Restriction map for pF1KB5978
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AvaI        AvrII       BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BlnI        BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmI        BsoBI       Bsp1407I    BspEI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MunI        MvnI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspBI       SspI        
SwaI        TfiI        Tru9I       Van91I      XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       1064  GGTACC               G^GTACC
AcsI          796  GAATTT               [GA]^AATT[CT]
AcyI          283  GGCGCC               G[GA]^CG[CT]C
AocI          660  CCTCAGG              CC^T[ACGT]AGG
ApaI          369  GGGCCC               GGGCC^C
ApoI          796  GAATTT               [GA]^AATT[CT]
Asp718I      1064  GGTACC               G^GTACC
AspHI         712  GAGCTC               G[AT]GC[AT]^C
AspI         1186  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1121  TGCGCA               TGC^GCA
BalI            2  TGGCCA               TGG^CCA
BbeI          283  GGCGCC               GGCGC^C
BglII        1174  AGATCT               A^GATCT
BlpI         1234  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1234  GCTGAGC              GC^T[ACGT]AGC
BsaHI         283  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       712  GAGCTC               G[AT]GC[AT]^C
BspDI         454  ATCGAT               AT^CGAT
BsrDI        1224  CATTGC               GCAATG(2/0)
BstEII        411  GGTCACC              G^GT[ACGT]ACC
BstPI         411  GGTCACC              G^GT[ACGT]ACC
Bsu36I        660  CCTCAGG              CC^T[ACGT]AGG
CelII        1234  GCTGAGC              GC^T[ACGT]AGC
ClaI          454  ATCGAT               AT^CGAT
DrdI          676  GACAGCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          962  CCATGG               C^C[GA][CT]GG
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EarI         1677  GAAGAG               CTCTTC(1/4)
Eco81I        660  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       411  GGTCACC              G^GT[ACGT]ACC
FspI         1121  TGCGCA               TGC^GCA
HaeII         283  GGCGCC               [GA]GCGC^[CT]
HgaI          104  GCGTC                GACGC(5/10)
Hin1I         283  GGCGCC               G[GA]^CG[CT]C
KasI          283  GGCGCC               G^GCGCC
KpnI         1064  GGTACC               GGTAC^C
Ksp632I      1677  GAAGAG               CTCTTC(1/4)
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
NarI          283  GGCGCC               GG^CGCC
NcoI          962  CCATGG               C^CATGG
PflFI        1186  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI          712  GAGCTC               GAGCT^C
SapI         1677  GAAGAGC              GCTCTTC(1/4)
SbfI         1212  CCTGCAGG             CCTGCA^GG
SexAI         731  ACCTGGT              A^CC[AT]GGT
SfoI          283  GGCGCC               GGC^GCC
SmlI         1258  CTCAAG               C^T[CT][GA]AG
SphI          722  GCATGC               GCATG^C
Sse8387I     1212  CCTGCAGG             CCTGCA^GG
StuI         1521  AGGCCT               AGG^CCT
Tsp509I       797  AATT                 ^AATT
Tth111I      1186  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BglI           86  GCCGCTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              188  GCCTGGGGGGC         
BsgI         1419  GTGCAG               GTGCAG(16/14)
             1465  GTGCAG              
BsmBI         105  CGTCTC               CGTCTC(1/5)
             1476  CGTCTC              
BsrBI          87  CCGCTC               CCGCTC(-3/-3)
              117  CCGCTC              
BsrFI         316  GCCGGC               [GA]^CCGG[CT]
              346  GCCGGC              
BsrI         1481  CCAGT                ACTGG(1/-1)
             1499  CCAGT               
BstYI         206  GGATCT               [GA]^GATC[CT]
             1174  AGATCT              
Cfr10I        316  GCCGGC               [GA]^CCGG[CT]
              346  GCCGGC              
EcoT14I       857  CCAAGG               C^C[AT][AT]GG
              962  CCATGG              
HincII        418  GTCAAC               GT[CT]^[GA]AC
             1450  GTCAAC              
HindII        418  GTCAAC               GT[CT]^[GA]AC
             1450  GTCAAC              
HphI         1411  GGTGA                GGTGA(8/7)
              413  TCACC               
MaeII        1190  ACGT                 A^CGT
             1376  ACGT                
MboII         810  GAAGA                GAAGA(8/7)
             1677  GAAGA               
MflI          206  GGATCT               [GA]^GATC[CT]
             1174  AGATCT              
MslI          945  CACATCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1455  CATCTCTGTG          
NaeI          316  GCCGGC               GCC^GGC
              346  GCCGGC              
NgoMIV        316  GCCGGC               G^CCGGC
              346  GCCGGC              
NspI          148  GCATGT               [GA]CATG^[CT]
              722  GCATGC              
PpuMI         728  AGGACCT              [GA]G^G[AT]CC[CT]
             1316  AGGACCT             
PvuII         225  CAGCTG               CAG^CTG
              670  CAGCTG              
StyI          857  CCAAGG               C^C[AT][AT]GG
              962  CCATGG              
XcmI         1268  CCAAGAACAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1310  CCAAGCAGGACCTGG     
XhoII         206  GGATCT               [GA]^GATC[CT]
             1174  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanI        BanII       BbvCI       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeIII      MboI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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