Restriction map for pF1KB0949
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BbvCI       BcgI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AfaI           33  GTAC                 GT^AC
AspHI         249  GAGCTC               G[AT]GC[AT]^C
BanII         249  GAGCTC               G[GA]GC[CT]^C
BbsI          462  GAAGAC               GAAGAC(2/6)
BciVI         431  GGATAC               GTATCC(6/5)
BclI          293  TGATCA               T^GATCA
BfaI          524  CTAG                 C^TAG
BglI           47  GCCTCGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          249  GAGCTC               G[AGT]GC[ACT]^C
BpmI           36  CTGGAG               CTGGAG(16/14)
BpuAI         462  GAAGAC               GAAGAC(2/6)
BsgI          254  CTGCAC               GTGCAG(16/14)
BsiHKAI       249  GAGCTC               G[AT]GC[AT]^C
BsmAI         163  GTCTC                GTCTC(1/5)
BsmBI         162  CGTCTC               CGTCTC(1/5)
Bsp1286I      249  GAGCTC               G[AGT]GC[ACT]^C
BsrBI         415  GAGCGG               CCGCTC(-3/-3)
BsrDI         336  CATTGC               GCAATG(2/0)
BsrFI         228  ACCGGC               [GA]^CCGG[CT]
BstF5I         78  CATCC                GGATG(2/0)
BstNI         330  CCTGG                CC^[AT]GG
Cfr10I        228  ACCGGC               [GA]^CCGG[CT]
DdeI          122  CTCAG                C^T[ACGT]AG
DpnII         294  GATC                 ^GATC
DraI          502  TTTAAA               TTT^AAA
DsaI           18  CCGCGG               C^C[GA][CT]GG
EaeI           86  TGGCCG               [CT]^GGCC[GA]
EcoRII        330  CCTGG                ^CC[AT]GG
FbaI          293  TGATCA               T^GATCA
FokI           78  CATCC                GGATG(9/13)
KspI           18  CCGCGG               CCGC^GG
MaeI          524  CTAG                 C^TAG
MaeII         161  ACGT                 A^CGT
MaeIII        211  GTTAC                ^GT[ACGT]AC
MboI          294  GATC                 ^GATC
MseI          503  TTAA                 T^TAA
MspA1I         18  CCGCGG               C[AC]G^C[GT]G
MvaI          330  CCTGG                CC^[AT]GG
NdeII         294  GATC                 ^GATC
PstI          360  CTGCAG               CTGCA^G
RsaI           33  GTAC                 GT^AC
SacI          249  GAGCTC               GAGCT^C
SacII          18  CCGCGG               CCGC^GG
Sau3AI        294  GATC                 ^GATC
SfcI          360  CTGCAG               C^T[GA][CT]AG
Tru9I         503  TTAA                 T^TAA
Tsp509I       245  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          111  AGCT                 AG^CT
              250  AGCT                
BanI            3  GGCGCC               G^G[CT][GA]CC
               55  GGCGCC              
BbeI            3  GGCGCC               GGCGC^C
               55  GGCGCC              
BsrI           35  ACTGG                ACTGG(1/-1)
              208  CCAGT               
Cfr13I         15  GGCCC                G^G[ACGT]CC
               22  GGGCC               
EarI          145  GAAGAG               CTCTTC(1/4)
              369  GAAGAG              
HgaI           27  GACGC                GACGC(5/10)
              218  GCGTC               
HphI          276  GGTGA                GGTGA(8/7)
              299  TCACC               
KasI            3  GGCGCC               G^GCGCC
               55  GGCGCC              
Ksp632I       145  GAAGAG               CTCTTC(1/4)
              369  GAAGAG              
MslI           78  CATCCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              297  CATCACCATG          
NarI            3  GGCGCC               GG^CGCC
               55  GGCGCC              
Sau96I         15  GGCCC                G^G[ACGT]CC
               22  GGGCC               
SfoI            3  GGCGCC               GGC^GCC
               55  GGCGCC              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AccII       AciI        AcyI        BbvI        BcnI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
BssKI       BstUI       Cac8I       CfoI        FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HpaII       ItaI        MboII       
MnlI        MspI        MvnI        MwoI        NciI        
NlaIII      NlaIV       ScrFI       TseI        
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