Restriction map for
pF1KB5360
(Circular) (Six-base) MAPSORT of: pF1KB5360.seq Check: 3637 from: 1 to: 4217
pF1KB5360 4217 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 1255 1255
Size: 4217
Acc65I G'GTAC_C
Cuts at: 1233 1233
Size: 4217
AclI AA'CG_TT
Cuts at: 4057 4057
Size: 4217
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 893 2042 2474 3221 3489 893
Size: 1149 432 747 268 1621
Fragments arranged by size:
1621 1149 747 432 268
AflIII A'CryG_T
Cuts at: 1569 2673 3650 1569
Size: 1104 977 2136
Fragments arranged by size:
2136 1104 977
AgeI A'CCGG_T
Cuts at: 2605 2605
Size: 4217
AlwNI CAG_nnn'CTG
Cuts at: 1004 1522 3089 1004
Size: 518 1567 2132
Fragments arranged by size:
2132 1567 518
ApaLI G'TGCA_C
Cuts at: 2987 2987
Size: 4217
ApoI r'AATT_y
Cuts at: 715 1135 1221 3570 3826 715
Size: 420 86 2349 256 1106
Fragments arranged by size:
2349 1106 420 256 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 4217
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
AvaI C'yCGr_G
Cuts at: 1237 1237
Size: 4217
BamHI G'GATC_C
Cuts at: 1242 1242
Size: 4217
BanI G'GyrC_C
Cuts at: 964 1233 1647 1895 1930 964
Size: 269 414 248 35 3251
Fragments arranged by size:
3251 414 269 248 35
BanII G_rGCy'C
Cuts at: 1231 2261 1231
Size: 1030 3187
BbeI G_GCGC'C
Cuts at: 1651 1899 1651
Size: 248 3969
BbvCI CC'TCA_GC
Cuts at: 451 451
Size: 4217
BciVI GTATCCnnnnn_n'
Cuts at: 570 2115 2876 4217 570
Size: 1545 761 1341 570
Fragments arranged by size:
1545 1341 761 570
BclI T'GATC_A
Cuts at: 1737 1737
Size: 4217
BglII A'GATC_T
Cuts at: 1732 3769 1732
Size: 2037 2180
BlpI GC'TnA_GC
Cuts at: 1329 1329
Size: 4217
Bme1580I G_kGCm'C
Cuts at: 1842 1935 2991 1842
Size: 93 1056 3068
Fragments arranged by size:
3068 1056 93
BmgBI CAC'GTC
Cuts at: 639 639
Size: 4217
BmrI ACTGGGnnnn_n'
Cuts at: 374 554 1429 1510 1845 3989 374
Size: 180 875 81 335 2144 602
Fragments arranged by size:
2144 875 602 335 180 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2584 2584
Size: 4217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 4217
Bpu10I CC'TnA_GC
Cuts at: 451 451
Size: 4217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1393 2272 2764 3062 1393
Size: 879 492 298 2548
Fragments arranged by size:
2548 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3921 3921
Size: 4217
BsaAI yAC'GTr
Cuts at: 1570 1570
Size: 4217
BsaBI GATnn'nnATC
Cuts at: 6 472 1731 3768 6
Size: 466 1259 2037 455
Fragments arranged by size:
2037 1259 466 455
BsaHI Gr'CG_yC
Cuts at: 1648 1896 2598 4094 1648
Size: 248 702 1496 1771
Fragments arranged by size:
1771 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1406 1927 2605 2879 3026 3437 1406
Size: 521 678 274 147 411 2186
Fragments arranged by size:
2186 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 201 231 897 927 201
Size: 30 666 30 3491
Fragments arranged by size:
3491 666 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 905 905
Size: 4217
BseYI C'CCAG_C
Cuts at: 311 350 551 1642 2400 2977 3473 311
Size: 39 201 1091 758 577 496 1055
Fragments arranged by size:
1091 1055 758 577 496 201 39
BsiEI CG_ry'CG
Cuts at: 104 586 1759 1805 3013 104
Size: 482 1173 46 1208 1308
Fragments arranged by size:
1308 1208 1173 482 46
BsiHKAI G_wGCw'C
Cuts at: 1231 2009 2199 2991 1231
Size: 778 190 792 2457
Fragments arranged by size:
2457 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1231 1842 1935 2009 2199 2261 2991 1231
Size: 611 93 74 190 62 730 2457
Fragments arranged by size:
2457 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3437 3437
Size: 4217
BspHI T'CATG_A
Cuts at: 3393 3393
Size: 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1267 1481 1783 2164 1267
Size: 214 302 381 3320
Fragments arranged by size:
3320 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2509 2563 4047 4212 50
Size: 2459 54 1484 165 55
Fragments arranged by size:
2459 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2129 2129
Size: 4217
BsrFI r'CCGG_y
Cuts at: 2215 2396 2605 2215
Size: 181 209 3827
Fragments arranged by size:
3827 209 181
BssSI C'ACGA_G
Cuts at: 2488 2846 2488
Size: 358 3859
BstAPI GCAn_nnn'nTGC
Cuts at: 1594 1594
Size: 4217
BstBI TT'CG_AA
Cuts at: 2578 2578
Size: 4217
BstXI CCAn_nnnn'nTGG
Cuts at: 657 657
Size: 4217
BstYI r'GATC_y
Cuts at: 1 982 1242 1709 1732 2067 2313 3325 3411
Size: 981 260 467 23 335 246 1012 86
Cuts at: 3411 3423 3769 1
Size: 12 346 449
Fragments arranged by size:
1012 981 467 449 346 335 260 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 129 917 129
Size: 788 3429
BtgI C'CryG_G
Cuts at: 650 650
Size: 4217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2167 2348 2511 2167
Size: 181 163 3873
Fragments arranged by size:
3873 181 163
BtsI GCAGTG_nn'
Cuts at: 171 1460 171
Size: 1289 2928
ClaI AT'CG_AT
Cuts at: 888 888
Size: 4217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 242 277 242
Size: 35 4182
DraI TTT'AAA
Cuts at: 566 771 879 1216 566
Size: 205 108 337 3567
Fragments arranged by size:
3567 337 205 108
DrdI GACnn_nn'nnGTC
Cuts at: 1923 2781 1923
Size: 858 3359
EaeI y'GGCC_r
Cuts at: 1802 1976 2367 2394 3739 1802
Size: 174 391 27 1345 2280
Fragments arranged by size:
2280 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1802 1802
Size: 4217
EarI CTCTTCn'nnn_
Cuts at: 1181 2240 2450 1181
Size: 1059 210 2948
Fragments arranged by size:
2948 1059 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 2735 2881 4027 2425
Size: 310 146 1146 2615
Fragments arranged by size:
2615 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1229 1229
Size: 4217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 893 2042 2474 3221 3489 408
Size: 485 1149 432 747 268 1136
Fragments arranged by size:
1149 1136 747 485 432 268
EcoO109I rG'GnC_Cy
Cuts at: 447 678 1356 447
Size: 231 678 3308
Fragments arranged by size:
3308 678 231
EcoRI G'AATT_C
Cuts at: 715 1221 715
Size: 506 3711
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 4217
FspI TGC'GCA
Cuts at: 1998 1998
Size: 4217
HaeII r_GCGC'y
Cuts at: 1651 1899 2921 3900 1651
Size: 248 1022 979 1968
Fragments arranged by size:
1968 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 504 536 568 600 675 707 717 749 2059
Size: 32 32 32 75 32 10 32 1310
Cuts at: 2059 2089 2091 2121 504
Size: 30 2 30 2600
Fragments arranged by size:
2600 1310 75 32 32 32 32 32
30 30 10 2
HincII GTy'rAC
Cuts at: 1256 1256
Size: 4217
KasI G'GCGC_C
Cuts at: 1647 1895 1647
Size: 248 3969
KpnI G_GTAC'C
Cuts at: 1237 1237
Size: 4217
MluI A'CGCG_T
Cuts at: 3650 3650
Size: 4217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2888 3072 2888
Size: 184 4033
MscI TGG'CCA
Cuts at: 1978 1978
Size: 4217
MslI CAynn'nnrTG
Cuts at: 655 755 2206 2333 655
Size: 100 1451 127 2539
Fragments arranged by size:
2539 1451 127 100
MspA1I CmG'CkG
Cuts at: 533 1328 1642 2002 3015 3260 533
Size: 795 314 360 1013 245 1490
Fragments arranged by size:
1490 1013 795 360 314 245
NaeI GCC'GGC
Cuts at: 2398 2398
Size: 4217
NarI GG'CG_CC
Cuts at: 1648 1896 1648
Size: 248 3969
NcoI C'CATG_G
Cuts at: 650 650
Size: 4217
NgoMIV G'CCGG_C
Cuts at: 2396 2396
Size: 4217
NspI r_CATG'y
Cuts at: 159 1270 1770 2677 159
Size: 1111 500 907 1699
Fragments arranged by size:
1699 1111 907 500
PasI CC'CwG_GG
Cuts at: 804 804
Size: 4217
PciI A'CATG_T
Cuts at: 2673 2673
Size: 4217
PflMI CCAn_nnn'nTGG
Cuts at: 443 509 443
Size: 66 4151
PmeI GTTT'AAAC
Cuts at: 1216 1216
Size: 4217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3388 3356
Size: 32 4185
PpuMI rG'GwC_Cy
Cuts at: 447 447
Size: 4217
PsiI TTA'TAA
Cuts at: 1142 1142
Size: 4217
PstI C_TGCA'G
Cuts at: 672 1264 1949 672
Size: 592 685 2940
Fragments arranged by size:
2940 685 592
PvuI CG_AT'CG
Cuts at: 104 586 104
Size: 482 3735
PvuII CAG'CTG
Cuts at: 533 1642 2002 533
Size: 1109 360 2748
Fragments arranged by size:
2748 1109 360
RsrII CG'GwC_CG
Cuts at: 2412 2412
Size: 4217
SacI G_AGCT'C
Cuts at: 1231 1231
Size: 4217
SalI G'TCGA_C
Cuts at: 1254 1254
Size: 4217
SapI GCTCTTCn'nnn_
Cuts at: 1181 2240 2450 1181
Size: 1059 210 2948
Fragments arranged by size:
2948 1059 210
SbfI CC_TGCA'GG
Cuts at: 1264 1264
Size: 4217
ScaI AGT'ACT
Cuts at: 3734 3734
Size: 4217
SfcI C'TryA_G
Cuts at: 33 173 528 668 1260 1945 2938 3129 33
Size: 140 355 140 592 685 993 191 1121
Fragments arranged by size:
1121 993 685 592 355 191 140 140
SfoI GGC'GCC
Cuts at: 1649 1897 1649
Size: 248 3969
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4217
SmaI CCC'GGG
Cuts at: 1239 1239
Size: 4217
SmlI C'TyrA_G
Cuts at: 1372 2287 2779 3041 1372
Size: 915 492 262 2548
Fragments arranged by size:
2548 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4217
SphI G_CATG'C
Cuts at: 1270 1770 1270
Size: 500 3717
StyI C'CwwG_G
Cuts at: 650 1351 1705 650
Size: 701 354 3162
Fragments arranged by size:
3162 701 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1800 1933 2392 2601 1800
Size: 133 459 209 3416
Fragments arranged by size:
3416 459 209 133
TatI w'GTAC_w
Cuts at: 3732 3732
Size: 4217
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 897 929 3734 3766 897
Size: 32 2805 32 1348
Fragments arranged by size:
2805 1348 32 32
Tth111I GACn'n_nGTC
Cuts at: 2014 2014
Size: 4217
XbaI T'CTAG_A
Cuts at: 1248 1248
Size: 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 657 958 1712 657
Size: 301 754 3162
Fragments arranged by size:
3162 754 301
XmaI C'CCGG_G
Cuts at: 1237 1237
Size: 4217
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI