Restriction map for pF1KE0098
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BalI        BbrPI       
BbsI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsiEI       BsiWI       Bsp1407I    
BspDI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HpaI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MscI        MseI        MslI        
MunI        MvnI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccIII        438  TCCGGA               T^CCGGA
AlwNI          25  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          646  GGGCCC               GGGCC^C
AviII        1068  TGCGCA               TGC^GCA
BamHI         394  GGATCC               G^GATCC
BbeI          853  GGCGCC               GGCGC^C
BbvCI         205  GCTGAGG              CCTCAGC(-5/-2)
BcgI          961  GCAGCAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          848  CTAG                 C^TAG
BglII         731  AGATCT               A^GATCT
BsaWI         438  TCCGGA               [AT]^CCGG[AT]
BseAI         438  TCCGGA               T^CCGGA
BsgI           97  GTGCAG               GTGCAG(16/14)
BsmBI         797  GAGACG               CGTCTC(1/5)
BsmI          434  GCATTC               GAATGC(1/-1)
BspEI         438  TCCGGA               T^CCGGA
BsrDI         737  GCAATG               GCAATG(2/0)
BsrFI         785  GCCGGC               [GA]^CCGG[CT]
BstAPI         24  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        785  GCCGGC               [GA]^CCGG[CT]
EaeI          806  TGGCCG               [CT]^GGCC[GA]
FspI         1068  TGCGCA               TGC^GCA
HaeII         853  GGCGCC               [GA]GCGC^[CT]
HgaI          287  GCGTC                GACGC(5/10)
HincII        748  GTCAAC               GT[CT]^[GA]AC
HindII        748  GTCAAC               GT[CT]^[GA]AC
HindIII       656  AAGCTT               A^AGCTT
KasI          853  GGCGCC               G^GCGCC
MaeI          848  CTAG                 C^TAG
MroI          438  TCCGGA               T^CCGGA
NaeI          785  GCCGGC               GCC^GGC
NarI          853  GGCGCC               GG^CGCC
NgoMIV        785  GCCGGC               G^CCGGC
PaeR7I         43  CTCGAG               C^TCGAG
PflMI         328  CCAACAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         129  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          944  CTGCAG               CTGCA^G
SfoI          853  GGCGCC               GGC^GCC
SmaI          684  CCCGGG               CCC^GGG
TaqI           44  TCGA                 T^CGA
Tsp45I        836  GTCAC                ^GT[GC]AC
TthHB8I        44  TCGA                 T^CGA
Van91I        328  CCAACAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI           43  CTCGAG               C^TCGAG
XmaCI         684  CCCGGG               C^CCGGG
XmaI          684  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          286  GGCGTC               G[GA]^CG[CT]C
              853  GGCGCC              
AfaI          605  GTAC                 GT^AC
             1095  GTAC                
AspHI         109  GTGCTC               G[AT]GC[AT]^C
              548  GTGCTC              
BanI          791  GGCACC               G^G[CT][GA]CC
              853  GGCGCC              
BanII         640  GGGCTC               G[GA]GC[CT]^C
              646  GGGCCC              
BsaHI         286  GGCGTC               G[GA]^CG[CT]C
              853  GGCGCC              
BseRI         517  GAGGAG               GAGGAG(10/8)
              520  GAGGAG              
BsiHKAI       109  GTGCTC               G[AT]GC[AT]^C
              548  GTGCTC              
BsmAI         442  GAGAC                GTCTC(1/5)
              797  GAGAC               
DraII         129  GGGACCC              [GA]G^G[ACGT]CC[CT]
              672  GGGGCCT             
DsaI           86  CCATGG               C^C[GA][CT]GG
              365  CCATGG              
EcoO109I      129  GGGACCC              [GA]G^G[ACGT]CC[CT]
              672  GGGGCCT             
FauI          868  GCGGG                CCCGC(4/6)
              910  GCGGG               
Hin1I         286  GGCGTC               G[GA]^CG[CT]C
              853  GGCGCC              
HinfI         242  GAGTC                G^A[ACGT]TC
              477  GACTC               
MaeIII        457  GTAAC                ^GT[ACGT]AC
              836  GTCAC               
MspA1I        956  CAGCGG               C[AC]G^C[GT]G
              973  CCGCTG              
NcoI           86  CCATGG               C^CATGG
              365  CCATGG              
NlaIII         87  CATG                 CATG^
              366  CATG                
PleI          477  GACTC                GAGTC(4/5)
              242  GAGTC               
RsaI          605  GTAC                 GT^AC
             1095  GTAC                
SfcI           78  CTACAG               C^T[GA][CT]AG
              944  CTGCAG              
Tsp509I       190  AATT                 ^AATT
              621  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIV       Sau3AI      
Sau96I      ScrFI       SmlI        StyI        TseI        
TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com