(Circular) (Six-base) MAPSORT of: pF1KB4585.seq Check: 7267 from: 1 to: 4151 pF1KB4585 4151 bp With 182 enzymes: SgfI * April 24, 2009 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 415 415 Size: 4151 AccI GT'mk_AC Cuts at: 1091 1189 1091 Size: 98 4053 Acc65I G'GTAC_C Cuts at: 1167 1167 Size: 4151 AclI AA'CG_TT Cuts at: 3991 3991 Size: 4151 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 916 1976 2408 2761 3423 916 Size: 1060 432 353 662 1644 Fragments arranged by size: 1644 1060 662 432 353 AflIII A'CryG_T Cuts at: 395 1503 3303 3584 395 Size: 1108 1800 281 962 Fragments arranged by size: 1800 1108 962 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 383 383 Size: 4151 AlwNI CAG_nnn'CTG Cuts at: 1456 2894 1456 Size: 1438 2713 ApaLI G'TGCA_C Cuts at: 2989 2989 Size: 4151 ApoI r'AATT_y Cuts at: 1155 3504 3760 1155 Size: 2349 256 1546 Fragments arranged by size: 2349 1546 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4151 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4151 AvaI C'yCGr_G Cuts at: 302 1171 302 Size: 869 3282 BamHI G'GATC_C Cuts at: 1176 1176 Size: 4151 BanI G'GyrC_C Cuts at: 841 911 994 1082 1167 1581 1829 1864 841 Size: 70 83 88 85 414 248 35 3128 Fragments arranged by size: 3128 414 248 88 85 83 70 35 BanII G_rGCy'C Cuts at: 209 223 709 1004 1123 1165 2195 209 Size: 14 486 295 119 42 1030 2165 Fragments arranged by size: 2165 1030 486 295 119 42 14 BbeI G_GCGC'C Cuts at: 1585 1833 1585 Size: 248 3903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 366 400 366 Size: 34 4117 BciVI GTATCCnnnnn_n' Cuts at: 222 2049 3105 4151 222 Size: 1827 1056 1046 222 Fragments arranged by size: 1827 1056 1046 222 BclI T'GATC_A Cuts at: 1671 1671 Size: 4151 BglI GCCn_nnn'nGGC Cuts at: 688 688 Size: 4151 BglII A'GATC_T Cuts at: 1666 3703 1666 Size: 2037 2114 BlpI GC'TnA_GC Cuts at: 1263 1263 Size: 4151 Bme1580I G_kGCm'C Cuts at: 997 1085 1776 1869 2993 997 Size: 88 691 93 1124 2155 Fragments arranged by size: 2155 1124 691 93 88 BmrI ACTGGGnnnn_n' Cuts at: 1363 1444 1779 3923 1363 Size: 81 335 2144 1591 Fragments arranged by size: 2144 1591 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2518 2518 Size: 4151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 223 237 499 553 223 Size: 14 262 54 3821 Fragments arranged by size: 3821 262 54 14 Bpu10I CC'TnA_GC Cuts at: 356 626 356 Size: 270 3881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1327 2206 2920 3218 1327 Size: 879 714 298 2260 Fragments arranged by size: 2260 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 3855 3855 Size: 4151 BsaAI yAC'GTr Cuts at: 1504 1504 Size: 4151 BsaBI GATnn'nnATC Cuts at: 6 1665 3702 6 Size: 1659 2037 455 Fragments arranged by size: 2037 1659 455 BsaHI Gr'CG_yC Cuts at: 622 1582 1830 2532 4028 622 Size: 960 248 702 1496 745 Fragments arranged by size: 1496 960 745 702 248 BsaWI w'CCGG_w Cuts at: 1340 1861 2539 2950 3097 3371 1340 Size: 521 678 411 147 274 2120 Fragments arranged by size: 2120 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 649 1003 1576 2334 2999 3407 649 Size: 354 573 758 665 408 1393 Fragments arranged by size: 1393 758 665 573 408 354 BsiEI CG_ry'CG Cuts at: 104 1693 1739 2969 104 Size: 1589 46 1230 1286 Fragments arranged by size: 1589 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 223 1165 1943 2133 2993 223 Size: 942 778 190 860 1381 Fragments arranged by size: 1381 942 860 778 190 Bsp1286I G_dGCh'C Cuts at: 209 223 709 997 1004 1085 1123 1165 1776 Size: 14 486 288 7 81 38 42 611 Cuts at: 1776 1869 1943 2133 2195 2993 209 Size: 93 74 190 62 798 1367 Fragments arranged by size: 1367 798 611 486 288 190 93 81 74 62 42 38 14 7 BspHI T'CATG_A Cuts at: 590 2583 590 Size: 1993 2158 BspMI ACCTGCnnnn'nnnn_ Cuts at: 415 1201 1415 1717 2098 415 Size: 786 214 302 381 2468 Fragments arranged by size: 2468 786 381 302 214 BsrBI CCG'CTC Cuts at: 50 2443 2497 3981 4146 50 Size: 2393 54 1484 165 55 Fragments arranged by size: 2393 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2063 2063 Size: 4151 BsrFI r'CCGG_y Cuts at: 173 844 2149 2330 173 Size: 671 1305 181 1994 Fragments arranged by size: 1994 1305 671 181 BssSI C'ACGA_G Cuts at: 2422 3130 2422 Size: 708 3443 BstAPI GCAn_nnn'nTGC Cuts at: 1528 1528 Size: 4151 BstBI TT'CG_AA Cuts at: 2512 2512 Size: 4151 BstEII G'GTnAC_C Cuts at: 402 402 Size: 4151 BstXI CCAn_nnnn'nTGG Cuts at: 189 201 399 471 189 Size: 12 198 72 3869 Fragments arranged by size: 3869 198 72 12 BstYI r'GATC_y Cuts at: 1 606 1176 1643 1666 2001 2247 2553 2565 Size: 605 570 467 23 335 246 306 12 Cuts at: 2565 2651 3703 1 Size: 86 1052 449 Fragments arranged by size: 1052 605 570 467 449 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4151 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 166 2101 2282 2445 166 Size: 1935 181 163 1872 Fragments arranged by size: 1935 1872 181 163 BtsI GCAGTG_nn' Cuts at: 987 1394 987 Size: 407 3744 DraI TTT'AAA Cuts at: 1150 1150 Size: 4151 DrdI GACnn_nn'nnGTC Cuts at: 670 1857 3201 670 Size: 1187 1344 1620 Fragments arranged by size: 1620 1344 1187 EaeI y'GGCC_r Cuts at: 191 680 962 1736 1910 2301 2328 3673 191 Size: 489 282 774 174 391 27 1345 669 Fragments arranged by size: 1345 774 669 489 391 282 174 27 EagI C'GGCC_G Cuts at: 1736 1736 Size: 4151 EarI CTCTTCn'nnn_ Cuts at: 746 751 2174 2384 746 Size: 5 1423 210 2513 Fragments arranged by size: 2513 1423 210 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2359 3101 3247 3961 2359 Size: 742 146 714 2549 Fragments arranged by size: 2549 742 714 146 EcoICRI GAG'CTC Cuts at: 221 1163 221 Size: 942 3209 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 223 237 499 553 916 1976 2408 2761 3423 Size: 14 262 54 363 1060 432 353 662 Cuts at: 3423 223 Size: 951 Fragments arranged by size: 1060 951 662 432 363 353 262 54 14 EcoNI CCTnn'n_nnAGG Cuts at: 411 411 Size: 4151 EcoO109I rG'GnC_Cy Cuts at: 690 792 1290 690 Size: 102 498 3551 Fragments arranged by size: 3551 498 102 EcoRI G'AATT_C Cuts at: 1155 1155 Size: 4151 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 4151 FspI TGC'GCA Cuts at: 1932 1932 Size: 4151 HaeII r_GCGC'y Cuts at: 169 1585 1833 3063 3834 169 Size: 1416 248 1230 771 486 Fragments arranged by size: 1416 1230 771 486 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 196 228 1993 2023 2025 2055 196 Size: 32 1765 30 2 30 2292 Fragments arranged by size: 2292 1765 32 30 30 2 HincII GTy'rAC Cuts at: 1190 1190 Size: 4151 KasI G'GCGC_C Cuts at: 1581 1829 1581 Size: 248 3903 KpnI G_GTAC'C Cuts at: 1171 1171 Size: 4151 MluI A'CGCG_T Cuts at: 3584 3584 Size: 4151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2910 3094 2910 Size: 184 3967 MscI TGG'CCA Cuts at: 193 682 964 1912 193 Size: 489 282 948 2432 Fragments arranged by size: 2432 948 489 282 MslI CAynn'nnrTG Cuts at: 2140 2267 2140 Size: 127 4024 MspA1I CmG'CkG Cuts at: 189 367 633 788 933 991 1262 1576 1936 Size: 178 266 155 145 58 271 314 360 Cuts at: 1936 2720 2965 189 Size: 784 245 1375 Fragments arranged by size: 1375 784 360 314 271 266 245 178 155 145 58 NaeI GCC'GGC Cuts at: 175 2332 175 Size: 2157 1994 NarI GG'CG_CC Cuts at: 1582 1830 1582 Size: 248 3903 NcoI C'CATG_G Cuts at: 107 107 Size: 4151 NgoMIV G'CCGG_C Cuts at: 173 2330 173 Size: 2157 1994 NspI r_CATG'y Cuts at: 1204 1704 3307 1204 Size: 500 1603 2048 Fragments arranged by size: 2048 1603 500 PasI CC'CwG_GG Cuts at: 711 711 Size: 4151 PciI A'CATG_T Cuts at: 3303 3303 Size: 4151 PfoI T'CCnGG_A Cuts at: 214 449 214 Size: 235 3916 PmeI GTTT'AAAC Cuts at: 1150 1150 Size: 4151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2629 2597 Size: 32 4119 PpuMI rG'GwC_Cy Cuts at: 792 792 Size: 4151 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1120 1152 1120 Size: 32 4119 PstI C_TGCA'G Cuts at: 1198 1883 1198 Size: 685 3466 PvuI CG_AT'CG Cuts at: 104 104 Size: 4151 PvuII CAG'CTG Cuts at: 367 633 1576 1936 367 Size: 266 943 360 2582 Fragments arranged by size: 2582 943 360 266 RsrII CG'GwC_CG Cuts at: 2346 2346 Size: 4151 SacI G_AGCT'C Cuts at: 223 1165 223 Size: 942 3209 SalI G'TCGA_C Cuts at: 1188 1188 Size: 4151 SanDI GG'GwC_CC Cuts at: 792 792 Size: 4151 SapI GCTCTTCn'nnn_ Cuts at: 2174 2384 2174 Size: 210 3941 SbfI CC_TGCA'GG Cuts at: 1198 1198 Size: 4151 ScaI AGT'ACT Cuts at: 331 3668 331 Size: 3337 814 SexAI A'CCwGG_T Cuts at: 344 344 Size: 4151 SfcI C'TryA_G Cuts at: 33 372 1031 1194 1879 2847 3038 33 Size: 339 659 163 685 968 191 1146 Fragments arranged by size: 1146 968 685 659 339 191 163 SfiI GGCCn_nnn'nGGCC Cuts at: 688 688 Size: 4151 SfoI GGC'GCC Cuts at: 1583 1831 1583 Size: 248 3903 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4151 SmaI CCC'GGG Cuts at: 1173 1173 Size: 4151 SmlI C'TyrA_G Cuts at: 1306 2221 2935 3197 1306 Size: 915 714 262 2260 Fragments arranged by size: 2260 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4151 SphI G_CATG'C Cuts at: 1204 1704 1204 Size: 500 3651 StyI C'CwwG_G Cuts at: 107 815 971 1285 1639 107 Size: 708 156 314 354 2619 Fragments arranged by size: 2619 708 354 314 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 703 1734 1867 2326 2535 703 Size: 1031 133 459 209 2319 Fragments arranged by size: 2319 1031 459 209 133 TatI w'GTAC_w Cuts at: 329 3666 329 Size: 3337 814 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3668 3700 3668 Size: 32 4119 Tth111I GACn'n_nGTC Cuts at: 1948 1948 Size: 4151 XbaI T'CTAG_A Cuts at: 1182 1182 Size: 4151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 528 1646 528 Size: 1118 3033 XmaI C'CCGG_G Cuts at: 1171 1171 Size: 4151 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI PpiI PpuMI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AgeI AhdI AleI AloI ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI SacII SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI