Restriction map for pF1KB5061
 (Linear) (Six-base) MAPSORT of: KIBB5061.seq  Check: 421  from: 1  to: 1416



KIBB5061.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0    1263    1416 
   Size:       1263     153
 
AlwNI CAG_nnn'CTG

Cuts at:      0     796    1200    1416 
   Size:        796     404     216

  Fragments arranged by size: 

                 796     404     216
 
ApoI r'AATT_y

Cuts at:      0       4     377    1416 
   Size:          4     373    1039

  Fragments arranged by size: 

                1039     373       4
 
AvrII C'CTAG_G

Cuts at:      0    1408    1416 
   Size:       1408       8
 
BamHI G'GATC_C

Cuts at:      0    1235    1329    1416 
   Size:       1235      94      87

  Fragments arranged by size: 

                1235      94      87
 
BanI G'GyrC_C

Cuts at:      0     895    1024    1416 
   Size:        895     129     392

  Fragments arranged by size: 

                 895     392     129
 
BglII A'GATC_T

Cuts at:      0     954    1416 
   Size:        954     462
 
Bme1580I G_kGCm'C

Cuts at:      0     680    1416 
   Size:        680     736
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     345     836    1121    1416 
   Size:        345     491     285     295

  Fragments arranged by size: 

                 491     345     295     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     334     388     410    1265    1274    1416 
   Size:        319      15      54      22     855       9     142

  Fragments arranged by size: 

                 855     319     142      54      22      15       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1201    1416 
   Size:       1201     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     525    1416 
   Size:        525     891
 
BsaBI GATnn'nnATC

Cuts at:      0     391    1416 
   Size:        391    1025
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     413    1171    1416 
   Size:        413     758     245

  Fragments arranged by size: 

                 758     413     245
 
BseYI C'CCAG_C

Cuts at:      0     878    1416 
   Size:        878     538
 
BsiHKAI G_wGCw'C

Cuts at:      0      55     995    1416 
   Size:         55     940     421

  Fragments arranged by size: 

                 940     421      55
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     680     995    1416 
   Size:         55     625     315     421

  Fragments arranged by size: 

                 625     421     315      55
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     290    1416 
   Size:        290    1126
 
BstEII G'GTnAC_C

Cuts at:      0     456    1416 
   Size:        456     960
 
BstYI r'GATC_y

Cuts at:      0     184     954    1235    1329    1416 
   Size:        184     770     281      94      87

  Fragments arranged by size: 

                 770     281     184      94      87
 
BtgI C'CryG_G

Cuts at:      0     617     899    1206    1305    1416 
   Size:        617     282     307      99     111

  Fragments arranged by size: 

                 617     307     282     111      99
 
BtsI GCAGTG_nn'

Cuts at:      0    1137    1416 
   Size:       1137     279
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     334     388     410    1265    1274    1416 
   Size:        319      15      54      22     855       9     142

  Fragments arranged by size: 

                 855     319     142      54      22      15       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     428     647    1416 
   Size:        428     219     769

  Fragments arranged by size: 

                 769     428     219
 
EcoO109I rG'GnC_Cy

Cuts at:      0     485    1197    1416 
   Size:        485     712     219

  Fragments arranged by size: 

                 712     485     219
 
EcoRI G'AATT_C

Cuts at:      0     377    1416 
   Size:        377    1039
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     752    1305    1416 
   Size:        752     553     111

  Fragments arranged by size: 

                 752     553     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     822     854    1416 
   Size:        822      32     562

  Fragments arranged by size: 

                 822     562      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     712    1088    1416 
   Size:        201     511     376     328

  Fragments arranged by size: 

                 511     376     328     201
 
MspA1I CmG'CkG

Cuts at:      0     125     358     878    1416 
   Size:        125     233     520     538

  Fragments arranged by size: 

                 538     520     233     125
 
NcoI C'CATG_G

Cuts at:      0     899    1206    1416 
   Size:        899     307     210

  Fragments arranged by size: 

                 899     307     210
 
PpuMI rG'GwC_Cy

Cuts at:      0    1197    1416 
   Size:       1197     219
 
PsiI TTA'TAA

Cuts at:      0     518    1416 
   Size:        518     898
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     836     868    1416 
   Size:        836      32     548

  Fragments arranged by size: 

                 836     548      32
 
PstI C_TGCA'G

Cuts at:      0     287     479    1005    1178    1416 
   Size:        287     192     526     173     238

  Fragments arranged by size: 

                 526     287     238     192     173
 
PvuII CAG'CTG

Cuts at:      0     125     358     878    1416 
   Size:        125     233     520     538

  Fragments arranged by size: 

                 538     520     233     125
 
SfcI C'TryA_G

Cuts at:      0     283     475     636     812    1001    1068    1174    1416 
   Size:        283     192     161     176     189      67     106     242

  Fragments arranged by size: 

                 283     242     192     189     176     161     106      67
            
 
SmlI C'TyrA_G

Cuts at:      0     540    1416 
   Size:        540     876
 
SspI AAT'ATT

Cuts at:      0    1059    1416 
   Size:       1059     357
 
StyI C'CwwG_G

Cuts at:      0     584     899    1206    1408    1416 
   Size:        584     315     307     202       8

  Fragments arranged by size: 

                 584     315     307     202       8
 
TatI w'GTAC_w

Cuts at:      0     852    1416 
   Size:        852     564
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28      60    1416 
   Size:         28      32    1356

  Fragments arranged by size: 

                1356      32      28
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1071    1149    1323    1416 
   Size:       1071      78     174      93

  Fragments arranged by size: 

                1071     174      93      78
 
XmnI GAAnn'nnTTC

Cuts at:      0     735    1416 
   Size:        735     681
 

 Enzymes that do cut:

     AccI    AlwNI     ApoI    AvrII    BamHI     BanI    BglII Bme1580I
     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI   BstEII    BstYI     BtgI     BtsI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    Hin4I     MmeI   MspA1I     NcoI    PpuMI
     PsiI     PsrI     PstI    PvuII     SfcI     SmlI     SspI     StyI
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     ZraI
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