Restriction map for pF1KB9261
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HincII      HindII      HindIII     
HinfI       HpaI        KpnI        Ksp632I     MaeI        
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          241  GTAC                 GT^AC
AlwI          225  GATCC                GGATC(4/5)
ApaI            5  GGGCCC               GGGCC^C
AvaI          389  CCCGGG               C^[CT]CG[GA]G
BanII           5  GGGCCC               G[GA]GC[CT]^C
BbsI          310  GAAGAC               GAAGAC(2/6)
BbvCI         573  GCTGAGG              CCTCAGC(-5/-2)
BmyI            5  GGGCCC               G[AGT]GC[ACT]^C
BpmI          220  CTGGAG               CTGGAG(16/14)
BpuAI         310  GAAGAC               GAAGAC(2/6)
BsgI          487  GTGCAG               GTGCAG(16/14)
BsmBI         108  CGTCTC               CGTCTC(1/5)
BsoBI         389  CCCGGG               C^[CT]CG[GA]G
Bsp1286I        5  GGGCCC               G[AGT]GC[ACT]^C
BspMI         479  ACCTGC               ACCTGC(4/8)
BsrBI          10  CCGCTC               CCGCTC(-3/-3)
BsrFI          82  GCCGGC               [GA]^CCGG[CT]
BstYI         224  AGATCC               [GA]^GATC[CT]
Cfr10I         82  GCCGGC               [GA]^CCGG[CT]
DpnII         225  GATC                 ^GATC
DrdI          100  GACAACCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HphI          393  GGTGA                GGTGA(8/7)
KspI          192  CCGCGG               CCGC^GG
MboI          225  GATC                 ^GATC
MboII         310  GAAGA                GAAGA(8/7)
MflI          224  AGATCC               [GA]^GATC[CT]
NaeI           82  GCCGGC               GCC^GGC
NdeII         225  GATC                 ^GATC
NgoMIV         82  GCCGGC               G^CCGGC
PflMI          36  CCACTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          603  CTGCAG               CTGCA^G
RsaI          241  GTAC                 GT^AC
SacII         192  CCGCGG               CCGC^GG
Sau3AI        225  GATC                 ^GATC
SfcI          603  CTGCAG               C^T[GA][CT]AG
SmaI          389  CCCGGG               CCC^GGG
TspRI         229  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I         36  CCACTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          332  CCAAAATAAGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         224  AGATCC               [GA]^GATC[CT]
XmaCI         389  CCCGGG               C^CCGGG
XmaI          389  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           28  GGCGCC               G[GA]^CG[CT]C
               67  GGCGCC              
Aor51HI        21  AGCGCT               AGC^GCT
              581  AGCGCT              
AvaII         260  GGACC                G^G[AT]CC
              591  GGACC               
BbeI           28  GGCGCC               GGCGC^C
               67  GGCGCC              
BsaHI          28  GGCGCC               G[GA]^CG[CT]C
               67  GGCGCC              
BsmAI         133  GAGAC                GTCTC(1/5)
              109  GTCTC               
BsrI          257  ACTGG                ACTGG(1/-1)
              228  CCAGT               
DdeI          302  CTCAG                C^T[ACGT]AG
              574  CTGAG               
DraII           4  GGGGCCC              [GA]G^G[ACGT]CC[CT]
                5  GGGCCCC             
DsaI          192  CCGCGG               C^C[GA][CT]GG
              401  CCACGG              
EaeI           84  CGGCCA               [CT]^GGCC[GA]
              114  CGGCCA              
Eco47III       21  AGCGCT               AGC^GCT
              581  AGCGCT              
EcoO109I        4  GGGGCCC              [GA]G^G[ACGT]CC[CT]
                5  GGGCCCC             
HgaI          313  GACGC                GACGC(5/10)
              428  GCGTC               
Hin1I          28  GGCGCC               G[GA]^CG[CT]C
               67  GGCGCC              
KasI           28  GGCGCC               G^GCGCC
               67  GGCGCC              
MaeII         107  ACGT                 A^CGT
              170  ACGT                
MspA1I        192  CCGCGG               C[AC]G^C[GT]G
              198  CAGCGG              
NarI           28  GGCGCC               GG^CGCC
               67  GGCGCC              
NlaIII        142  CATG                 CATG^
              147  CATG                
SfaNI         298  GCATC                GCATC(5/9)
              513  GCATC               
SfoI           28  GGCGCC               GGC^GCC
               67  GGCGCC              
StuI          473  AGGCCT               AGG^CCT
              599  AGGCCT              
TaqI          283  TCGA                 T^CGA
              434  TCGA                
TthHB8I       283  TCGA                 T^CGA
              434  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AccII       AciI        AluI        BanI        BbvI        
BcnI        BsaJI       BsiYI       BslI        BsmFI       
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIV       Sau96I      ScrFI       
TseI        
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