Restriction map for pF1KB5467
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcyI        
AflII       AgeI        Alw44I      AocI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspI        AviII       AvrII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfaI        
BfrI        BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        MaeI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NsiI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        PvuII       RcaI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI          565  AACGTT               AA^CGTT
AcsI          507  GAATTT               [GA]^AATT[CT]
AflIII        315  ACATGT               A^C[GA][CT]GT
AhdI          341  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI          80  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          770  GGGCCC               GGGCC^C
ApoI          507  GAATTT               [GA]^AATT[CT]
AspEI         341  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          542  TGGCCA               TGG^CCA
BamHI         755  GGATCC               G^GATCC
BanI          917  GGCACC               G^G[CT][GA]CC
BglII         836  AGATCT               A^GATCT
BlpI          264  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      264  GCTCAGC              GC^T[ACGT]AGC
BseRI         275  GAGGAG               GAGGAG(10/8)
BsiEI         309  CGGCCG               CG[GA][CT]^CG
BsmI          165  GAATGC               GAATGC(1/-1)
Bsp1407I      299  TGTACA               T^GTACA
BspLU11I      315  ACATGT               A^CATGT
BsrGI         299  TGTACA               T^GTACA
BstXI         533  CCAATGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         264  GCTCAGC              GC^T[ACGT]AGC
EagI          309  CGGCCG               C^GGCCG
Eam1105I      341  GACCTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          225  GAAGAG               CTCTTC(1/4)
EclXI         309  CGGCCG               C^GGCCG
Eco52I        309  CGGCCG               C^GGCCG
HincII        562  GTCAAC               GT[CT]^[GA]AC
HindII        562  GTCAAC               GT[CT]^[GA]AC
Ksp632I       225  GAAGAG               CTCTTC(1/4)
KspI          525  CCGCGG               CCGC^GG
MaeII         566  ACGT                 A^CGT
MluNI         542  TGGCCA               TGG^CCA
MscI          542  TGGCCA               TGG^CCA
MslI          958  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          975  CCATGG               C^CATGG
NruI          417  TCGCGA               TCG^CGA
PaeR7I         29  CTCGAG               C^TCGAG
Psp1406I      565  AACGTT               AA^CGTT
SacII         525  CCGCGG               CCGC^GG
SfiI          529  GGCCCCAATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI           29  CTCGAG               C^T[CT][GA]AG
SspBI         299  TGTACA               T^GTACA
TaqI           30  TCGA                 T^CGA
Tsp45I        748  GTCAC                ^GT[GC]AC
Tsp509I       508  AATT                 ^AATT
TthHB8I        30  TCGA                 T^CGA
XhoI           29  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI          756  GATCC                GGATC(4/5)
              755  GGATC               
Aor51HI        33  AGCGCT               AGC^GCT
              514  AGCGCT              
AspHI         413  GAGCTC               G[AT]GC[AT]^C
              673  GAGCTC              
BbvCI         849  CCTCAGC              CCTCAGC(-5/-2)
              466  GCTGAGG             
BglI          530  GCCCCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              883  GCCCACAGGGC         
BsiHKAI       413  GAGCTC               G[AT]GC[AT]^C
              673  GAGCTC              
BstF5I        213  GGATG                GGATG(2/0)
              963  GGATG               
BstYI         755  GGATCC               [GA]^GATC[CT]
              836  AGATCT              
EaeI          309  CGGCCG               [CT]^GGCC[GA]
              542  TGGCCA              
Eco47III       33  AGCGCT               AGC^GCT
              514  AGCGCT              
EcoT14I       878  CCTTGG               C^C[AT][AT]GG
              975  CCATGG              
FauI          524  CCCGC                CCCGC(4/6)
              729  CCCGC               
FokI          213  GGATG                GGATG(9/13)
              963  GGATG               
MaeIII        748  GTCAC                ^GT[ACGT]AC
              857  GTAAC               
MflI          755  GGATCC               [GA]^GATC[CT]
              836  AGATCT              
MseI          259  TTAA                 T^TAA
              605  TTAA                
MspA1I        525  CCGCGG               C[AC]G^C[GT]G
              852  CAGCGG              
NaeI          427  GCCGGC               GCC^GGC
              863  GCCGGC              
NgoMIV        427  GCCGGC               G^CCGGC
              863  GCCGGC              
NspI          315  ACATGT               [GA]CATG^[CT]
              957  GCATGT              
PpuMI         363  AGGTCCC              [GA]G^G[AT]CC[CT]
              908  GGGACCC             
PstI            8  CTGCAG               CTGCA^G
              871  CTGCAG              
SacI          413  GAGCTC               GAGCT^C
              673  GAGCTC              
SfcI            8  CTGCAG               C^T[GA][CT]AG
              871  CTGCAG              
StyI          878  CCTTGG               C^C[AT][AT]GG
              975  CCATGG              
TfiI          688  GAATC                G^A[AT]TC
              822  GAATC               
Tru9I         259  TTAA                 T^TAA
              605  TTAA                
XhoII         755  GGATCC               [GA]^GATC[CT]
              836  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BpmI        BsaJI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EcoO109I    EcoRII      Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MboI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TseI        TspRI       
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