Restriction map for pF1KB5294
 (Linear) (Six-base) MAPSORT of: KIBB5294.seq  Check: 7190  from: 1  to: 1029



KIBB5294.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     477    1029 
   Size:        477     552
 
AclI AA'CG_TT

Cuts at:      0     445    1029 
   Size:        445     584
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    1029 
   Size:        412     617
 
AflIII A'CryG_T

Cuts at:      0     881    1029 
   Size:        881     148
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     445     477    1029 
   Size:        445      32     552

  Fragments arranged by size: 

                 552     445      32
 
AvaI C'yCGr_G

Cuts at:      0     384    1029 
   Size:        384     645
 
BanII G_rGCy'C

Cuts at:      0     713    1029 
   Size:        713     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     705     739    1029 
   Size:        705      34     290

  Fragments arranged by size: 

                 705     290      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     145     607    1029 
   Size:        145     462     422

  Fragments arranged by size: 

                 462     422     145
 
Bme1580I G_kGCm'C

Cuts at:      0     514    1029 
   Size:        514     515
 
BmgBI CAC'GTC

Cuts at:      0     884    1029 
   Size:        884     145
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     669    1029 
   Size:        669     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     493    1019    1029 
   Size:          3     490     526      10

  Fragments arranged by size: 

                 526     490      10       3
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     469     847    1029 
   Size:        469     378     182

  Fragments arranged by size: 

                 469     378     182
 
BsaBI GATnn'nnATC

Cuts at:      0    1021    1029 
   Size:       1021       8
 
BsaHI Gr'CG_yC

Cuts at:      0      84    1029 
   Size:         84     945
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475    1029 
   Size:        445      30     554

  Fragments arranged by size: 

                 554     445      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     799    1029 
   Size:        799     230
 
BseYI C'CCAG_C

Cuts at:      0     588    1029 
   Size:        588     441
 
BsmI GAATG_Cn'

Cuts at:      0     225     755     980    1029 
   Size:        225     530     225      49

  Fragments arranged by size: 

                 530     225     225      49
 
Bsp1286I G_dGCh'C

Cuts at:      0     514     713    1029 
   Size:        514     199     316

  Fragments arranged by size: 

                 514     316     199
 
BspHI T'CATG_A

Cuts at:      0     983    1029 
   Size:        983      46
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     477    1029 
   Size:        477     552
 
BsrBI CCG'CTC

Cuts at:      0     383     614    1029 
   Size:        383     231     415

  Fragments arranged by size: 

                 415     383     231
 
BsrFI r'CCGG_y

Cuts at:      0     153     433    1029 
   Size:        153     280     596

  Fragments arranged by size: 

                 596     280     153
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     997    1029 
   Size:        997      32
 
Bsu36I CC'TnA_GG

Cuts at:      0     794    1029 
   Size:        794     235
 
BtgI C'CryG_G

Cuts at:      0     950    1029 
   Size:        950      79
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     708     750    1029 
   Size:        708      42     279

  Fragments arranged by size: 

                 708     279      42
 
BtsI GCAGTG_nn'

Cuts at:      0     873    1029 
   Size:        873     156
 
ClaI AT'CG_AT

Cuts at:      0     896    1029 
   Size:        896     133
 
EaeI y'GGCC_r

Cuts at:      0       5     123     212     599    1029 
   Size:          5     118      89     387     430

  Fragments arranged by size: 

                 430     387     118      89       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     347    1029 
   Size:        347     682
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     163    1029 
   Size:        163     866
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       3     412     493    1019    1029 
   Size:          3     409      81     526      10

  Fragments arranged by size: 

                 526     409      81      10       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     404    1029 
   Size:        404     625
 
EcoO109I rG'GnC_Cy

Cuts at:      0     396     417    1029 
   Size:        396      21     612

  Fragments arranged by size: 

                 612     396      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     256     520     843    1029 
   Size:        256     264     323     186

  Fragments arranged by size: 

                 323     264     256     186
 
FspI TGC'GCA

Cuts at:      0     805    1029 
   Size:        805     224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     111     143    1029 
   Size:        111      32     886

  Fragments arranged by size: 

                 886     111      32
 
HincII GTy'rAC

Cuts at:      0     426    1029 
   Size:        426     603
 
HpaI GTT'AAC

Cuts at:      0     426    1029 
   Size:        426     603
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     109    1029 
   Size:        109     920
 
MscI TGG'CCA

Cuts at:      0       7     214     601    1029 
   Size:          7     207     387     428

  Fragments arranged by size: 

                 428     387     207       7
 
NaeI GCC'GGC

Cuts at:      0     435    1029 
   Size:        435     594
 
NcoI C'CATG_G

Cuts at:      0     950    1029 
   Size:        950      79
 
NgoMIV G'CCGG_C

Cuts at:      0     433    1029 
   Size:        433     596
 
NspI r_CATG'y

Cuts at:      0     414     588    1029 
   Size:        414     174     441

  Fragments arranged by size: 

                 441     414     174
 
PasI CC'CwG_GG

Cuts at:      0     684    1029 
   Size:        684     345
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     951    1029 
   Size:        951      78
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477    1029 
   Size:        445      32     552

  Fragments arranged by size: 

                 552     445      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     396    1029 
   Size:        396     633
 
PshAI GACnn'nnGTC

Cuts at:      0      27    1029 
   Size:         27    1002
 
PspXI vC'TCGA_Gb

Cuts at:      0     384    1029 
   Size:        384     645
 
PstI C_TGCA'G

Cuts at:      0     346     865    1029 
   Size:        346     519     164

  Fragments arranged by size: 

                 519     346     164
 
ScaI AGT'ACT

Cuts at:      0     826    1029 
   Size:        826     203
 
SfcI C'TryA_G

Cuts at:      0     342     861    1029 
   Size:        342     519     168

  Fragments arranged by size: 

                 519     342     168
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     607    1029 
   Size:        607     422
 
SmlI C'TyrA_G

Cuts at:      0     384    1029 
   Size:        384     645
 
SphI G_CATG'C

Cuts at:      0     588    1029 
   Size:        588     441
 
StyI C'CwwG_G

Cuts at:      0     950    1029 
   Size:        950      79
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     329    1029 
   Size:        329     700
 
TatI w'GTAC_w

Cuts at:      0     824    1029 
   Size:        824     205
 
XhoI C'TCGA_G

Cuts at:      0     384    1029 
   Size:        384     645
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AloI     AvaI    BanII     BcgI
     BglI Bme1580I    BmgBI     BplI     BpmI     BsaI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI     FspI    Hin4I
   HincII     HpaI     MmeI     MscI     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PpiI    PpuMI    PshAI    PspXI     PstI     ScaI
     SfcI     SfiI     SmlI     SphI     StyI    TaqII     TatI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI    BglII     BlpI     BmrI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI
    EcoRV     FseI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MslI   MspA1I     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PsiI   PspOMI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com