Restriction map for
pF1KA1662
(Circular) (Six-base) MAPSORT of: pF1KA1662.seq Check: 4931 from: 1 to: 10208
pF1KA1662 10208 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 288 3297 4158 6468 6705 7151 288
Size: 3009 861 2310 237 446 3345
Fragments arranged by size:
3345 3009 2310 861 446 237
AatII G_ACGT'C
Cuts at: 7079 7079
Size: 10208
AccI GT'mk_AC
Cuts at: 7246 7246
Size: 10208
Acc65I G'GTAC_C
Cuts at: 3452 7224 3452
Size: 3772 6436
AclI AA'CG_TT
Cuts at: 10048 10048
Size: 10208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 3409 5759 5875 6721 7153 8033 8465 8818
Size: 3188 2350 116 846 432 880 432 353
Cuts at: 8818 9480 221
Size: 662 949
Fragments arranged by size:
3188 2350 949 880 846 662 432 432
353 116
AfeI AGC'GCT
Cuts at: 6052 7051 6052
Size: 999 9209
AflIII A'CryG_T
Cuts at: 922 7560 9360 9641 922
Size: 6638 1800 281 1489
Fragments arranged by size:
6638 1800 1489 281
AgeI A'CCGG_T
Cuts at: 815 815
Size: 10208
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 10208
AleI CACnn'nnGTG
Cuts at: 3441 3595 4714 3441
Size: 154 1119 8935
Fragments arranged by size:
8935 1119 154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3188 3632 3788 3188
Size: 444 156 9608
Fragments arranged by size:
9608 444 156
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1347 1379 1494 1526 1641 1673 1788 1820 1935
Size: 32 115 32 115 32 115 32 115
Cuts at: 1935 1967 1347
Size: 32 9588
Fragments arranged by size:
9588 115 115 115 115 32 32 32
32 32
AlwNI CAG_nnn'CTG
Cuts at: 244 4377 6204 6525 6825 6846 7513 8951 244
Size: 4133 1827 321 300 21 667 1438 1501
Fragments arranged by size:
4133 1827 1501 1438 667 321 300 21
ApaI G_GGCC'C
Cuts at: 356 367 3974 4423 4474 4833 5164 5194 5851
Size: 11 3607 449 51 359 331 30 657
Cuts at: 5851 5985 6287 6339 6658 356
Size: 134 302 52 319 3906
Fragments arranged by size:
3906 3607 657 449 359 331 319 302
134 52 51 30 11
ApaLI G'TGCA_C
Cuts at: 6380 9046 6380
Size: 2666 7542
ApoI r'AATT_y
Cuts at: 1308 2787 7212 9561 9817 1308
Size: 1479 4425 2349 256 1699
Fragments arranged by size:
4425 2349 1699 1479 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 10208
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10208
AvaI C'yCGr_G
Cuts at: 630 641 3029 3179 3320 3467 3779 3863 3880
Size: 11 2388 150 141 147 312 84 17
Cuts at: 3880 4771 5365 6414 7059 7228 630
Size: 891 594 1049 645 169 3610
Fragments arranged by size:
3610 2388 1049 891 645 594 312 169
150 147 141 84 17 11
AvrII C'CTAG_G
Cuts at: 1066 1066
Size: 10208
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3076 3109 6973 7006 3076
Size: 33 3864 33 6278
Fragments arranged by size:
6278 3864 33 33
BamHI G'GATC_C
Cuts at: 3675 7233 3675
Size: 3558 6650
BanI G'GyrC_C
Cuts at: 117 249 812 3164 3452 3608 3764 3828 4809
Size: 132 563 2352 288 156 156 64 981
Cuts at: 4809 6105 6139 6237 6500 7224 7638 7886 7921
Size: 1296 34 98 263 724 414 248 35
Cuts at: 7921 117
Size: 2404
Fragments arranged by size:
2404 2352 1296 981 724 563 414 288
263 248 156 156 132 98 64 35
34
BanII G_rGCy'C
Cuts at: 227 235 356 367 386 617 678 3114 3134
Size: 8 121 11 19 231 61 2436 20
Cuts at: 3134 3239 3422 3862 3871 3974 4423 4474 4502
Size: 105 183 440 9 103 449 51 28
Cuts at: 4502 4613 4651 4833 4871 5059 5164 5194 5329
Size: 111 38 182 38 188 105 30 135
Cuts at: 5329 5851 5881 5951 5985 6287 6339 6658 7028
Size: 522 30 70 34 302 52 319 370
Cuts at: 7028 7222 8252 227
Size: 194 1030 2183
Fragments arranged by size:
2436 2183 1030 522 449 440 370 319
302 231 194 188 183 182 135 121
111 105 105 103 70 61 52 51
38 38 34 30 30 28 20 19
11 9 8
BbeI G_GCGC'C
Cuts at: 7642 7890 7642
Size: 248 9960
BbsI GAAGACnn'nnnn_
Cuts at: 4146 4146
Size: 10208
BbvCI CC'TCA_GC
Cuts at: 245 375 5588 6554 6576 6776 7199 245
Size: 130 5213 966 22 200 423 3254
Fragments arranged by size:
5213 3254 966 423 200 130 22
BciVI GTATCCnnnnn_n'
Cuts at: 8106 9162 10208 8106
Size: 1056 1046 8106
Fragments arranged by size:
8106 1056 1046
BclI T'GATC_A
Cuts at: 7728 7728
Size: 10208
BglI GCCn_nnn'nGGC
Cuts at: 3109 3322 4081 4192 4361 4478 5900 6662 3109
Size: 213 759 111 169 117 1422 762 6655
Fragments arranged by size:
6655 1422 762 759 213 169 117 111
BglII A'GATC_T
Cuts at: 7723 9760 7723
Size: 2037 8171
BlpI GC'TnA_GC
Cuts at: 3870 6616 6628 7090 7320 3870
Size: 2746 12 462 230 6758
Fragments arranged by size:
6758 2746 462 230 12
Bme1580I G_kGCm'C
Cuts at: 254 356 367 1329 1476 1554 1623 1770 1848
Size: 102 11 962 147 78 69 147 78
Cuts at: 1848 1917 1995 2067 2139 2211 2283 2355 2496
Size: 69 78 72 72 72 72 72 141
Cuts at: 2496 3167 3580 3611 3767 3974 4423 4474 4546
Size: 671 413 31 156 207 449 51 72
Cuts at: 4546 4812 4833 5164 5194 5851 5985 6144 6242
Size: 266 21 331 30 657 134 159 98
Cuts at: 6242 6287 6339 6384 6658 6762 7833 7926 9050
Size: 45 52 45 274 104 1071 93 1124
Cuts at: 9050 254
Size: 1412
Fragments arranged by size:
1412 1124 1071 962 671 657 449 413
331 274 266 207 159 156 147 147
141 134 104 102 98 93 78 78
78 72 72 72 72 72 72 69
69 52 51 45 45 31 30 21
11
BmgBI CAC'GTC
Cuts at: 875 875
Size: 10208
BmrI ACTGGGnnnn_n'
Cuts at: 576 780 2985 3006 3730 3803 4671 4860 5014
Size: 204 2205 21 724 73 868 189 154
Cuts at: 5014 5088 7420 7501 7836 9980 576
Size: 74 2332 81 335 2144 804
Fragments arranged by size:
2332 2205 2144 868 804 724 335 204
189 154 81 74 73 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 656 2653 5508 6750 8575 656
Size: 1997 2855 1242 1825 2289
Fragments arranged by size:
2855 2289 1997 1825 1242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 498 826 960 990 1020 1335 1482 1629
Size: 26 328 134 30 30 315 147 147
Cuts at: 1629 1776 1923 4258 4366 4467 4803 5170 5501
Size: 147 147 2335 108 101 336 367 331
Cuts at: 5501 6052 6226 6331 6364 6631 6769 6970 7012
Size: 551 174 105 33 267 138 201 42
Cuts at: 7012 7150 472
Size: 138 3530
Fragments arranged by size:
3530 2335 551 367 336 331 328 315
267 201 174 147 147 147 147 138
138 134 108 105 101 42 33 30
30 26
Bpu10I CC'TnA_GC
Cuts at: 226 245 375 908 3128 3653 4973 5588 6554
Size: 19 130 533 2220 525 1320 615 966
Cuts at: 6554 6576 6776 7199 226
Size: 22 200 423 3235
Fragments arranged by size:
3235 2220 1320 966 615 533 525 423
200 130 22 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 5317 7384 8263 8977 9275 454
Size: 4863 2067 879 714 298 1387
Fragments arranged by size:
4863 2067 1387 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2736 3880 4245 4332 9912 2736
Size: 1144 365 87 5580 3032
Fragments arranged by size:
5580 3032 1144 365 87
BsaAI yAC'GTr
Cuts at: 925 5943 7561 925
Size: 5018 1618 3572
Fragments arranged by size:
5018 3572 1618
BsaBI GATnn'nnATC
Cuts at: 6 5464 7722 9759 6
Size: 5458 2258 2037 455
Fragments arranged by size:
5458 2258 2037 455
BsaHI Gr'CG_yC
Cuts at: 3325 6354 7076 7639 7887 8589 10085 3325
Size: 3029 722 563 248 702 1496 3448
Fragments arranged by size:
3448 3029 1496 722 702 563 248
BsaWI w'CCGG_w
Cuts at: 815 6668 7397 7918 8596 9007 9154 9428 815
Size: 5853 729 521 678 411 147 274 1595
Fragments arranged by size:
5853 1595 729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 1206 1236 1347 1377 1494 1524 1641
Size: 30 680 30 111 30 117 30 117
Cuts at: 1641 1671 1788 1818 1935 1965 2436 2466 2943
Size: 30 117 30 117 30 471 30 477
Cuts at: 2943 2973 4808 4838 5563 5593 6526 6556 6629
Size: 30 1835 30 725 30 933 30 73
Cuts at: 6629 6659 6994 7024 7162 7192 496
Size: 30 335 30 138 30 3512
Fragments arranged by size:
3512 1835 933 725 680 477 471 335
138 117 117 117 117 111 73 30
30 30 30 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 211 604 607 648 676 722 933 1269
Size: 84 393 3 41 28 46 211 336
Cuts at: 1269 1350 1419 1497 1566 1644 1713 1791 1860
Size: 81 69 78 69 78 69 78 69
Cuts at: 1860 1938 2007 2079 2151 2223 2295 2367 2439
Size: 78 69 72 72 72 72 72 72
Cuts at: 2439 2508 2697 3465 3777 3925 4606 5239 5248
Size: 69 189 768 312 148 681 633 9
Cuts at: 5248 5605 6040 6073 6193 6362 6502 6571 6793
Size: 357 435 33 120 169 140 69 222
Cuts at: 6793 6880 127
Size: 87 3455
Fragments arranged by size:
3455 768 681 633 435 393 357 336
312 222 211 189 169 148 140 120
87 84 81 78 78 78 78 72
72 72 72 72 72 69 69 69
69 69 69 69 46 41 33 28
9 3
BseYI C'CCAG_C
Cuts at: 601 605 1184 1226 1328 1406 1475 1553 1622
Size: 4 579 42 102 78 69 78 69
Cuts at: 1622 1700 1769 1847 1916 1994 2167 2354 2495
Size: 78 69 78 69 78 173 187 141
Cuts at: 2495 2962 2975 3137 4111 4159 4501 4568 4721
Size: 467 13 162 974 48 342 67 153
Cuts at: 4721 4840 4899 4944 5003 5071 5194 5320 5991
Size: 119 59 45 59 68 123 126 671
Cuts at: 5991 6047 6338 7633 8391 9056 9464 601
Size: 56 291 1295 758 665 408 1345
Fragments arranged by size:
1345 1295 974 758 671 665 579 467
408 342 291 187 173 162 153 141
126 123 119 102 78 78 78 78
78 69 69 69 69 68 67 59
59 56 48 45 42 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1430 1724 2706 3419 3553 4005 4040 5067 5616
Size: 294 982 713 134 452 35 1027 549
Cuts at: 5616 5677 5809 7027 1430
Size: 61 132 1218 4611
Fragments arranged by size:
4611 1218 1027 982 713 549 452 294
134 132 61 35
BsiEI CG_ry'CG
Cuts at: 104 5132 7750 7796 9026 104
Size: 5028 2618 46 1230 1286
Fragments arranged by size:
5028 2618 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 227 617 3070 4613 4871 5182 5951 6384 6791
Size: 390 2453 1543 258 311 769 433 407
Cuts at: 6791 7028 7142 7222 8000 8190 9050 227
Size: 237 114 80 778 190 860 1385
Fragments arranged by size:
2453 1543 1385 860 778 769 433 407
390 311 258 237 190 114 80
BsiWI C'GTAC_G
Cuts at: 5763 5763
Size: 10208
BsmBI CGTCTCn'nnnn_
Cuts at: 686 881 6361 6553 686
Size: 195 5480 192 4341
Fragments arranged by size:
5480 4341 195 192
Bsp1286I G_dGCh'C
Cuts at: 227 235 254 356 367 386 617 678 1329
Size: 8 19 102 11 19 231 61 651
Cuts at: 1329 1476 1554 1623 1770 1848 1917 1995 2067
Size: 147 78 69 147 78 69 78 72
Cuts at: 2067 2139 2211 2283 2355 2496 3070 3114 3134
Size: 72 72 72 72 141 574 44 20
Cuts at: 3134 3167 3239 3422 3580 3611 3767 3862 3871
Size: 33 72 183 158 31 156 95 9
Cuts at: 3871 3974 4423 4474 4502 4546 4613 4651 4812
Size: 103 449 51 28 44 67 38 161
Cuts at: 4812 4833 4871 5059 5164 5182 5194 5329 5851
Size: 21 38 188 105 18 12 135 522
Cuts at: 5851 5881 5951 5985 6144 6242 6287 6339 6384
Size: 30 70 34 159 98 45 52 45
Cuts at: 6384 6658 6762 6791 7028 7142 7222 7833 7926
Size: 274 104 29 237 114 80 611 93
Cuts at: 7926 8000 8190 8252 9050 227
Size: 74 190 62 798 1385
Fragments arranged by size:
1385 798 651 611 574 522 449 274
237 231 190 188 183 161 159 158
156 147 147 141 135 114 105 104
103 102 98 95 93 80 78 78
78 74 72 72 72 72 72 72
70 69 69 67 62 61 52 51
45 45 44 44 38 38 34 33
31 30 29 28 21 20 19 19
18 12 11 9 8
BspEI T'CCGG_A
Cuts at: 6668 6668
Size: 10208
BspHI T'CATG_A
Cuts at: 4605 8640 4605
Size: 4035 6173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 272 288 3297 3941 4158 5630 6468 6705 7151
Size: 16 3009 644 217 1472 838 237 446
Cuts at: 7151 7258 7472 7774 8155 272
Size: 107 214 302 381 2325
Fragments arranged by size:
3009 2325 1472 838 644 446 381 302
237 217 214 107 16
BsrBI CCG'CTC
Cuts at: 50 810 2762 3906 5331 6036 6063 6510 6936
Size: 760 1952 1144 1425 705 27 447 426
Cuts at: 6936 7116 8500 8554 10038 10203 50
Size: 180 1384 54 1484 165 55
Fragments arranged by size:
1952 1484 1425 1384 1144 760 705 447
426 180 165 55 54 27
BsrDI GCAATG_nn'
Cuts at: 6890 6918 8120 6890
Size: 28 1202 8978
Fragments arranged by size:
8978 1202 28
BsrFI r'CCGG_y
Cuts at: 739 815 5372 6167 8206 8387 739
Size: 76 4557 795 2039 181 2560
Fragments arranged by size:
4557 2560 2039 795 181 76
BsrGI T'GTAC_A
Cuts at: 2650 2650
Size: 10208
BssSI C'ACGA_G
Cuts at: 1379 1448 1526 1595 1673 1742 1820 1889 1967
Size: 69 78 69 78 69 78 69 78
Cuts at: 1967 2468 3268 3415 3571 4644 6301 6505 8479
Size: 501 800 147 156 1073 1657 204 1974
Cuts at: 8479 9187 1379
Size: 708 2400
Fragments arranged by size:
2400 1974 1657 1073 800 708 501 204
156 147 78 78 78 78 69 69
69 69
BstAPI GCAn_nnn'nTGC
Cuts at: 6525 6795 6917 7585 6525
Size: 270 122 668 9148
Fragments arranged by size:
9148 668 270 122
BstBI TT'CG_AA
Cuts at: 8569 8569
Size: 10208
BstEII G'GTnAC_C
Cuts at: 4170 5346 4170
Size: 1176 9032
BstXI CCAn_nnnn'nTGG
Cuts at: 3951 4586 4716 5003 6400 3951
Size: 635 130 287 1397 7759
Fragments arranged by size:
7759 1397 635 287 130
BstYI r'GATC_y
Cuts at: 1 3675 4221 4788 5678 6388 7233 7700 7723
Size: 3674 546 567 890 710 845 467 23
Cuts at: 7723 8058 8304 8610 8622 8708 9760 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
3674 1052 890 845 710 567 546 467
449 335 306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 194 287 343 4256 5231 5416 5721 194
Size: 93 56 3913 975 185 305 4681
Fragments arranged by size:
4681 3913 975 305 185 93 56
BtgI C'CryG_G
Cuts at: 107 204 3557 6267 6569 7154 107
Size: 97 3353 2710 302 585 3161
Fragments arranged by size:
3353 3161 2710 585 302 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2901 8158 8339 8502 2901
Size: 5257 181 163 4607
Fragments arranged by size:
5257 4607 181 163
BtsI GCAGTG_nn'
Cuts at: 626 902 3988 4709 5041 5180 5530 5582 6245
Size: 276 3086 721 332 139 350 52 663
Cuts at: 6245 7015 7451 626
Size: 770 436 3383
Fragments arranged by size:
3383 3086 770 721 663 436 350 332
276 139 52
DraI TTT'AAA
Cuts at: 7207 7207
Size: 10208
DraIII CAC_nnn'GTG
Cuts at: 856 1252 3811 5096 856
Size: 396 2559 1285 5968
Fragments arranged by size:
5968 2559 1285 396
DrdI GACnn_nn'nnGTC
Cuts at: 7914 9258 7914
Size: 1344 8864
EaeI y'GGCC_r
Cuts at: 2842 2926 3926 4014 4353 4922 5098 5711 6014
Size: 84 1000 88 339 569 176 613 303
Cuts at: 6014 6297 6402 6852 7157 7793 7967 8358 8385
Size: 283 105 450 305 636 174 391 27
Cuts at: 8385 9730 2842
Size: 1345 3320
Fragments arranged by size:
3320 1345 1000 636 613 569 450 391
339 305 303 283 176 174 105 88
84 27
EagI C'GGCC_G
Cuts at: 7793 7793
Size: 10208
EarI CTCTTCn'nnn_
Cuts at: 386 1182 2877 3045 3192 3670 4042 4243 6335
Size: 796 1695 168 147 478 372 201 2092
Cuts at: 6335 6605 8231 8441 386
Size: 270 1626 210 2153
Fragments arranged by size:
2153 2092 1695 1626 796 478 372 270
210 201 168 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 516 4224 5373 5748 5929 8416 9158 9304 10018
Size: 3708 1149 375 181 2487 742 146 714
Cuts at: 10018 516
Size: 706
Fragments arranged by size:
3708 2487 1149 742 714 706 375 181
146
EcoICRI GAG'CTC
Cuts at: 225 615 4611 4869 5949 7026 7220 225
Size: 390 3996 258 1080 1077 194 3213
Fragments arranged by size:
3996 3213 1080 1077 390 258 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 221 472 498 826 960 990 1020 1335 1482
Size: 251 26 328 134 30 30 315 147
Cuts at: 1482 1629 1776 1923 3409 4258 4366 4467 4803
Size: 147 147 147 1486 849 108 101 336
Cuts at: 4803 5170 5501 5759 5875 6052 6226 6331 6364
Size: 367 331 258 116 177 174 105 33
Cuts at: 6364 6631 6721 6769 6970 7012 7150 7153 8033
Size: 267 90 48 201 42 138 3 880
Cuts at: 8033 8465 8818 9480 221
Size: 432 353 662 949
Fragments arranged by size:
1486 949 880 849 662 432 367 353
336 331 328 315 267 258 251 201
177 174 147 147 147 147 138 134
116 108 105 101 90 48 42 33
30 30 26 3
EcoNI CCTnn'n_nnAGG
Cuts at: 704 6151 6529 6553 6862 6997 7198 704
Size: 5447 378 24 309 135 201 3714
Fragments arranged by size:
5447 3714 378 309 201 135 24
EcoO109I rG'GnC_Cy
Cuts at: 291 352 363 397 456 492 597 695 980
Size: 61 11 34 59 36 105 98 285
Cuts at: 980 1010 1040 1070 1100 2444 3492 3519 3970
Size: 30 30 30 30 1344 1048 27 451
Cuts at: 3970 3971 4073 4280 4310 4412 4419 4420 4471
Size: 1 102 207 30 102 7 1 51
Cuts at: 4471 4669 4857 4896 4948 5161 5190 5191 5412
Size: 198 188 39 52 213 29 1 221
Cuts at: 5412 5847 5848 6039 6095 6132 6283 6284 6654
Size: 435 1 191 56 37 151 1 370
Cuts at: 6654 7347 291
Size: 693 3152
Fragments arranged by size:
3152 1344 1048 693 451 435 370 285
221 213 207 198 191 188 151 105
102 102 98 61 59 56 52 51
39 37 36 34 30 30 30 30
30 29 27 11 7 1 1 1
1 1
EcoRI G'AATT_C
Cuts at: 1308 2787 7212 1308
Size: 1479 4425 4304
Fragments arranged by size:
4425 4304 1479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8227 8227
Size: 10208
FspI TGC'GCA
Cuts at: 7186 7989 7186
Size: 803 9405
HaeII r_GCGC'y
Cuts at: 305 3877 4035 6054 6279 6525 7053 7642 7890
Size: 3572 158 2019 225 246 528 589 248
Cuts at: 7890 9120 9891 305
Size: 1230 771 622
Fragments arranged by size:
3572 2019 1230 771 622 589 528 248
246 225 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 484 496 516 528 2436 2468 2844 2876 2943
Size: 12 20 12 1908 32 376 32 67
Cuts at: 2943 2975 3607 3639 4084 4116 4808 4840 4966
Size: 32 632 32 445 32 692 32 126
Cuts at: 4966 4998 5121 5153 5733 5751 5765 5783 6448
Size: 32 123 32 580 18 14 18 665
Cuts at: 6448 6480 7162 7194 8050 8080 8082 8112 484
Size: 32 682 32 856 30 2 30 2580
Fragments arranged by size:
2580 1908 856 692 682 665 632 580
445 376 126 123 67 32 32 32
32 32 32 32 32 32 32 30
30 20 18 18 14 12 12 2
HincII GTy'rAC
Cuts at: 821 7247 821
Size: 6426 3782
KasI G'GCGC_C
Cuts at: 7638 7886 7638
Size: 248 9960
KpnI G_GTAC'C
Cuts at: 3456 7228 3456
Size: 3772 6436
MluI A'CGCG_T
Cuts at: 9641 9641
Size: 10208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2133 2205 2421 5241 5794 8967 9151 2133
Size: 72 216 2820 553 3173 184 3190
Fragments arranged by size:
3190 3173 2820 553 216 184 72
MscI TGG'CCA
Cuts at: 4355 4924 5100 6016 6404 7969 4355
Size: 569 176 916 388 1565 6594
Fragments arranged by size:
6594 1565 916 569 388 176
MslI CAynn'nnrTG
Cuts at: 2715 3170 3311 3441 3595 3614 3770 4714 4913
Size: 455 141 130 154 19 156 944 199
Cuts at: 4913 8197 8324 2715
Size: 3284 127 4599
Fragments arranged by size:
4599 3284 944 455 199 156 154 141
130 127 19
MspA1I CmG'CkG
Cuts at: 173 406 482 605 1230 1332 1410 1479 1557
Size: 233 76 123 625 102 78 69 78
Cuts at: 1557 1626 1704 1773 1851 1920 1998 2358 2499
Size: 69 78 69 78 69 78 360 141
Cuts at: 2499 2658 3100 3992 4069 4287 4903 5067 5871
Size: 159 442 892 77 218 616 164 804
Cuts at: 5871 5958 5979 6269 6342 6498 6803 6879 7131
Size: 87 21 290 73 156 305 76 252
Cuts at: 7131 7319 7633 7993 8777 9022 173
Size: 188 314 360 784 245 1359
Fragments arranged by size:
1359 892 804 784 625 616 442 360
360 314 305 290 252 245 233 218
188 164 159 156 141 123 102 87
78 78 78 78 78 77 76 76
73 69 69 69 69 21
NaeI GCC'GGC
Cuts at: 8389 8389
Size: 10208
NarI GG'CG_CC
Cuts at: 7639 7887 7639
Size: 248 9960
NcoI C'CATG_G
Cuts at: 107 204 3557 7154 107
Size: 97 3353 3597 3161
Fragments arranged by size:
3597 3353 3161 97
NgoMIV G'CCGG_C
Cuts at: 8387 8387
Size: 10208
NspI r_CATG'y
Cuts at: 6440 7261 7761 9364 6440
Size: 821 500 1603 7284
Fragments arranged by size:
7284 1603 821 500
PasI CC'CwG_GG
Cuts at: 1097 2822 2906 3527 4416 4474 5166 1097
Size: 1725 84 621 889 58 692 6139
Fragments arranged by size:
6139 1725 889 692 621 84 58
PciI A'CATG_T
Cuts at: 9360 9360
Size: 10208
PflMI CCAn_nnn'nTGG
Cuts at: 327 856 2771 5026 5096 327
Size: 529 1915 2255 70 5439
Fragments arranged by size:
5439 2255 1915 529 70
PfoI T'CCnGG_A
Cuts at: 3515 3671 7016 7166 3515
Size: 156 3345 150 6557
Fragments arranged by size:
6557 3345 156 150
PmeI GTTT'AAAC
Cuts at: 7207 7207
Size: 10208
PmlI CAC'GTG
Cuts at: 925 925
Size: 10208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1347 1379 1494 1526 1641 1673 1788 1820 1935
Size: 32 115 32 115 32 115 32 115
Cuts at: 1935 1967 5197 5229 8654 8686 1347
Size: 32 3230 32 3425 32 2869
Fragments arranged by size:
3425 3230 2869 115 115 115 115 32
32 32 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 291 397 492 695 2444 3519 4280 4310 4412
Size: 106 95 203 1749 1075 761 30 102
Cuts at: 4412 4896 4948 5412 6039 6095 291
Size: 484 52 464 627 56 4404
Fragments arranged by size:
4404 1749 1075 761 627 484 464 203
106 102 95 56 52 30
PspOMI G'GGCC_C
Cuts at: 352 363 3970 4419 4470 4829 5160 5190 5847
Size: 11 3607 449 51 359 331 30 657
Cuts at: 5847 5981 6283 6335 6654 352
Size: 134 302 52 319 3906
Fragments arranged by size:
3906 3607 657 449 359 331 319 302
134 52 51 30 11
PspXI vC'TCGA_Gb
Cuts at: 3179 3179
Size: 10208
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1164 1196 2406 2438 1164
Size: 32 1210 32 8934
Fragments arranged by size:
8934 1210 32 32
PstI C_TGCA'G
Cuts at: 720 4877 5048 5213 6497 6521 6530 6716 7255
Size: 4157 171 165 1284 24 9 186 539
Cuts at: 7255 7940 720
Size: 685 2988
Fragments arranged by size:
4157 2988 1284 685 539 186 171 165
24 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10208
PvuII CAG'CTG
Cuts at: 406 482 605 4903 5067 6342 6879 7131 7633
Size: 76 123 4298 164 1275 537 252 502
Cuts at: 7633 7993 406
Size: 360 2621
Fragments arranged by size:
4298 2621 1275 537 502 360 252 164
123 76
RsrII CG'GwC_CG
Cuts at: 8403 8403
Size: 10208
SacI G_AGCT'C
Cuts at: 227 617 4613 4871 5951 7028 7222 227
Size: 390 3996 258 1080 1077 194 3213
Fragments arranged by size:
3996 3213 1080 1077 390 258 194
SacII CC_GC'GG
Cuts at: 6270 6270
Size: 10208
SalI G'TCGA_C
Cuts at: 7245 7245
Size: 10208
SanDI GG'GwC_CC
Cuts at: 397 4280 4412 4948 397
Size: 3883 132 536 5657
Fragments arranged by size:
5657 3883 536 132
SapI GCTCTTCn'nnn_
Cuts at: 6335 6605 8231 8441 6335
Size: 270 1626 210 8102
Fragments arranged by size:
8102 1626 270 210
SbfI CC_TGCA'GG
Cuts at: 4877 7255 4877
Size: 2378 7830
ScaI AGT'ACT
Cuts at: 6733 9725 6733
Size: 2992 7216
SexAI A'CCwGG_T
Cuts at: 691 691
Size: 10208
SfcI C'TryA_G
Cuts at: 33 716 4873 5044 5209 5832 6493 6517 6526
Size: 683 4157 171 165 623 661 24 9
Cuts at: 6526 6712 7251 7936 8904 9095 33
Size: 186 539 685 968 191 1146
Fragments arranged by size:
4157 1146 968 685 683 661 623 539
191 186 171 165 24 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 4081 4361 6662 4081
Size: 280 2301 7627
Fragments arranged by size:
7627 2301 280
SfoI GGC'GCC
Cuts at: 7640 7888 7640
Size: 248 9960
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10208
SgrAI Cr'CCGG_yG
Cuts at: 739 739
Size: 10208
SmaI CCC'GGG
Cuts at: 3031 3322 3469 3865 3882 5367 6416 7230 3031
Size: 291 147 396 17 1485 1049 814 6009
Fragments arranged by size:
6009 1485 1049 814 396 291 147 17
SmlI C'TyrA_G
Cuts at: 433 3179 5332 7363 8278 8992 9254 433
Size: 2746 2153 2031 915 714 262 1387
Fragments arranged by size:
2746 2153 2031 1387 915 714 262
SpeI A'CTAG_T
Cuts at: 67 3688 67
Size: 3621 6587
SphI G_CATG'C
Cuts at: 7261 7761 7261
Size: 500 9708
SspI AAT'ATT
Cuts at: 5569 5569
Size: 10208
StuI AGG'CCT
Cuts at: 341 639 2946 4466 4585 5147 5932 6601 341
Size: 298 2307 1520 119 562 785 669 3948
Fragments arranged by size:
3948 2307 1520 785 669 562 298 119
StyI C'CwwG_G
Cuts at: 107 204 1066 2934 3557 4116 5687 5933 6360
Size: 97 862 1868 623 559 1571 246 427
Cuts at: 6360 7154 7342 7696 107
Size: 794 188 354 2619
Fragments arranged by size:
2619 1868 1571 862 794 623 559 427
354 246 188 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 976 1006 1036 1066 1096 1168 2050 2266 2551
Size: 30 30 30 30 72 882 216 285
Cuts at: 2551 2740 3025 3361 3818 3822 3957 4722 5107
Size: 189 285 336 457 4 135 765 385
Cuts at: 5107 5854 6127 7791 7924 8383 8592 976
Size: 747 273 1664 133 459 209 2592
Fragments arranged by size:
2592 1664 882 765 747 459 457 385
336 285 285 273 216 209 189 135
133 72 30 30 30 30 4
TatI w'GTAC_w
Cuts at: 1219 2650 3014 3914 4556 6731 9723 1219
Size: 1431 364 900 642 2175 2992 1704
Fragments arranged by size:
2992 2175 1704 1431 900 642 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 118 150 914 946 1236 1238 1268 1270 6526
Size: 32 764 32 290 2 30 2 5256
Cuts at: 6526 6558 6629 6661 6994 7026 9725 9757 118
Size: 32 71 32 333 32 2699 32 569
Fragments arranged by size:
5256 2699 764 569 333 290 71 32
32 32 32 32 32 30 2 2
Tth111I GACn'n_nGTC
Cuts at: 7107 8005 7107
Size: 898 9310
XbaI T'CTAG_A
Cuts at: 7239 7239
Size: 10208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 699 2998 3679 3897 6847 7703 699
Size: 2299 681 218 2950 856 3204
Fragments arranged by size:
3204 2950 2299 856 681 218
XhoI C'TCGA_G
Cuts at: 3179 3179
Size: 10208
XmaI C'CCGG_G
Cuts at: 3029 3320 3467 3863 3880 5365 6414 7228 3029
Size: 291 147 396 17 1485 1049 814 6009
Fragments arranged by size:
6009 1485 1049 814 396 291 147 17
XmnI GAAnn'nnTTC
Cuts at: 1176 2871 1176
Size: 1695 8513
ZraI GAC'GTC
Cuts at: 7077 7077
Size: 10208
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AflII AscI BcgI BfrBI BmtI BsmI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PshAI PsiI
SnaBI SrfI SwaI