Restriction map for pF1KE0636
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbsI        BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRV       FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MboII       MfeI        MflI        
MluI        MluNI       MroI        MscI        MspI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI          491  GCGG                 CCGC(-3/-1)
AfaI         1114  GTAC                 GT^AC
AflIII        780  ACACGT               A^C[GA][CT]GT
AlwI          168  GATCC                GGATC(4/5)
AlwNI         236  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII         693  GGTCC                G^G[AT]CC
BanII         228  GAGCTC               G[GA]GC[CT]^C
BbrPI         781  CACGTG               CAC^GTG
BclI            2  TGATCA               T^GATCA
BsaAI         781  CACGTG               [CT]AC^GT[GA]
BsaI          636  GAGACC               GGTCTC(1/5)
BsgI          732  GTGCAG               GTGCAG(16/14)
BsmFI        1017  GTCCC                GGGAC(10/14)
Bsp1407I     1113  TGTACA               T^GTACA
BsrGI        1113  TGTACA               T^GTACA
Cfr13I        693  GGTCC                G^G[ACGT]CC
DsaI          196  CCATGG               C^C[GA][CT]GG
EcoNI         584  CCTATAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         956  GAATTC               G^AATTC
EcoT14I       196  CCATGG               C^C[AT][AT]GG
EcoT22I       673  ATGCAT               ATGCA^T
FauI          491  GCGGG                CCCGC(4/6)
FbaI            2  TGATCA               T^GATCA
HindIII       917  AAGCTT               A^AGCTT
MaeII         782  ACGT                 A^CGT
MspA1I         67  CAGCTG               C[AC]G^C[GT]G
NcoI          196  CCATGG               C^CATGG
NdeI          906  CATATG               CA^TATG
NsiI          673  ATGCAT               ATGCA^T
PmaCI         781  CACGTG               CAC^GTG
PmlI          781  CACGTG               CAC^GTG
PstI           53  CTGCAG               CTGCA^G
PvuII          67  CAGCTG               CAG^CTG
RsaI         1114  GTAC                 GT^AC
SacI          228  GAGCTC               GAGCT^C
Sau96I        693  GGTCC                G^G[ACGT]CC
SfaNI         273  GCATC                GCATC(5/9)
SspBI        1113  TGTACA               T^GTACA
StyI          196  CCATGG               C^C[AT][AT]GG
Tsp45I         22  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AspHI         228  GAGCTC               G[AT]GC[AT]^C
              363  GAGCAC              
BbvI           66  GCAGC                GCAGC(8/12)
               69  GCTGC               
BmyI          228  GAGCTC               G[AGT]GC[ACT]^C
              363  GAGCAC              
BpmI          233  CTCCAG               CTGGAG(16/14)
              713  CTGGAG              
BsaJI         196  CCATGG               C^C[ACGT][ACGT]GG
              265  CCAGGG              
BsiHKAI       228  GAGCTC               G[AT]GC[AT]^C
              363  GAGCAC              
BsmAI         636  GAGAC                GTCTC(1/5)
              702  GAGAC               
Bsp1286I      228  GAGCTC               G[AGT]GC[ACT]^C
              363  GAGCAC              
BsrDI         566  GCAATG               GCAATG(2/0)
              650  GCAATG              
BsrI          712  ACTGG                ACTGG(1/-1)
              897  CCAGT               
BssSI         223  CACGAG               CACGAG(-5/-1)
              688  CTCGTG              
BstAPI        482  GCAGTGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              598  GCACACCTTGC         
DpnII           3  GATC                 ^GATC
              168  GATC                
Fnu4HI         66  GCAGC                GC^[ACGT]GC
               69  GCTGC               
ItaI           66  GCAGC                GC^[ACGT]GC
               69  GCTGC               
MboI            3  GATC                 ^GATC
              168  GATC                
MwoI          482  GCAGTGAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              598  GCACACCTTGC         
NdeII           3  GATC                 ^GATC
              168  GATC                
NlaIII        197  CATG                 CATG^
              529  CATG                
PflMI          30  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              684  CCAACTCGTGG         
Sau3AI          3  GATC                 ^GATC
              168  GATC                
SmlI          172  CTCAAG               C^T[CT][GA]AG
              799  CTTGAG              
TseI           66  GCAGC                G^C[AT]GC
               69  GCTGC               
Van91I         30  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              684  CCAACTCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AcsI        AluI        ApoI        Asp700I     BseRI       
BsiYI       BslI        BsmI        BssKI       BstF5I      
BstNI       DdeI        EcoRII      FokI        HinfI       
MaeIII      MnlI        MseI        MslI        MvaI        
ScrFI       SfcI        TfiI        Tru9I       Tsp509I     
TspRI       XmnI        
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