################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AflII AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BcnI BfrI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BsrBI BsrGI BssHII Bst1107I BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI FseI FspI HincII HindII HpaI KasI KpnI KspI MluI NaeI NarI NciI NdeI NgoMIV NheI NotI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SfiI SfoI SgrAI SmaI SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccII 637 CGCG CG^CG AccIII 195 TCCGGA T^CCGGA AclI 1277 AACGTT AA^CGTT AflIII 4087 ACATGT A^C[GA][CT]GT AgeI 1470 ACCGGT A^CCGGT Alw44I 2804 GTGCAC G^TGCAC ApaLI 2804 GTGCAC G^TGCAC BalI 2 TGGCCA TGG^CCA BciVI 4405 GTATCC GTATCC(6/5) BsaAI 3091 TACGTA [CT]AC^GT[GA] BsaI 2521 GAGACC GGTCTC(1/5) BseAI 195 TCCGGA T^CCGGA BspEI 195 TCCGGA T^CCGGA BspHI 4170 TCATGA T^CATGA BspLU11I 4087 ACATGT A^CATGT BssSI 2518 CACGAG CACGAG(-5/-1) BstEII 2833 GGTTACC G^GT[ACGT]ACC BstPI 2833 GGTTACC G^GT[ACGT]ACC BstUI 637 CGCG CG^CG BstXI 6093 CCATTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoO65I 2833 GGTTACC G^GT[ACGT]ACC EcoRV 5142 GATATC GAT^ATC FauI 666 GCGGG CCCGC(4/6) HaeII 710 AGCGCC [GA]GCGC^[CT] MluNI 2 TGGCCA TGG^CCA MroI 195 TCCGGA T^CCGGA MscI 2 TGGCCA TGG^CCA MvnI 637 CGCG CG^CG NcoI 3745 CCATGG C^CATGG NruI 636 TCGCGA TCG^CGA PflMI 208 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1470 ACCGGT A^CCGGT Psp1406I 1277 AACGTT AA^CGTT RcaI 4170 TCATGA T^CATGA SbfI 4745 CCTGCAGG CCTGCA^GG ScaI 1435 AGTACT AGT^ACT SexAI 3525 ACCAGGT A^CC[AT]GGT SnaBI 3091 TACGTA TAC^GTA SpeI 5692 ACTAGT A^CTAGT Sse8387I 4745 CCTGCAGG CCTGCA^GG Van91I 208 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2793 GTCTAC GT^[AC][GT]AC 4206 GTCTAC AcyI 1108 GGCGTC G[GA]^CG[CT]C 3866 GACGCC BanI 103 GGTGCC G^G[CT][GA]CC 1467 GGCACC BbvCI 910 CCTCAGC CCTCAGC(-5/-2) 2749 CCTCAGC BclI 39 TGATCA T^GATCA 5421 TGATCA BglI 3973 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5269 GCCAAAGAGGC BsaHI 1108 GGCGTC G[GA]^CG[CT]C 3866 GACGCC BsaWI 195 TCCGGA [AT]^CCGG[AT] 1470 ACCGGT BsrFI 1470 ACCGGT [GA]^CCGG[CT] 4898 ACCGGC BstBI 1604 TTCGAA TT^CGAA 1760 TTCGAA Cfr10I 1470 ACCGGT [GA]^CCGG[CT] 4898 ACCGGC DraI 5644 TTTAAA TTT^AAA 5763 TTTAAA DsaI 896 CCACGG C^C[GA][CT]GG 3745 CCATGG EaeI 2 TGGCCA [CT]^GGCC[GA] 899 CGGCCA EcoRI 3621 GAATTC G^AATTC 4023 GAATTC EcoT22I 464 ATGCAT ATGCA^T 4388 ATGCAT FbaI 39 TGATCA T^GATCA 5421 TGATCA Hin1I 1108 GGCGTC G[GA]^CG[CT]C 3866 GACGCC MfeI 2359 CAATTG C^AATTG 2982 CAATTG MunI 2359 CAATTG C^AATTG 2982 CAATTG NsiI 464 ATGCAT ATGCA^T 4388 ATGCAT NspV 1604 TTCGAA TT^CGAA 1760 TTCGAA SacI 998 GAGCTC GAGCT^C 3082 GAGCTC SapI 2878 GAAGAGC GCTCTTC(1/4) 4132 GAAGAGC SfuI 1604 TTCGAA TT^CGAA 1760 TTCGAA SphI 2422 GCATGC GCATG^C 3047 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI