Restriction map for pF1KB5467
 (Linear) (Six-base) MAPSORT of: KIBB5467.seq  Check: 7155  from: 1  to: 984



KIBB5467.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AclI AA'CG_TT

Cuts at:      0     566     984 
   Size:        566     418
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     775     984 
   Size:        244     531     209

  Fragments arranged by size: 

                 531     244     209
 
AfeI AGC'GCT

Cuts at:      0      35     516     984 
   Size:         35     481     468

  Fragments arranged by size: 

                 481     468      35
 
AflIII A'CryG_T

Cuts at:      0     315     984 
   Size:        315     669
 
AhdI GACnn_n'nnGTC

Cuts at:      0     346     984 
   Size:        346     638
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     664     696     984 
   Size:        664      32     288

  Fragments arranged by size: 

                 664     288      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      85     984 
   Size:         85     899
 
ApaI G_GGCC'C

Cuts at:      0     774     984 
   Size:        774     210
 
ApoI r'AATT_y

Cuts at:      0     507     984 
   Size:        507     477
 
AvaI C'yCGr_G

Cuts at:      0      29      91     922     984 
   Size:         29      62     831      62

  Fragments arranged by size: 

                 831      62      62      29
 
BamHI G'GATC_C

Cuts at:      0     755     984 
   Size:        755     229
 
BanI G'GyrC_C

Cuts at:      0     917     984 
   Size:        917      67
 
BanII G_rGCy'C

Cuts at:      0     208     417     677     718     774     984 
   Size:        208     209     260      41      56     210

  Fragments arranged by size: 

                 260     210     209     208      56      41
 
BbvCI CC'TCA_GC

Cuts at:      0     467     850     984 
   Size:        467     383     134

  Fragments arranged by size: 

                 467     383     134
 
BglI GCCn_nnn'nGGC

Cuts at:      0     536     889     984 
   Size:        536     353      95

  Fragments arranged by size: 

                 536     353      95
 
BglII A'GATC_T

Cuts at:      0     836     984 
   Size:        836     148
 
BlpI GC'TnA_GC

Cuts at:      0     265     984 
   Size:        265     719
 
Bme1580I G_kGCm'C

Cuts at:      0     774     894     984 
   Size:        774     120      90

  Fragments arranged by size: 

                 774     120      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      64     984 
   Size:         64     920
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      44     301     385     984 
   Size:         44     257      84     599

  Fragments arranged by size: 

                 599     257      84      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     293     380     691     984 
   Size:        106     187      87     311     293

  Fragments arranged by size: 

                 311     293     187     106      87
 
Bpu10I CC'TnA_GC

Cuts at:      0     467     664     814     850     984 
   Size:        467     197     150      36     134

  Fragments arranged by size: 

                 467     197     150     134      36
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     168     198     664     694     984 
   Size:        168      30     466      30     290

  Fragments arranged by size: 

                 466     290     168      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     290     984 
   Size:        290     694
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     317     493     984 
   Size:         35     282     176     491

  Fragments arranged by size: 

                 491     282     176      35
 
BsiEI CG_ry'CG

Cuts at:      0     312     984 
   Size:        312     672
 
BsiHKAI G_wGCw'C

Cuts at:      0     417     677     984 
   Size:        417     260     307

  Fragments arranged by size: 

                 417     307     260
 
BsmI GAATG_Cn'

Cuts at:      0     171     984 
   Size:        171     813
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     417     677     718     774     894     984 
   Size:        208     209     260      41      56     120      90

  Fragments arranged by size: 

                 260     209     208     120      90      56      41
 
BsrFI r'CCGG_y

Cuts at:      0     307     427     863     984 
   Size:        307     120     436     121

  Fragments arranged by size: 

                 436     307     121     120
 
BsrGI T'GTAC_A

Cuts at:      0     299     984 
   Size:        299     685
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     540     984 
   Size:        540     444
 
BstYI r'GATC_y

Cuts at:      0     755     836     984 
   Size:        755      81     148

  Fragments arranged by size: 

                 755     148      81
 
BtgI C'CryG_G

Cuts at:      0     209     525     975     984 
   Size:        209     316     450       9

  Fragments arranged by size: 

                 450     316     209       9
 
EaeI y'GGCC_r

Cuts at:      0     309     542     984 
   Size:        309     233     442

  Fragments arranged by size: 

                 442     309     233
 
EagI C'GGCC_G

Cuts at:      0     309     984 
   Size:        309     675
 
EarI CTCTTCn'nnn_

Cuts at:      0     220     984 
   Size:        220     764
 
EcoICRI GAG'CTC

Cuts at:      0     415     675     984 
   Size:        415     260     309

  Fragments arranged by size: 

                 415     309     260
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     244     293     380     691     775     984 
   Size:        106     138      49      87     311      84     209

  Fragments arranged by size: 

                 311     209     138     106      87      84      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     142     156     364     771     909     940     984 
   Size:        142      14     208     407     138      31      44

  Fragments arranged by size: 

                 407     208     142     138      44      31      14
 
HaeII r_GCGC'y

Cuts at:      0      26      37     518     933     984 
   Size:         26      11     481     415      51

  Fragments arranged by size: 

                 481     415      51      26      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     332     364     807     839     984 
   Size:        332      32     443      32     145

  Fragments arranged by size: 

                 443     332     145      32      32
 
HincII GTy'rAC

Cuts at:      0     564     984 
   Size:        564     420
 
MscI TGG'CCA

Cuts at:      0     544     984 
   Size:        544     440
 
MslI CAynn'nnrTG

Cuts at:      0     962     984 
   Size:        962      22
 
MspA1I CmG'CkG

Cuts at:      0     527     854     984 
   Size:        527     327     130

  Fragments arranged by size: 

                 527     327     130
 
NaeI GCC'GGC

Cuts at:      0     429     865     984 
   Size:        429     436     119

  Fragments arranged by size: 

                 436     429     119
 
NcoI C'CATG_G

Cuts at:      0     975     984 
   Size:        975       9
 
NgoMIV G'CCGG_C

Cuts at:      0     427     863     984 
   Size:        427     436     121

  Fragments arranged by size: 

                 436     427     121
 
NruI TCG'CGA

Cuts at:      0     419     984 
   Size:        419     565
 
NspI r_CATG'y

Cuts at:      0     319     961     984 
   Size:        319     642      23

  Fragments arranged by size: 

                 642     319      23
 
PciI A'CATG_T

Cuts at:      0     315     984 
   Size:        315     669
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     664     696     984 
   Size:        664      32     288

  Fragments arranged by size: 

                 664     288      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     364     909     984 
   Size:        364     545      75

  Fragments arranged by size: 

                 545     364      75
 
PspOMI G'GGCC_C

Cuts at:      0     770     984 
   Size:        770     214
 
PspXI vC'TCGA_Gb

Cuts at:      0      29     984 
   Size:         29     955
 
PstI C_TGCA'G

Cuts at:      0      12     875     984 
   Size:         12     863     109

  Fragments arranged by size: 

                 863     109      12
 
SacI G_AGCT'C

Cuts at:      0     417     677     984 
   Size:        417     260     307

  Fragments arranged by size: 

                 417     307     260
 
SacII CC_GC'GG

Cuts at:      0     528     984 
   Size:        528     456
 
SanDI GG'GwC_CC

Cuts at:      0     909     984 
   Size:        909      75
 
SfcI C'TryA_G

Cuts at:      0       8     871     984 
   Size:          8     863     113

  Fragments arranged by size: 

                 863     113       8
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     536     984 
   Size:        536     448
 
SmlI C'TyrA_G

Cuts at:      0      29     984 
   Size:         29     955
 
StyI C'CwwG_G

Cuts at:      0     878     975     984 
   Size:        878      97       9

  Fragments arranged by size: 

                 878      97       9
 
TatI w'GTAC_w

Cuts at:      0     299     984 
   Size:        299     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151     168     200     984 
   Size:        119      32      17      32     784

  Fragments arranged by size: 

                 784     119      32      32      17
 
XhoI C'TCGA_G

Cuts at:      0      29     984 
   Size:         29     955
 

 Enzymes that do cut:

     AclI     AcuI     AfeI   AflIII     AhdI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII    BbvCI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BsrFI    BsrGI    BstXI
    BstYI     BtgI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NruI     NspI     PciI     PpiI    PpuMI   PspOMI    PspXI
     PstI     SacI    SacII    SanDI     SfcI     SfiI     SmlI     StyI
     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII     AgeI     AleI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NdeI     NheI     NotI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI
    PvuII    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI
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