Restriction map for pF1KB7015
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AflII       
AgeI        AlwI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AvrII       BamHI       
BciVI       BfrI        BlnI        BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BssHII      Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     ClaI        DraI        
DraIII      DrdI        EcoRI       EcoRV       EcoT22I     
FseI        HincII      HindII      HindIII     HpaI        
KpnI        MfeI        MroI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        SalI        SfuI        
SgrAI       SnaBI       SpeI        SspI        SwaI        
TfiI        Tru9I       XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccI         2008  GTCTAC               GT^[AC][GT]AC
AflIII       1283  ACGCGT               A^C[GA][CT]GT
Aor51HI       740  AGCGCT               AGC^GCT
BbrPI         868  CACGTG               CAC^GTG
BcgI         1371  CGAGCTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2174  TGATCA               T^GATCA
BfaI          961  CTAG                 C^TAG
BlpI          174  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      174  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1890  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          291  GAATGC               GAATGC(1/-1)
BsrDI        1862  GCAATG               GCAATG(2/0)
BssSI         301  CACGAG               CACGAG(-5/-1)
CelII         174  GCTGAGC              GC^T[ACGT]AGC
CpoI          662  CGGACCG              CG^G[AT]CCG
EarI         2107  CTCTTC               CTCTTC(1/4)
Eco47III      740  AGCGCT               AGC^GCT
FbaI         2174  TGATCA               T^GATCA
Ksp632I      2107  CTCTTC               CTCTTC(1/4)
MaeI          961  CTAG                 C^TAG
MamI         1890  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1283  ACGCGT               A^CGCGT
NcoI          203  CCATGG               C^CATGG
NspI         2193  GCATGC               [GA]CATG^[CT]
PaeR7I       1369  CTCGAG               C^TCGAG
PmaCI         868  CACGTG               CAC^GTG
PmlI          868  CACGTG               CAC^GTG
PshAI        1262  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         662  CGGACCG              CG^G[AT]CCG
SapI         2106  GCTCTTC              GCTCTTC(1/4)
SbfI          762  CCTGCAGG             CCTGCA^GG
SphI         2193  GCATGC               GCATG^C
Sse8387I      762  CCTGCAGG             CCTGCA^GG
Tsp509I      1027  AATT                 ^AATT
XhoI         1369  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AatII        2026  GACGTC               GACGT^C
             2262  GACGTC              
Alw44I       1231  GTGCAC               G^TGCAC
             1924  GTGCAC              
ApaLI        1231  GTGCAC               G^TGCAC
             1924  GTGCAC              
AspI         2002  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2245  GACCGTGTC           
AviII        1193  TGCGCA               TGC^GCA
             1313  TGCGCA              
BbvCI        1128  CCTCAGC              CCTCAGC(-5/-2)
              421  GCTGAGG             
BglII        1238  AGATCT               A^GATCT
             2057  AGATCT              
BsaAI         868  CACGTG               [CT]AC^GT[GA]
             1654  TACGTG              
BsgI         1632  CTGCAC               GTGCAG(16/14)
             2154  CTGCAC              
BsiEI         500  CGGCCG               CG[GA][CT]^CG
             1959  CGGCCG              
BsmAI        1360  GTCTC                GTCTC(1/5)
             1401  GTCTC               
Bsp1407I      830  TGTACA               T^GTACA
             2168  TGTACA              
BsrGI         830  TGTACA               T^GTACA
             2168  TGTACA              
BstEII       1681  GGTAACC              G^GT[ACGT]ACC
             1872  GGTGACC             
BstPI        1681  GGTAACC              G^GT[ACGT]ACC
             1872  GGTGACC             
BstYI        1238  AGATCT               [GA]^GATC[CT]
             2057  AGATCT              
EagI          500  CGGCCG               C^GGCCG
             1959  CGGCCG              
EclXI         500  CGGCCG               C^GGCCG
             1959  CGGCCG              
Eco52I        500  CGGCCG               C^GGCCG
             1959  CGGCCG              
EcoNI        1431  CCTTGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2204  CCTCCCAGAGG         
EcoO65I      1681  GGTAACC              G^GT[ACGT]ACC
             1872  GGTGACC             
FspI         1193  TGCGCA               TGC^GCA
             1313  TGCGCA              
KspI          585  CCGCGG               CCGC^GG
              666  CCGCGG              
MflI         1238  AGATCT               [GA]^GATC[CT]
             2057  AGATCT              
PflFI        2002  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2245  GACCGTGTC           
PstI          280  CTGCAG               CTGCA^G
              763  CTGCAG              
PvuII         321  CAGCTG               CAG^CTG
             2227  CAGCTG              
SacII         585  CCGCGG               CCGC^GG
              666  CCGCGG              
ScaI         1817  AGTACT               AGT^ACT
             2306  AGTACT              
SfiI           42  GGCCATCGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              492  GGCCGTGCCGGCC       
SspBI         830  TGTACA               T^GTACA
             2168  TGTACA              
StuI         1212  AGGCCT               AGG^CCT
             1991  AGGCCT              
Tth111I      2002  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2245  GACCGTGTC           
XcmI         1205  CCAAGAAAGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1298  CCAACACACCACTGG     
XhoII        1238  AGATCT               [GA]^GATC[CT]
             2057  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AhdI        
AluI        AlwNI       AocI        ApaI        AspEI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbsI        BbvI        BcnI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
KasI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PpuMI       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SexAI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com