Restriction map for pF1KB6668
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcsI        
AflII       AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspHI       AvaI        
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BciVI       BclI        BcnI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HhaI        HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StyI        SwaI        Van91I      XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AclI          176  AACGTT               AA^CGTT
AfaI          992  GTAC                 GT^AC
AflIII         54  ACATGT               A^C[GA][CT]GT
AhdI          728  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1101  GATCC                GGATC(4/5)
AspEI         728  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          477  GGCACC               G^G[CT][GA]CC
BcgI           15  GCAGGGTTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          882  GCCAGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1063  CTCCAG               CTGGAG(16/14)
BsaI         1035  GGTCTC               GGTCTC(1/5)
BsgI         1308  GTGCAG               GTGCAG(16/14)
BspLU11I       54  ACATGT               A^CATGT
BspMI          11  ACCTGC               ACCTGC(4/8)
BstEII        453  GGTGACC              G^GT[ACGT]ACC
BstPI         453  GGTGACC              G^GT[ACGT]ACC
BstXI         524  CCATCATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         289  GCTGGC               GC[ACGT]^[ACGT]GC
DraI          165  TTTAAA               TTT^AAA
Eam1105I      728  GACAAGGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       453  GGTGACC              G^GT[ACGT]ACC
EcoT22I       275  ATGCAT               ATGCA^T
MaeII         177  ACGT                 A^CGT
MslI          829  CACAGCTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1216  CCGCTG               C[AC]G^C[GT]G
NsiI          275  ATGCAT               ATGCA^T
NspI           54  ACATGT               [GA]CATG^[CT]
Psp1406I      176  AACGTT               AA^CGTT
RsaI          992  GTAC                 GT^AC
SalI          313  GTCGAC               G^TCGAC
SfaNI         696  GATGC                GCATC(5/9)
SmlI          987  CTCAAG               C^T[CT][GA]AG
StuI          773  AGGCCT               AGG^CCT
XbaI         1260  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          313  GTCGAC               GT^[AC][GT]AC
             1332  GTCTAC              
AciI         1216  CCGC                 CCGC(-3/-1)
              888  GCGG                
AcyI          733  GGCGTC               G[GA]^CG[CT]C
             1227  GACGCC              
BalI          291  TGGCCA               TGG^CCA
              743  TGGCCA              
BbvCI         142  GCTGAGG              CCTCAGC(-5/-2)
             1288  GCTGAGG             
BmyI          476  GGGCAC               G[AGT]GC[ACT]^C
             1254  GTGCCC              
BsaHI         733  GGCGTC               G[GA]^CG[CT]C
             1227  GACGCC              
BseRI         352  GAGGAG               GAGGAG(10/8)
             1204  GAGGAG              
BsmAI        1120  GAGAC                GTCTC(1/5)
             1036  GTCTC               
BsmFI          42  GTCCC                GGGAC(10/14)
              908  GTCCC               
Bsp1286I      476  GGGCAC               G[AGT]GC[ACT]^C
             1254  GTGCCC              
BsrI          160  CCAGT                ACTGG(1/-1)
              883  CCAGT               
EaeI          291  TGGCCA               [CT]^GGCC[GA]
              743  TGGCCA              
HgaI         1227  GACGC                GACGC(5/10)
              734  GCGTC               
Hin1I         733  GGCGTC               G[GA]^CG[CT]C
             1227  GACGCC              
HincII        313  GTCGAC               GT[CT]^[GA]AC
              541  GTCAAC              
HindII        313  GTCGAC               GT[CT]^[GA]AC
              541  GTCAAC              
MluNI         291  TGGCCA               TGG^CCA
              743  TGGCCA              
MscI          291  TGGCCA               TGG^CCA
              743  TGGCCA              
MseI          166  TTAA                 T^TAA
              554  TTAA                
PpuMI         436  GGGTCCT              [GA]G^G[AT]CC[CT]
             1208  AGGACCC             
SbfI           12  CCTGCAGG             CCTGCA^GG
              468  CCTGCAGG            
Sse8387I       12  CCTGCAGG             CCTGCA^GG
              468  CCTGCAGG            
TfiI          464  GAATC                G^A[AT]TC
              646  GATTC               
Tru9I         166  TTAA                 T^TAA
              554  TTAA                
Tsp509I       558  AATT                 ^AATT
             1157  AATT                
XcmI         1136  CCAGGGAGCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1232  CCATGAACAGCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AluI        AlwNI       AspI        AvaII       BbsI        
BbvI        BfaI        BglII       BpuAI       BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       DraII       
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PflFI       PleI        PstI        Sau3AI      Sau96I      
ScrFI       SfcI        TaqI        TseI        Tsp45I      
TspRI       Tth111I     TthHB8I     XhoII       
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