Restriction map for pF1KB5294
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# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvrII       BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspEI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstYI       
BstZ17I     CelII       CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HaeII       HindIII     KasI        
KpnI        KspI        MaeI        MfeI        MflI        
MluI        MroI        MslI        MspA1I      MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp509I     Tth111I     XbaI        XcmI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccII         565  CGCG                 CG^CG
AclI          444  AACGTT               AA^CGTT
AcyI           83  GACGCC               G[GA]^CG[CT]C
AflIII        881  ACACGT               A^C[GA][CT]GT
AocI          793  CCTGAGG              CC^T[ACGT]AGG
AvaI          384  CTCGAG               C^[CT]CG[GA]G
AvaII         396  GGACC                G^G[AT]CC
AviII         803  TGCGCA               TGC^GCA
BanII         709  GAGCCC               G[GA]GC[CT]^C
BcgI          716  GCAACTTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI        1017  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          83  GACGCC               G[GA]^CG[CT]C
BseRI         784  GAGGAG               GAGGAG(10/8)
BsmFI         885  GTCCC                GGGAC(10/14)
BsoBI         384  CTCGAG               C^[CT]CG[GA]G
BspDI         895  ATCGAT               AT^CGAT
BspHI         983  TCATGA               T^CATGA
BspMI         486  GCAGGT               ACCTGC(4/8)
BstUI         565  CGCG                 CG^CG
BstXI         990  CCACTTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        793  CCTGAGG              CC^T[ACGT]AGG
ClaI          895  ATCGAT               AT^CGAT
DsaI          950  CCATGG               C^C[GA][CT]GG
EarI          352  GAAGAG               CTCTTC(1/4)
Eco81I        793  CCTGAGG              CC^T[ACGT]AGG
EcoNI         400  CCTGAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       950  CCATGG               C^C[AT][AT]GG
FspI          803  TGCGCA               TGC^GCA
Hin1I          83  GACGCC               G[GA]^CG[CT]C
HincII        424  GTTAAC               GT[CT]^[GA]AC
HindII        424  GTTAAC               GT[CT]^[GA]AC
HinfI         190  GACTC                G^A[ACGT]TC
HpaI          424  GTTAAC               GTT^AAC
Ksp632I       352  GAAGAG               CTCTTC(1/4)
MamI         1017  GATCTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          425  TTAA                 T^TAA
MvnI          565  CGCG                 CG^CG
NaeI          433  GCCGGC               GCC^GGC
NcoI          950  CCATGG               C^CATGG
NgoMIV        433  GCCGGC               G^CCGGC
PaeR7I        384  CTCGAG               C^TCGAG
PflMI         945  CCACCCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          190  GACTC                GAGTC(4/5)
PpuMI         395  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI          23  GACCCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      444  AACGTT               AA^CGTT
RcaI          983  TCATGA               T^CATGA
ScaI          824  AGTACT               AGT^ACT
SfiI          600  GGCCAACCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          384  CTCGAG               C^T[CT][GA]AG
SphI          584  GCATGC               GCATG^C
StyI          950  CCATGG               C^C[AT][AT]GG
Tru9I         425  TTAA                 T^TAA
Van91I        945  CCACCCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          384  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BcnI          606  CCCGG                CC^[GC]GG
              712  CCCGG               
BglI          139  GCCAGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              601  GCCAACCCGGC         
BmyI          510  GGGCAC               G[AGT]GC[ACT]^C
              709  GAGCCC              
BsaI          475  GAGACC               GGTCTC(1/5)
              853  GAGACC              
BsmAI         475  GAGAC                GTCTC(1/5)
              853  GAGAC               
Bsp1286I      510  GGGCAC               G[AGT]GC[ACT]^C
              709  GAGCCC              
BsrBI         381  CCGCTC               CCGCTC(-3/-3)
              612  CCGCTC              
BsrFI         153  GCCGGT               [GA]^CCGG[CT]
              433  GCCGGC              
BsrI          962  ACTGG                ACTGG(1/-1)
              140  CCAGT               
CfoI          666  GCGC                 GCG^C
              804  GCGC                
Cfr10I        153  GCCGGT               [GA]^CCGG[CT]
              433  GCCGGC              
DraII         395  AGGACCC              [GA]G^G[ACGT]CC[CT]
              416  GGGGCCT             
EcoO109I      395  AGGACCC              [GA]G^G[ACGT]CC[CT]
              416  GGGGCCT             
HgaI           83  GACGC                GACGC(5/10)
              566  GCGTC               
HhaI          666  GCGC                 GCG^C
              804  GCGC                
HinP1I        666  GCGC                 G^CGC
              804  GCGC                
HphI          464  TCACC                GGTGA(8/7)
              629  TCACC               
MaeII         445  ACGT                 A^CGT
              883  ACGT                
MboII         352  GAAGA                GAAGA(8/7)
              774  GAAGA               
NciI          606  CCCGG                CC^[GC]GG
              712  CCCGG               
NspI          410  GCATGT               [GA]CATG^[CT]
              584  GCATGC              
PstI          342  CTGCAG               CTGCA^G
              861  CTGCAG              
SfcI          342  CTGCAG               C^T[GA][CT]AG
              861  CTGCAG              
TaqI          385  TCGA                 T^CGA
              896  TCGA                
TthHB8I       385  TCGA                 T^CGA
              896  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BbvI        
BpmI        BsaJI       BsiYI       BslI        BsmI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MaeIII      MboI        MluNI       MnlI        
MscI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      TspRI       
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