Restriction map for pF1KB7591
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HindIII     HpaI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvnI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp509I     Tth111I     XbaI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        581  GGTACC               G^GTACC
Aor51HI       383  AGCGCT               AGC^GCT
ApaI          290  GGGCCC               GGGCC^C
Asp718I       581  GGTACC               G^GTACC
AspHI         116  GTGCTC               G[AT]GC[AT]^C
AvaII          89  GGACC                G^G[AT]CC
BanII         290  GGGCCC               G[GA]GC[CT]^C
BbvCI         648  CCTCAGC              CCTCAGC(-5/-2)
BlpI          364  GCTAAGC              GC^T[ACGT]AGC
BpmI          250  CTGGAG               CTGGAG(16/14)
Bpu1102I      364  GCTAAGC              GC^T[ACGT]AGC
BsiHKAI       116  GTGCTC               G[AT]GC[AT]^C
BspMI         493  ACCTGC               ACCTGC(4/8)
BsrFI         607  GCCGGC               [GA]^CCGG[CT]
BstF5I        662  CATCC                GGATG(2/0)
BstXI          98  CCACCACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         364  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        607  GCCGGC               [GA]^CCGG[CT]
DpnII         158  GATC                 ^GATC
DraII          88  GGGACCC              [GA]G^G[ACGT]CC[CT]
DsaI          101  CCACGG               C^C[GA][CT]GG
Eco47III      383  AGCGCT               AGC^GCT
EcoO109I       88  GGGACCC              [GA]G^G[ACGT]CC[CT]
FokI          662  CATCC                GGATG(9/13)
HphI          504  TCACC                GGTGA(8/7)
KpnI          581  GGTACC               GGTAC^C
MboI          158  GATC                 ^GATC
MslI           99  CACCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        247  CAGCTG               C[AC]G^C[GT]G
NaeI          607  GCCGGC               GCC^GGC
NdeII         158  GATC                 ^GATC
NgoMIV        607  GCCGGC               G^CCGGC
PaeR7I        111  CTCGAG               C^TCGAG
PflMI         665  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          88  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          126  CTGCAG               CTGCA^G
PvuII         247  CAGCTG               CAG^CTG
Sau3AI        158  GATC                 ^GATC
SmaI          308  CCCGGG               CCC^GGG
TaqI          112  TCGA                 T^CGA
TfiI          335  GAATC                G^A[AT]TC
TthHB8I       112  TCGA                 T^CGA
Van91I        665  CCAAGTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          661  CCATCCAAGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          111  CTCGAG               C^TCGAG
XmaCI         308  CCCGGG               C^CCGGG
XmaI          308  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          276  GTAC                 GT^AC
              582  GTAC                
AlwNI         397  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              599  CAGCCCCTG           
BmyI          116  GTGCTC               G[AGT]GC[ACT]^C
              290  GGGCCC              
BsgI          143  GTGCAG               GTGCAG(16/14)
              235  GTGCAG              
BsmAI          72  GAGAC                GTCTC(1/5)
              414  GTCTC               
BsmFI          88  GGGAC                GGGAC(10/14)
              311  GGGAC               
Bsp1286I      116  GTGCTC               G[AGT]GC[ACT]^C
              290  GGGCCC              
EaeI           80  CGGCCA               [CT]^GGCC[GA]
              609  CGGCCA              
FauI          171  CCCGC                CCCGC(4/6)
              195  CCCGC               
MaeIII        490  GTGAC                ^GT[ACGT]AC
              561  GTCAC               
NlaIII        517  CATG                 CATG^
              639  CATG                
RsaI          276  GTAC                 GT^AC
              582  GTAC                
SfcI          126  CTGCAG               C^T[GA][CT]AG
              510  CTACAG              
SmlI          111  CTCGAG               C^T[CT][GA]AG
              655  CTTGAG              
Tsp45I        490  GTGAC                ^GT[GC]AC
              561  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcyI        AluI        AvaI        BanI        
BbeI        BbvI        BcnI        BsaHI       BsaJI       
BsiYI       BslI        BsoBI       BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
EcoNI       EcoRII      Fnu4HI      HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        KasI        MnlI        MspI        
MvaI        MwoI        NarI        NciI        NlaIV       
PleI        Sau96I      ScrFI       SfoI        TseI        
TspRI       
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