Restriction map for pF1KB7591
 (Linear) (Six-base) MAPSORT of: KIBB7591.seq  Check: 3109  from: 1  to: 678



KIBB7591.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

Acc65I G'GTAC_C

Cuts at:      0     581     678 
   Size:        581      97
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     379     678 
   Size:        210     169     299

  Fragments arranged by size: 

                 299     210     169
 
AfeI AGC'GCT

Cuts at:      0     385     678 
   Size:        385     293
 
AleI CACnn'nnGTG

Cuts at:      0     103     678 
   Size:        103     575
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      79     678 
   Size:         79     599
 
AlwNI CAG_nnn'CTG

Cuts at:      0     402     604     678 
   Size:        402     202      74

  Fragments arranged by size: 

                 402     202      74
 
ApaI G_GGCC'C

Cuts at:      0     294     678 
   Size:        294     384
 
AvaI C'yCGr_G

Cuts at:      0     111     293     308     678 
   Size:        111     182      15     370

  Fragments arranged by size: 

                 370     182     111      15
 
BanI G'GyrC_C

Cuts at:      0      17     206     547     581     678 
   Size:         17     189     341      34      97

  Fragments arranged by size: 

                 341     189      97      34      17
 
BanII G_rGCy'C

Cuts at:      0     294     678 
   Size:        294     384
 
BbeI G_GCGC'C

Cuts at:      0      21     210     551     678 
   Size:         21     189     341     127

  Fragments arranged by size: 

                 341     189     127      21
 
BbvCI CC'TCA_GC

Cuts at:      0     649     678 
   Size:        649      29
 
BlpI GC'TnA_GC

Cuts at:      0     365     678 
   Size:        365     313
 
Bme1580I G_kGCm'C

Cuts at:      0     294     678 
   Size:        294     384
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      95     226     678 
   Size:         95     131     452

  Fragments arranged by size: 

                 452     131      95
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     678 
   Size:        271     407
 
Bpu10I CC'TnA_GC

Cuts at:      0     649     678 
   Size:        649      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     678 
   Size:        676       2
 
BsaHI Gr'CG_yC

Cuts at:      0      18     207     548     678 
   Size:         18     189     341     130

  Fragments arranged by size: 

                 341     189     130      18
 
BseYI C'CCAG_C

Cuts at:      0     530     597     678 
   Size:        530      67      81

  Fragments arranged by size: 

                 530      81      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     256     678 
   Size:        164      92     422

  Fragments arranged by size: 

                 422     164      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     120     678 
   Size:        120     558
 
Bsp1286I G_dGCh'C

Cuts at:      0     120     294     678 
   Size:        120     174     384

  Fragments arranged by size: 

                 384     174     120
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     502     678 
   Size:        502     176
 
BsrFI r'CCGG_y

Cuts at:      0     607     678 
   Size:        607      71
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     105     678 
   Size:        105     573
 
BtgI C'CryG_G

Cuts at:      0     101     678 
   Size:        101     577
 
BtsI GCAGTG_nn'

Cuts at:      0     388     449     678 
   Size:        388      61     229

  Fragments arranged by size: 

                 388     229      61
 
EaeI y'GGCC_r

Cuts at:      0      80     609     678 
   Size:         80     529      69

  Fragments arranged by size: 

                 529      80      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     271     379     678 
   Size:        210      61     108     299

  Fragments arranged by size: 

                 299     210     108      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     129     143     264     325     678 
   Size:        129      14     121      61     353

  Fragments arranged by size: 

                 353     129     121      61      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     678 
   Size:         89     589
 
HaeII r_GCGC'y

Cuts at:      0      21     210     387     551     678 
   Size:         21     189     177     164     127

  Fragments arranged by size: 

                 189     177     164     127      21
 
KasI G'GCGC_C

Cuts at:      0      17     206     547     678 
   Size:         17     189     341     131

  Fragments arranged by size: 

                 341     189     131      17
 
KpnI G_GTAC'C

Cuts at:      0     585     678 
   Size:        585      93
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     678 
   Size:        221     457
 
MslI CAynn'nnrTG

Cuts at:      0     103     678 
   Size:        103     575
 
MspA1I CmG'CkG

Cuts at:      0     249     678 
   Size:        249     429
 
NaeI GCC'GGC

Cuts at:      0     609     678 
   Size:        609      69
 
NarI GG'CG_CC

Cuts at:      0      18     207     548     678 
   Size:         18     189     341     130

  Fragments arranged by size: 

                 341     189     130      18
 
NgoMIV G'CCGG_C

Cuts at:      0     607     678 
   Size:        607      71
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     671     678 
   Size:        671       7
 
PfoI T'CCnGG_A

Cuts at:      0     670     678 
   Size:        670       8
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     678 
   Size:         89     589
 
PspOMI G'GGCC_C

Cuts at:      0     290     678 
   Size:        290     388
 
PspXI vC'TCGA_Gb

Cuts at:      0     111     678 
   Size:        111     567
 
PstI C_TGCA'G

Cuts at:      0     130     678 
   Size:        130     548
 
PvuII CAG'CTG

Cuts at:      0     249     678 
   Size:        249     429
 
SanDI GG'GwC_CC

Cuts at:      0      89     678 
   Size:         89     589
 
SfcI C'TryA_G

Cuts at:      0     126     510     678 
   Size:        126     384     168

  Fragments arranged by size: 

                 384     168     126
 
SfoI GGC'GCC

Cuts at:      0      19     208     549     678 
   Size:         19     189     341     129

  Fragments arranged by size: 

                 341     189     129      19
 
SmaI CCC'GGG

Cuts at:      0     310     678 
   Size:        310     368
 
SmlI C'TyrA_G

Cuts at:      0     111     655     678 
   Size:        111     544      23

  Fragments arranged by size: 

                 544     111      23
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     611     678 
   Size:        611      67
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     668     678 
   Size:        668      10
 
XhoI C'TCGA_G

Cuts at:      0     111     678 
   Size:        111     567
 
XmaI C'CCGG_G

Cuts at:      0     308     678 
   Size:        308     370
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI     ApaI     AvaI
     BanI    BanII     BbeI    BbvCI     BlpI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI    BsaHI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrFI    BstXI     BtgI     BtsI     EaeI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     KpnI     MmeI     MslI   MspA1I     NaeI     NarI
   NgoMIV    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII
    SanDI     SfcI     SfoI     SmaI     SmlI    TaqII     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     MfeI     MluI
     MscI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XmnI     ZraI
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