Restriction map for pF1KE0098
 (Linear) (Six-base) MAPSORT of: KIEE0098.seq  Check: 4143  from: 1  to: 1098



KIEE0098.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54    1098 
   Size:         54    1044
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     562     594    1098 
   Size:        562      32     504

  Fragments arranged by size: 

                 562     504      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30    1098 
   Size:         30    1068
 
ApaI G_GGCC'C

Cuts at:      0     650    1098 
   Size:        650     448
 
AvaI C'yCGr_G

Cuts at:      0      43     643     684    1098 
   Size:         43     600      41     414

  Fragments arranged by size: 

                 600     414      43      41
 
BamHI G'GATC_C

Cuts at:      0     394    1098 
   Size:        394     704
 
BanI G'GyrC_C

Cuts at:      0     791     853    1098 
   Size:        791      62     245

  Fragments arranged by size: 

                 791     245      62
 
BanII G_rGCy'C

Cuts at:      0     644     650    1098 
   Size:        644       6     448

  Fragments arranged by size: 

                 644     448       6
 
BbeI G_GCGC'C

Cuts at:      0     857    1098 
   Size:        857     241
 
BbvCI CC'TCA_GC

Cuts at:      0     206    1098 
   Size:        206     892
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     950     984    1098 
   Size:        950      34     114

  Fragments arranged by size: 

                 950     114      34
 
BglII A'GATC_T

Cuts at:      0     731    1098 
   Size:        731     367
 
Bme1580I G_kGCm'C

Cuts at:      0     650     874    1098 
   Size:        650     224     224

  Fragments arranged by size: 

                 650     224     224
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     388    1098 
   Size:        388     710
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     464     646     765     977     994    1098 
   Size:        320     144     182     119     212      17     104

  Fragments arranged by size: 

                 320     212     182     144     119     104      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     206    1098 
   Size:        206     892
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       4     124     607    1098 
   Size:          4     120     483     491

  Fragments arranged by size: 

                 491     483     120       4
 
BsaHI Gr'CG_yC

Cuts at:      0     287     854    1098 
   Size:        287     567     244

  Fragments arranged by size: 

                 567     287     244
 
BsaWI w'CCGG_w

Cuts at:      0     438    1098 
   Size:        438     660
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     992    1022    1098 
   Size:        992      30      76

  Fragments arranged by size: 

                 992      76      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     532     535    1098 
   Size:        532       3     563

  Fragments arranged by size: 

                 563     532       3
 
BseYI C'CCAG_C

Cuts at:      0     430     581     669     925    1098 
   Size:        430     151      88     256     173

  Fragments arranged by size: 

                 430     256     173     151      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1098 
   Size:        118     980
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     552    1098 
   Size:        113     439     546

  Fragments arranged by size: 

                 546     439     113
 
BsmI GAATG_Cn'

Cuts at:      0     434    1098 
   Size:        434     664
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     791    1098 
   Size:        791     307
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     552     644     650     874    1098 
   Size:        113     439      92       6     224     224

  Fragments arranged by size: 

                 439     224     224     113      92       6
 
BspEI T'CCGG_A

Cuts at:      0     438    1098 
   Size:        438     660
 
BsrDI GCAATG_nn'

Cuts at:      0     744    1098 
   Size:        744     354
 
BsrFI r'CCGG_y

Cuts at:      0     785    1098 
   Size:        785     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30    1098 
   Size:         30    1068
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     124     906     933    1098 
   Size:        124     782      27     165

  Fragments arranged by size: 

                 782     165     124      27
 
BstYI r'GATC_y

Cuts at:      0      26     303     394     731    1098 
   Size:         26     277      91     337     367

  Fragments arranged by size: 

                 367     337     277      91      26
 
BtgI C'CryG_G

Cuts at:      0      86     365    1098 
   Size:         86     279     733

  Fragments arranged by size: 

                 733     279      86
 
BtsI GCAGTG_nn'

Cuts at:      0     953    1098 
   Size:        953     145
 
EaeI y'GGCC_r

Cuts at:      0     806    1098 
   Size:        806     292
 
EarI CTCTTCn'nnn_

Cuts at:      0      73     509    1010    1090    1098 
   Size:         73     436     501      80       8

  Fragments arranged by size: 

                 501     436      80      73       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     320     464     646     765     977     994    1098 
   Size:         54     266     144     182     119     212      17     104

  Fragments arranged by size: 

                 266     212     182     144     119     104      54      17
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     130     673    1098 
   Size:        130     543     425

  Fragments arranged by size: 

                 543     425     130
 
FspI TGC'GCA

Cuts at:      0    1070    1098 
   Size:       1070      28
 
HaeII r_GCGC'y

Cuts at:      0     857    1098 
   Size:        857     241
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     122     154    1007    1039    1067    1098 
   Size:        122      32     853      32      28      31

  Fragments arranged by size: 

                 853     122      32      32      31      28
 
HincII GTy'rAC

Cuts at:      0     750    1098 
   Size:        750     348
 
HindIII A'AGCT_T

Cuts at:      0     656    1098 
   Size:        656     442
 
KasI G'GCGC_C

Cuts at:      0     853    1098 
   Size:        853     245
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     786    1098 
   Size:        786     312
 
MspA1I CmG'CkG

Cuts at:      0     958     975    1098 
   Size:        958      17     123

  Fragments arranged by size: 

                 958     123      17
 
NaeI GCC'GGC

Cuts at:      0     787    1098 
   Size:        787     311
 
NarI GG'CG_CC

Cuts at:      0     854    1098 
   Size:        854     244
 
NcoI C'CATG_G

Cuts at:      0      86     365    1098 
   Size:         86     279     733

  Fragments arranged by size: 

                 733     279      86
 
NgoMIV G'CCGG_C

Cuts at:      0     785    1098 
   Size:        785     313
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     334    1098 
   Size:        334     764
 
PpuMI rG'GwC_Cy

Cuts at:      0     130    1098 
   Size:        130     968
 
PspOMI G'GGCC_C

Cuts at:      0     646    1098 
   Size:        646     452
 
PspXI vC'TCGA_Gb

Cuts at:      0      43    1098 
   Size:         43    1055
 
PstI C_TGCA'G

Cuts at:      0     948    1098 
   Size:        948     150
 
SanDI GG'GwC_CC

Cuts at:      0     130    1098 
   Size:        130     968
 
SfcI C'TryA_G

Cuts at:      0      78     944    1098 
   Size:         78     866     154

  Fragments arranged by size: 

                 866     154      78
 
SfoI GGC'GCC

Cuts at:      0     855    1098 
   Size:        855     243
 
SmaI CCC'GGG

Cuts at:      0     686    1098 
   Size:        686     412
 
SmlI C'TyrA_G

Cuts at:      0      19      43     139     586    1098 
   Size:         19      24      96     447     512

  Fragments arranged by size: 

                 512     447      96      24      19
 
StyI C'CwwG_G

Cuts at:      0      60      86     365     461     887    1098 
   Size:         60      26     279      96     426     211

  Fragments arranged by size: 

                 426     279     211      96      60      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     728    1098 
   Size:        728     370
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     992    1024    1098 
   Size:        992      32      74

  Fragments arranged by size: 

                 992      74      32
 
XhoI C'TCGA_G

Cuts at:      0      43    1098 
   Size:         43    1055
 
XmaI C'CCGG_G

Cuts at:      0     684    1098 
   Size:        684     414
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI     ApaI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BsrDI    BsrFI   BstAPI    BstXI
    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FspI
    HaeII    Hin4I   HincII  HindIII     KasI     MmeI   MspA1I     NaeI
     NarI     NcoI   NgoMIV    PflMI    PpuMI   PspOMI    PspXI     PstI
    SanDI     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TstI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsiEI
    BsiWI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    FspAI     HpaI     KpnI     MfeI     MluI     MscI     MslI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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