Restriction map for pF1KB9261
 (Linear) (Six-base) MAPSORT of: KIBB9261.seq  Check: 284  from: 1  to: 612



KIBB9261.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     612 
   Size:        423     189
 
AfeI AGC'GCT

Cuts at:      0      23     583     612 
   Size:         23     560      29

  Fragments arranged by size: 

                 560      29      23
 
ApaI G_GGCC'C

Cuts at:      0       9     612 
   Size:          9     603
 
AvaI C'yCGr_G

Cuts at:      0     389     612 
   Size:        389     223
 
BanI G'GyrC_C

Cuts at:      0      28      67     412     458     612 
   Size:         28      39     345      46     154

  Fragments arranged by size: 

                 345     154      46      39      28
 
BanII G_rGCy'C

Cuts at:      0       9     612 
   Size:          9     603
 
BbeI G_GCGC'C

Cuts at:      0      32      71     612 
   Size:         32      39     541

  Fragments arranged by size: 

                 541      39      32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     317     612 
   Size:        317     295
 
BbvCI CC'TCA_GC

Cuts at:      0     574     612 
   Size:        574      38
 
Bme1580I G_kGCm'C

Cuts at:      0       9     612 
   Size:          9     603
 
BmgBI CAC'GTC

Cuts at:      0     108     171     612 
   Size:        108      63     441

  Fragments arranged by size: 

                 441     108      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     612 
   Size:        241     371
 
Bpu10I CC'TnA_GC

Cuts at:      0     574     612 
   Size:        574      38
 
BsaHI Gr'CG_yC

Cuts at:      0      29      68     612 
   Size:         29      39     544

  Fragments arranged by size: 

                 544      39      29
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      94     124     214     244     612 
   Size:         94      30      90      30     368

  Fragments arranged by size: 

                 368      94      90      30      30
 
BseYI C'CCAG_C

Cuts at:      0      24     612 
   Size:         24     588
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     612 
   Size:        508     104
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     114     612 
   Size:        114     498
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     612 
   Size:          9     603
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     488     612 
   Size:        488     124
 
BsrBI CCG'CTC

Cuts at:      0      12     612 
   Size:         12     600
 
BsrFI r'CCGG_y

Cuts at:      0      82     612 
   Size:         82     530
 
BstYI r'GATC_y

Cuts at:      0     224     612 
   Size:        224     388
 
BtgI C'CryG_G

Cuts at:      0     192     401     612 
   Size:        192     209     211

  Fragments arranged by size: 

                 211     209     192
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     106     612 
   Size:        106     506
 
EaeI y'GGCC_r

Cuts at:      0      84     114     612 
   Size:         84      30     498

  Fragments arranged by size: 

                 498      84      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     423     612 
   Size:        241     182     189

  Fragments arranged by size: 

                 241     189     182
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5       6     612 
   Size:          5       1     606

  Fragments arranged by size: 

                 606       5       1
 
HaeII r_GCGC'y

Cuts at:      0      25      32      71     585     612 
   Size:         25       7      39     514      27

  Fragments arranged by size: 

                 514      39      27      25       7
 
KasI G'GCGC_C

Cuts at:      0      28      67     612 
   Size:         28      39     545

  Fragments arranged by size: 

                 545      39      28
 
MspA1I CmG'CkG

Cuts at:      0     194     200     612 
   Size:        194       6     412

  Fragments arranged by size: 

                 412     194       6
 
NaeI GCC'GGC

Cuts at:      0      84     612 
   Size:         84     528
 
NarI GG'CG_CC

Cuts at:      0      29      68     612 
   Size:         29      39     544

  Fragments arranged by size: 

                 544      39      29
 
NgoMIV G'CCGG_C

Cuts at:      0      82     612 
   Size:         82     530
 
PasI CC'CwG_GG

Cuts at:      0      95     612 
   Size:         95     517
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42     612 
   Size:         42     570
 
PspOMI G'GGCC_C

Cuts at:      0       5     612 
   Size:          5     607
 
PstI C_TGCA'G

Cuts at:      0     607     612 
   Size:        607       5
 
SacII CC_GC'GG

Cuts at:      0     195     612 
   Size:        195     417
 
SfcI C'TryA_G

Cuts at:      0     603     612 
   Size:        603       9
 
SfoI GGC'GCC

Cuts at:      0      30      69     612 
   Size:         30      39     543

  Fragments arranged by size: 

                 543      39      30
 
SmaI CCC'GGG

Cuts at:      0     391     612 
   Size:        391     221
 
StuI AGG'CCT

Cuts at:      0     475     601     612 
   Size:        475     126      11

  Fragments arranged by size: 

                 475     126      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     277     612 
   Size:        277     335
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     214     246     612 
   Size:        214      32     366

  Fragments arranged by size: 

                 366     214      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339     612 
   Size:        339     273
 
XmaI C'CCGG_G

Cuts at:      0     389     612 
   Size:        389     223
 

 Enzymes that do cut:

     AcuI     AfeI     ApaI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI Bme1580I    BmgBI     BpmI   Bpu10I    BsaHI    BsaXI    BseYI
     BsgI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BstYI     BtgI
     DrdI     EaeI  Eco57MI EcoO109I    HaeII     KasI   MspA1I     NaeI
     NarI   NgoMIV     PasI    PflMI   PspOMI     PstI    SacII     SfcI
     SfoI     SmaI     StuI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com