Restriction map for pF1KE0636
 (Linear) (Six-base) MAPSORT of: KIEE0636.seq  Check: 6122  from: 1  to: 1134



KIEE0636.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     822    1134 
   Size:        822     312
 
AflIII A'CryG_T

Cuts at:      0     780    1134 
   Size:        780     354
 
AleI CACnn'nnGTG

Cuts at:      0     934    1134 
   Size:        934     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0     241    1134 
   Size:        241     893
 
ApoI r'AATT_y

Cuts at:      0     326     620     956    1124    1134 
   Size:        326     294     336     168      10

  Fragments arranged by size: 

                 336     326     294     168      10
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     885     918    1134 
   Size:        885      33     216

  Fragments arranged by size: 

                 885     216      33
 
BanII G_rGCy'C

Cuts at:      0     232    1134 
   Size:        232     902
 
BclI T'GATC_A

Cuts at:      0       2    1134 
   Size:          2    1132
 
BfrBI ATG'CAT

Cuts at:      0     675    1134 
   Size:        675     459
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     734    1134 
   Size:        218     516     400

  Fragments arranged by size: 

                 516     400     218
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     820    1134 
   Size:        157     663     314

  Fragments arranged by size: 

                 663     314     157
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     630    1134 
   Size:        630     504
 
BsaAI yAC'GTr

Cuts at:      0     783    1134 
   Size:        783     351
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     756    1015    1134 
   Size:         73     683     259     119

  Fragments arranged by size: 

                 683     259     119      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     753    1134 
   Size:        753     381
 
BsiHKAI G_wGCw'C

Cuts at:      0     232     367    1134 
   Size:        232     135     767

  Fragments arranged by size: 

                 767     232     135
 
BsmI GAATG_Cn'

Cuts at:      0     484     493     568    1134 
   Size:        484       9      75     566

  Fragments arranged by size: 

                 566     484      75       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     232     367    1134 
   Size:        232     135     767

  Fragments arranged by size: 

                 767     232     135
 
BsrDI GCAATG_nn'

Cuts at:      0     573     657    1134 
   Size:        573      84     477

  Fragments arranged by size: 

                 573     477      84
 
BsrGI T'GTAC_A

Cuts at:      0    1113    1134 
   Size:       1113      21
 
BssSI C'ACGA_G

Cuts at:      0     223     688    1134 
   Size:        223     465     446

  Fragments arranged by size: 

                 465     446     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     488     604    1134 
   Size:        488     116     530

  Fragments arranged by size: 

                 530     488     116
 
BtgI C'CryG_G

Cuts at:      0     196    1134 
   Size:        196     938
 
BtsI GCAGTG_nn'

Cuts at:      0      50     489    1134 
   Size:         50     439     645

  Fragments arranged by size: 

                 645     439      50
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     555     590     639     674    1134 
   Size:        555      35      49      35     460

  Fragments arranged by size: 

                 555     460      49      35      35
 
EcoICRI GAG'CTC

Cuts at:      0     230    1134 
   Size:        230     904
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     734     822    1134 
   Size:        218     516      88     312

  Fragments arranged by size: 

                 516     312     218      88
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     588    1134 
   Size:        588     546
 
EcoRI G'AATT_C

Cuts at:      0     956    1134 
   Size:        956     178
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     354     386    1134 
   Size:        354      32     748

  Fragments arranged by size: 

                 748     354      32
 
HindIII A'AGCT_T

Cuts at:      0     917    1134 
   Size:        917     217
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      92    1134 
   Size:         92    1042
 
MslI CAynn'nnrTG

Cuts at:      0     136     813     934    1134 
   Size:        136     677     121     200

  Fragments arranged by size: 

                 677     200     136     121
 
MspA1I CmG'CkG

Cuts at:      0      69    1134 
   Size:         69    1065
 
NcoI C'CATG_G

Cuts at:      0     196    1134 
   Size:        196     938
 
NdeI CA'TA_TG

Cuts at:      0     907    1134 
   Size:        907     227
 
NsiI A_TGCA'T

Cuts at:      0     677    1134 
   Size:        677     457
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      36     690    1134 
   Size:         36     654     444

  Fragments arranged by size: 

                 654     444      36
 
PfoI T'CCnGG_A

Cuts at:      0      11      29    1134 
   Size:         11      18    1105

  Fragments arranged by size: 

                1105      18      11
 
PmlI CAC'GTG

Cuts at:      0     783    1134 
   Size:        783     351
 
PsiI TTA'TAA

Cuts at:      0     356     860    1134 
   Size:        356     504     274

  Fragments arranged by size: 

                 504     356     274
 
PstI C_TGCA'G

Cuts at:      0      57    1134 
   Size:         57    1077
 
PvuII CAG'CTG

Cuts at:      0      69    1134 
   Size:         69    1065
 
SacI G_AGCT'C

Cuts at:      0     232    1134 
   Size:        232     902
 
SfcI C'TryA_G

Cuts at:      0      53     120     461     585    1134 
   Size:         53      67     341     124     549

  Fragments arranged by size: 

                 549     341     124      67      53
 
SmlI C'TyrA_G

Cuts at:      0     172     799    1134 
   Size:        172     627     335

  Fragments arranged by size: 

                 627     335     172
 
StyI C'CwwG_G

Cuts at:      0     196    1134 
   Size:        196     938
 
TatI w'GTAC_w

Cuts at:      0    1113    1134 
   Size:       1113      21
 
XmnI GAAnn'nnTTC

Cuts at:      0     499     751     835    1134 
   Size:        499     252      84     299

  Fragments arranged by size: 

                 499     299     252      84
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     ApoI     BaeI    BanII     BclI
    BfrBI     BpmI    BpuEI     BsaI    BsaAI    BseRI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrDI    BsrGI    BssSI   BstAPI     BtgI     BtsI
    CspCI  EcoICRI  Eco57MI    EcoNI    EcoRI    Hin4I  HindIII     MmeI
     MslI   MspA1I     NcoI     NdeI     NsiI    PflMI     PfoI     PmlI
     PsiI     PstI    PvuII     SacI     SfcI     SmlI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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