Restriction map for pF1KE0784
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AfaI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BseRI       BsiWI       BsmI        Bsp1407I    
BspDI       BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       Bsu36I      
Cac8I       CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HpaI        HphI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MluNI       MscI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuII       RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          293  GTATAC               GT^[AC][GT]AC
AccIII        582  TCCGGA               T^CCGGA
AciI         1130  CCGC                 CCGC(-3/-1)
AflII         909  CTTAAG               C^TTAAG
AflIII       1211  ACATGT               A^C[GA][CT]GT
AspHI         236  GAGCAC               G[AT]GC[AT]^C
AvaI           77  CTCGAG               C^[CT]CG[GA]G
BanII         220  GAGCCC               G[GA]GC[CT]^C
BbvI         1220  GCTGC                GCAGC(8/12)
BfrI          909  CTTAAG               C^TTAAG
BsaJI         742  CCTTGG               C^C[ACGT][ACGT]GG
BsaWI         582  TCCGGA               [AT]^CCGG[AT]
BseAI         582  TCCGGA               T^CCGGA
BsgI         1004  GTGCAG               GTGCAG(16/14)
BsiEI          61  CGATCG               CG[GA][CT]^CG
BsiHKAI       236  GAGCAC               G[AT]GC[AT]^C
BsmBI         686  GAGACG               CGTCTC(1/5)
BsoBI          77  CTCGAG               C^[CT]CG[GA]G
BspEI         582  TCCGGA               T^CCGGA
BspHI         419  TCATGA               T^CATGA
BspLU11I     1211  ACATGT               A^CATGT
Bst1107I      293  GTATAC               GTA^TAC
BstZ17I       293  GTATAC               GTA^TAC
CfoI         1260  GCGC                 GCG^C
DraII         357  GGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI           50  GACCTAGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          683  GAAGAG               CTCTTC(1/4)
EcoNI         742  CCTTGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      357  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       742  CCTTGG               C^C[AT][AT]GG
Fnu4HI       1220  GCTGC                GC^[ACGT]GC
HaeII        1259  AGCGCC               [GA]GCGC^[CT]
HaeIII        316  GGCC                 GG^CC
HapII         583  CCGG                 C^CGG
HhaI         1260  GCGC                 GCG^C
HinP1I       1260  GCGC                 G^CGC
HinfI         263  GATTC                G^A[ACGT]TC
HpaII         583  CCGG                 C^CGG
ItaI         1220  GCTGC                GC^[ACGT]GC
Ksp632I       683  GAAGAG               CTCTTC(1/4)
MaeII        1137  ACGT                 A^CGT
MaeIII        389  GTGAC                ^GT[ACGT]AC
MroI          582  TCCGGA               T^CCGGA
MspI          583  CCGG                 C^CGG
MwoI          345  GCTGGAACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          709  CATATG               CA^TATG
NspI         1211  ACATGT               [GA]CATG^[CT]
PaeR7I         77  CTCGAG               C^TCGAG
PpuMI         357  GGGACCT              [GA]G^G[AT]CC[CT]
PvuI           61  CGATCG               CGAT^CG
RcaI          419  TCATGA               T^CATGA
SexAI        1082  ACCAGGT              A^CC[AT]GGT
SfaNI        1065  GATGC                GCATC(5/9)
StyI          742  CCTTGG               C^C[AT][AT]GG
TfiI          263  GATTC                G^A[AT]TC
TseI         1220  GCTGC                G^C[AT]GC
Tsp45I        389  GTGAC                ^GT[GC]AC
TspRI         998  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI           77  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          183  AAATTT               [GA]^AATT[CT]
              834  AAATTT              
ApoI          183  AAATTT               [GA]^AATT[CT]
              834  AAATTT              
Asp700I       395  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              832  GAAAATTTTC          
AvaII         118  GGACC                G^G[AT]CC
              358  GGACC               
BmyI          220  GAGCCC               G[AGT]GC[ACT]^C
              236  GAGCAC              
BsiYI         742  CCTTGGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1126  CCCACCGCAGG         
BslI          742  CCTTGGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1126  CCCACCGCAGG         
BsmAI         586  GAGAC                GTCTC(1/5)
              686  GAGAC               
BsmFI          66  GGGAC                GGGAC(10/14)
              357  GGGAC               
Bsp1286I      220  GAGCCC               G[AGT]GC[ACT]^C
              236  GAGCAC              
BssKI         517  CCAGG                ^CC[ACGT]GG
             1083  CCAGG               
BstF5I        148  GGATG                GGATG(2/0)
             1182  GGATG               
BstNI         517  CCAGG                CC^[AT]GG
             1083  CCAGG               
BstYI         280  GGATCT               [GA]^GATC[CT]
             1017  AGATCC              
DdeI          494  CTGAG                C^T[ACGT]AG
              727  CTGAG               
EcoRII        517  CCAGG                ^CC[AT]GG
             1083  CCAGG               
FokI          148  GGATG                GGATG(9/13)
             1182  GGATG               
HindIII       698  AAGCTT               A^AGCTT
              916  AAGCTT              
MflI          280  GGATCT               [GA]^GATC[CT]
             1017  AGATCC              
MvaI          517  CCAGG                CC^[AT]GG
             1083  CCAGG               
NlaIV         219  GGAGCC               GG[ACGT]^[ACGT]CC
              357  GGGACC              
ScrFI         517  CCAGG                CC^[ACGT]GG
             1083  CCAGG               
XhoII         280  GGATCT               [GA]^GATC[CT]
             1017  AGATCC              
XmnI          395  GAACATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              832  GAAAATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AlwI        BbsI        BfaI        BpmI        
BpuAI       BsrI        Cfr13I      DpnII       MaeI        
MboI        MboII       MnlI        MseI        NdeII       
NlaIII      Sau3AI      Sau96I      SmlI        TaqI        
Tru9I       Tsp509I     TthHB8I     
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