Restriction map for pF1KB5687
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# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvaII       AviII       AvrII       BanI        BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BpmI        
BsaAI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cfr10I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeIII      MfeI        MluI        
MroI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp45I      Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccI          314  GTAGAC               GT^[AC][GT]AC
AclI          610  AACGTT               AA^CGTT
AcsI          367  GAATTT               [GA]^AATT[CT]
AcyI          462  GACGCC               G[GA]^CG[CT]C
AfaI          431  GTAC                 GT^AC
ApoI          367  GAATTT               [GA]^AATT[CT]
AspHI         442  GAGCTC               G[AT]GC[AT]^C
AvaI          145  CCCGAG               C^[CT]CG[GA]G
BalI          494  TGGCCA               TGG^CCA
BamHI          48  GGATCC               G^GATCC
BanII         442  GAGCTC               G[GA]GC[CT]^C
BbsI          124  GTCTTC               GAAGAC(2/6)
BfaI          662  CTAG                 C^TAG
BglI          577  GCCACCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          530  GCTCAGC              GC^T[ACGT]AGC
BmyI          442  GAGCTC               G[AGT]GC[ACT]^C
Bpu1102I      530  GCTCAGC              GC^T[ACGT]AGC
BpuAI         124  GTCTTC               GAAGAC(2/6)
BsaBI          49  GATCCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         462  GACGCC               G[GA]^CG[CT]C
BsaI          322  GAGACC               GGTCTC(1/5)
BsgI          720  CTGCAC               GTGCAG(16/14)
BsiHKAI       442  GAGCTC               G[AT]GC[AT]^C
BsmFI         227  GGGAC                GGGAC(10/14)
BsmI          526  GAATGC               GAATGC(1/-1)
BsoBI         145  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      442  GAGCTC               G[AGT]GC[ACT]^C
BstF5I        285  GGATG                GGATG(2/0)
BstYI          48  GGATCC               [GA]^GATC[CT]
Cac8I          16  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         530  GCTCAGC              GC^T[ACGT]AGC
CfoI          112  GCGC                 GCG^C
DdeI          531  CTCAG                C^T[ACGT]AG
DrdI          462  GACGCCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI          285  GGATG                GGATG(9/13)
HhaI          112  GCGC                 GCG^C
Hin1I         462  GACGCC               G[GA]^CG[CT]C
HinP1I        112  GCGC                 G^CGC
HinfI         280  GATTC                G^A[ACGT]TC
HphI          355  GGTGA                GGTGA(8/7)
MaeI          662  CTAG                 C^TAG
MamI           49  GATCCTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           48  GGATCC               [GA]^GATC[CT]
MluNI         494  TGGCCA               TGG^CCA
MscI          494  TGGCCA               TGG^CCA
MseI          614  TTAA                 T^TAA
MslI          723  CACTGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIV          48  GGATCC               GG[ACGT]^[ACGT]CC
Psp1406I      610  AACGTT               AA^CGTT
RsaI          431  GTAC                 GT^AC
SacI          442  GAGCTC               GAGCT^C
SfaNI         286  GATGC                GCATC(5/9)
SfcI          161  CTACAG               C^T[GA][CT]AG
TfiI          280  GATTC                G^A[AT]TC
Tru9I         614  TTAA                 T^TAA
XcmI          383  CCAAAATGCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          48  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI           49  GATCC                GGATC(4/5)
               48  GGATC               
AspI          253  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACAGTGTC           
BsrI          185  CCAGT                ACTGG(1/-1)
              466  CCAGT               
DpnII          49  GATC                 ^GATC
              412  GATC                
EaeI          182  CGGCCA               [CT]^GGCC[GA]
              494  TGGCCA              
EarI           11  GAAGAG               CTCTTC(1/4)
              714  GAAGAG              
EcoT14I       131  CCAAGG               C^C[AT][AT]GG
              581  CCTTGG              
HaeIII        183  GGCC                 GG^CC
              495  GGCC                
HgaI          462  GACGC                GACGC(5/10)
              554  GCGTC               
Ksp632I        11  GAAGAG               CTCTTC(1/4)
              714  GAAGAG              
MboI           49  GATC                 ^GATC
              412  GATC                
MnlI          267  CCTC                 CCTC(7/6)
              339  CCTC                
MwoI          487  GCAAATATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              577  GCCACCTTGGC         
NdeII          49  GATC                 ^GATC
              412  GATC                
NspI          586  GCATGT               [GA]CATG^[CT]
              656  ACATGC              
PflFI         253  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACAGTGTC           
SapI           11  GAAGAGC              GCTCTTC(1/4)
              714  GAAGAGC             
Sau3AI         49  GATC                 ^GATC
              412  GATC                
StyI          131  CCAAGG               C^C[AT][AT]GG
              581  CCTTGG              
TaqI          197  TCGA                 T^CGA
              524  TCGA                
Tth111I       253  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              331  GACAGTGTC           
TthHB8I       197  TCGA                 T^CGA
              524  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BsmAI       BssKI       BstNI       EcoRII      
Fnu4HI      ItaI        MaeII       MboII       MvaI        
NlaIII      ScrFI       TseI        Tsp509I     TspRI       
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