

  Fragments arranged by size: 

                 737     582     248      83
 
MspA1I CmG'CkG

Cuts at:      0     295     771     847    1125    1351    1650 
   Size:        295     476      76     278     226     299

  Fragments arranged by size: 

                 476     299     295     278     226      76
 
NcoI C'CATG_G

Cuts at:      0     243    1650 
   Size:        243    1407
 
NspI r_CATG'y

Cuts at:      0     445    1650 
   Size:        445    1205
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1128    1650 
   Size:       1128     522
 
PsiI TTA'TAA

Cuts at:      0     794    1650 
   Size:        794     856
 
PspXI vC'TCGA_Gb

Cuts at:      0    1248    1650 
   Size:       1248     402
 
PvuII CAG'CTG

Cuts at:      0     295     771    1650 
   Size:        295     476     879

  Fragments arranged by size: 

                 879     476     295
 
RsrII CG'GwC_CG

Cuts at:      0     351    1650 
   Size:        351    1299
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1624    1650 
   Size:       1624      26
 
SfcI C'TryA_G

Cuts at:      0     407    1296    1650 
   Size:        407     889     354

  Fragments arranged by size: 

                 889     407     354
 
SmaI CCC'GGG

Cuts at:      0    1427    1650 
   Size:       1427     223
 
SmlI C'TyrA_G

Cuts at:      0     205    1248    1650 
   Size:        205    1043     402

  Fragments arranged by size: 

                1043     402     205
 
SphI G_CATG'C

Cuts at:      0     445    1650 
   Size:        445    1205
 
StyI C'CwwG_G

Cuts at:      0     243     741    1133    1650 
   Size:        243     498     392     517

  Fragments arranged by size: 

                 517     498     392     243
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      85     293     339     515     997    1650 
   Size:         85     208      46     176     482     653

  Fragments arranged by size: 

                 653     482     208     176      85      46
 
TatI w'GTAC_w

Cuts at:      0    1334    1650 
   Size:       1334     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45     249     281    1650 
   Size:         13      32     204      32    1369

  Fragments arranged by size: 

                1369     204      32      32      13
 
Tth111I GACn'n_nGTC

Cuts at:      0     349    1650 
   Size:        349    1301
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1167    1650 
   Size:       1167     483
 
XhoI C'TCGA_G

Cuts at:      0    1248    1650 
   Size:       1248     402
 
XmaI C'CCGG_G

Cuts at:      0    1425    1650 
   Size:       1425     225
 

 Enzymes that do cut:

     AarI     AccI    AflII     AleI    AlwNI     ApoI     AvaI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaXI    BseRI    BseYI
     BsgI    BsiEI     BsmI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI
    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
  HindIII     MmeI     MscI     MslI   MspA1I     NcoI     NspI    PflMI
     PsiI    PspXI    PvuII    RsrII     SapI     SfcI     SmaI     SmlI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BciVI    BfrBI    BmgBI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PsrI     PstI     PvuI     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     XbaI     XmnI     ZraI
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