Restriction map for pF1KB5550
 (Linear) (Six-base) MAPSORT of: KIBB5550.seq  Check: 6385  from: 1  to: 1962



KIBB5550.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0    1009    1962 
   Size:       1009     953
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     446    1559    1625    1784    1962 
   Size:        230     216    1113      66     159     178

  Fragments arranged by size: 

                1113     230     216     178     159      66
 
AleI CACnn'nnGTG

Cuts at:      0    1324    1962 
   Size:       1324     638
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     322     354    1962 
   Size:        322      32    1608

  Fragments arranged by size: 

                1608     322      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1119    1363    1962 
   Size:       1119     244     599

  Fragments arranged by size: 

                1119     599     244
 
ApaI G_GGCC'C

Cuts at:      0     894    1962 
   Size:        894    1068
 
ApoI r'AATT_y

Cuts at:      0     431     976    1100    1457    1962 
   Size:        431     545     124     357     505

  Fragments arranged by size: 

                 545     505     431     357     124
 
AvaI C'yCGr_G

Cuts at:      0     262     968    1962 
   Size:        262     706     994

  Fragments arranged by size: 

                 994     706     262
 
BanI G'GyrC_C

Cuts at:      0    1326    1962 
   Size:       1326     636
 
BanII G_rGCy'C

Cuts at:      0     894     989    1962 
   Size:        894      95     973

  Fragments arranged by size: 

                 973     894      95
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     714     773     823     890     953    1547    1658    1847 
   Size:        714      59      50      67      63     594     111     189

Cuts at:   1847    1962 
   Size:        115

  Fragments arranged by size: 

                 714     594     189     115     111      67      63      59
                  50
 
BbvCI CC'TCA_GC

Cuts at:      0     470    1646    1962 
   Size:        470    1176     316

  Fragments arranged by size: 

                1176     470     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      12      46      72     106    1962 
   Size:         12      34      26      34    1856

  Fragments arranged by size: 

                1856      34      34      26      12
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1932    1962 
   Size:       1932      30
 
BclI T'GATC_A

Cuts at:      0    1221    1962 
   Size:       1221     741
 
BglI GCCn_nnn'nGGC

Cuts at:      0     889    1962 
   Size:        889    1073
 
BglII A'GATC_T

Cuts at:      0    1346    1962 
   Size:       1346     616
 
BlpI GC'TnA_GC

Cuts at:      0      37    1962 
   Size:         37    1925
 
Bme1580I G_kGCm'C

Cuts at:      0      85     894    1962 
   Size:         85     809    1068

  Fragments arranged by size: 

                1068     809      85
 
BmgBI CAC'GTC

Cuts at:      0      78    1962 
   Size:         78    1884
 
Bpu10I CC'TnA_GC

Cuts at:      0     470    1646    1962 
   Size:        470    1176     316

  Fragments arranged by size: 

                1176     470     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1648    1962 
   Size:       1648     314
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     952    1768    1962 
   Size:        952     816     194

  Fragments arranged by size: 

                 952     816     194
 
BsaAI yAC'GTr

Cuts at:      0     305    1962 
   Size:        305    1657
 
BsaBI GATnn'nnATC

Cuts at:      0     752    1573    1962 
   Size:        752     821     389

  Fragments arranged by size: 

                 821     752     389
 
BsaHI Gr'CG_yC

Cuts at:      0     281    1962 
   Size:        281    1681
 
BsaWI w'CCGG_w

Cuts at:      0    1004    1962 
   Size:       1004     958
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     405     435    1962 
   Size:        405      30    1527

  Fragments arranged by size: 

                1527     405      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70      73    1460    1962 
   Size:         70       3    1387     502

  Fragments arranged by size: 

                1387     502      70       3
 
BseYI C'CCAG_C

Cuts at:      0    1743    1962 
   Size:       1743     219
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343     874    1931    1962 
   Size:        343     531    1057      31

  Fragments arranged by size: 

                1057     531     343      31
 
BsiEI CG_ry'CG

Cuts at:      0     144     300    1962 
   Size:        144     156    1662

  Fragments arranged by size: 

                1662     156     144
 
BsiHKAI G_wGCw'C

Cuts at:      0     989    1214    1962 
   Size:        989     225     748

  Fragments arranged by size: 

                 989     748     225
 
Bsp1286I G_dGCh'C

Cuts at:      0      85     894     989    1214    1962 
   Size:         85     809      95     225     748

  Fragments arranged by size: 

                 809     748     225      95      85
 
BspHI T'CATG_A

Cuts at:      0    1295    1962 
   Size:       1295     667
 
BsrGI T'GTAC_A

Cuts at:      0     806    1962 
   Size:        806    1156
 
BssSI C'ACGA_G

Cuts at:      0    1472    1962 
   Size:       1472     490
 
BstBI TT'CG_AA

Cuts at:      0     725    1962 
   Size:        725    1237
 
BstEII G'GTnAC_C

Cuts at:      0     492    1234    1962 
   Size:        492     742     728

  Fragments arranged by size: 

                 742     728     492
 
BstYI r'GATC_y

Cuts at:      0     996    1346    1962 
   Size:        996     350     616

  Fragments arranged by size: 

                 996     616     350
 
BtgI C'CryG_G

Cuts at:      0     440    1155    1778    1962 
   Size:        440     715     623     184

  Fragments arranged by size: 

                 715     623     440     184
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      37     141     245    1962 
   Size:         37     104     104    1717

  Fragments arranged by size: 

                1717     104     104      37
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     718     781    1962 
   Size:        718      63    1181

  Fragments arranged by size: 

                1181     718      63
 
EaeI y'GGCC_r

Cuts at:      0      17     141     297     734    1962 
   Size:         17     124     156     437    1228

  Fragments arranged by size: 

                1228     437     156     124      17
 
EagI C'GGCC_G

Cuts at:      0     141     297    1962 
   Size:        141     156    1665

  Fragments arranged by size: 

                1665     156     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     704     977    1928    1962 
   Size:        704     273     951      34

  Fragments arranged by size: 

                 951     704     273      34
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     695    1962 
   Size:        695    1267
 
EcoICRI GAG'CTC

Cuts at:      0     987    1962 
   Size:        987     975
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     230     446    1559    1625    1784    1962 
   Size:        230     216    1113      66     159     178

  Fragments arranged by size: 

                1113     230     216     178     159      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     891    1479    1915    1962 
   Size:        891     588     436      47

  Fragments arranged by size: 

                 891     588     436      47
 
EcoRI G'AATT_C

Cuts at:      0    1100    1457    1962 
   Size:       1100     357     505

  Fragments arranged by size: 

                1100     505     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     405     437     951     983    1962 
   Size:        405      32     514      32     979

  Fragments arranged by size: 

                 979     514     405      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1016    1673    1962 
   Size:        281     735     657     289

  Fragments arranged by size: 

                 735     657     289     281
 
MscI TGG'CCA

Cuts at:      0     736    1962 
   Size:        736    1226
 
MslI CAynn'nnrTG

Cuts at:      0     454     661     758    1324    1826    1962 
   Size:        454     207      97     566     502     136

  Fragments arranged by size: 

                 566     502     454     207     136      97
 
MspA1I CmG'CkG

Cuts at:      0     469     613    1537    1962 
   Size:        469     144     924     425

  Fragments arranged by size: 

                 924     469     425     144
 
NcoI C'CATG_G

Cuts at:      0     440    1778    1962 
   Size:        440    1338     184

  Fragments arranged by size: 

                1338     440     184
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1156    1962 
   Size:       1156     806
 
PmlI CAC'GTG

Cuts at:      0     305    1962 
   Size:        305    1657
 
PpuMI rG'GwC_Cy

Cuts at:      0    1479    1962 
   Size:       1479     483
 
PspOMI G'GGCC_C

Cuts at:      0     890    1962 
   Size:        890    1072
 
PvuII CAG'CTG

Cuts at:      0     613    1537    1962 
   Size:        613     924     425

  Fragments arranged by size: 

                 924     613     425
 
SacI G_AGCT'C

Cuts at:      0     989    1962 
   Size:        989     973
 
SanDI GG'GwC_CC

Cuts at:      0    1479    1962 
   Size:       1479     483
 
SapI GCTCTTCn'nnn_

Cuts at:      0     977    1962 
   Size:        977     985
 
SexAI A'CCwGG_T

Cuts at:      0    1238    1962 
   Size:       1238     724
 
SfcI C'TryA_G

Cuts at:      0    1178    1355    1790    1962 
   Size:       1178     177     435     172

  Fragments arranged by size: 

                1178     435     177     172
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     889    1962 
   Size:        889    1073
 
SmlI C'TyrA_G

Cuts at:      0    1663    1962 
   Size:       1663     299
 
StyI C'CwwG_G

Cuts at:      0     440    1312    1778    1962 
   Size:        440     872     466     184

  Fragments arranged by size: 

                 872     466     440     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     664     748    1962 
   Size:        664      84    1214

  Fragments arranged by size: 

                1214     664      84
 
TatI w'GTAC_w

Cuts at:      0     806    1962 
   Size:        806    1156
 
XmnI GAAnn'nnTTC

Cuts at:      0    1076    1877    1962 
   Size:       1076     801      85

  Fragments arranged by size: 

                1076     801      85
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AloI    AlwNI     ApaI     ApoI     AvaI
     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I    BmgBI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BsrGI    BssSI    BstBI   BstEII    BstYI
     BtgI    BtgZI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI    Hin4I     MmeI     MscI     MslI   MspA1I
     NcoI    PflMI     PmlI    PpuMI   PspOMI    PvuII     SacI    SanDI
     SapI    SexAI     SfcI     SfiI     SmlI     StyI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BfrBI     BmrI     BmtI     BplI     BpmI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
   BstAPI    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PpiI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI     SbfI
     ScaI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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