Restriction map for pF1KB6685
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccIII      AclI        AfaI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CfoI        ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HhaI        
HinP1I      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NspI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tru9I       Tsp45I      
XbaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII         610  GACGTC               GACGT^C
AccI         1264  GTCGAC               GT^[AC][GT]AC
AcyI          610  GACGTC               G[GA]^CG[CT]C
AflIII        411  ACGCGT               A^C[GA][CT]GT
AlwNI         112  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          550  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI           57  TGGCCA               TGG^CCA
BcgI         1256  GCAAGGTCGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          940  CTAG                 C^TAG
BlpI          237  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      237  GCTGAGC              GC^T[ACGT]AGC
BsaHI         610  GACGTC               G[GA]^CG[CT]C
BsaI          205  GAGACC               GGTCTC(1/5)
BseRI          22  CTCCTC               GAGGAG(10/8)
BsmBI         815  GAGACG               CGTCTC(1/5)
BspHI         768  TCATGA               T^CATGA
BspMI          17  ACCTGC               ACCTGC(4/8)
BsrFI        1199  ACCGGC               [GA]^CCGG[CT]
BstAPI        366  GCACCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         237  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1199  ACCGGC               [GA]^CCGG[CT]
EcoNI         841  CCTAACCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I         1  ATGCAT               ATGCA^T
FauI          234  CCCGC                CCCGC(4/6)
HgaI          817  GACGC                GACGC(5/10)
Hin1I         610  GACGTC               G[GA]^CG[CT]C
HincII       1264  GTCGAC               GT[CT]^[GA]AC
HindII       1264  GTCGAC               GT[CT]^[GA]AC
MaeI          940  CTAG                 C^TAG
MaeIII         95  GTAAC                ^GT[ACGT]AC
MluI          411  ACGCGT               A^CGCGT
MluNI          57  TGGCCA               TGG^CCA
MscI           57  TGGCCA               TGG^CCA
MslI           76  CATGATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI         1148  TCGCGA               TCG^CGA
NsiI            1  ATGCAT               ATGCA^T
PaeR7I        569  CTCGAG               C^TCGAG
PflFI         550  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         396  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          61  CAGCTG               CAG^CTG
RcaI          768  TCATGA               T^CATGA
SacI          598  GAGCTC               GAGCT^C
SalI         1264  GTCGAC               G^TCGAC
SmaI          210  CCCGGG               CCC^GGG
Tth111I       550  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        396  CCATGGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          569  CTCGAG               C^TCGAG
XmaCI         210  CCCGGG               C^CCGGG
XmaI          210  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         412  CGCG                 CG^CG
             1149  CGCG                
AlwI          300  GATCC                GGATC(4/5)
             1017  GGATC               
Asp700I       660  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              868  GAAGAGGTTC          
AspHI         422  GTGCTC               G[AT]GC[AT]^C
              598  GAGCTC              
AvaI          210  CCCGGG               C^[CT]CG[GA]G
              569  CTCGAG              
AvaII         680  GGACC                G^G[AT]CC
              744  GGACC               
BanI          726  GGTGCC               G^G[CT][GA]CC
             1120  GGCACC              
BanII         132  GGGCTC               G[GA]GC[CT]^C
              598  GAGCTC              
BsgI           64  CTGCAC               GTGCAG(16/14)
              373  CTGCAC              
BsiHKAI       422  GTGCTC               G[AT]GC[AT]^C
              598  GAGCTC              
BsoBI         210  CCCGGG               C^[CT]CG[GA]G
              569  CTCGAG              
BstUI         412  CGCG                 CG^CG
             1149  CGCG                
BstYI         299  AGATCC               [GA]^GATC[CT]
             1017  GGATCT              
DraII         274  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              308  AGGGCCT             
DsaI          396  CCATGG               C^C[GA][CT]GG
              476  CCATGG              
EcoO109I      274  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              308  AGGGCCT             
MflI          299  AGATCC               [GA]^GATC[CT]
             1017  GGATCT              
MspA1I         61  CAGCTG               C[AC]G^C[GT]G
              235  CCGCTG              
MvnI          412  CGCG                 CG^CG
             1149  CGCG                
NcoI          396  CCATGG               C^CATGG
              476  CCATGG              
PleI          879  GACTC                GAGTC(4/5)
              340  GAGTC               
PstI          739  CTGCAG               CTGCA^G
             1130  CTGCAG              
SfcI          739  CTGCAG               C^T[GA][CT]AG
             1130  CTGCAG              
TfiI          114  GATTC                G^A[AT]TC
             1193  GATTC               
XcmI          260  CCATGCTTTCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1097  CCACCTTGGACGTGG     
XhoII         299  AGATCC               [GA]^GATC[CT]
             1017  GGATCT              
XmnI          660  GAAACCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              868  GAAGAGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BcnI        BmyI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TseI        Tsp509I     TspRI       
TthHB8I     
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