Restriction map for pF1KB5978
 (Linear) (Six-base) MAPSORT of: KIBB5978.seq  Check: 9092  from: 1  to: 1692



KIBB5978.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

Acc65I G'GTAC_C

Cuts at:      0    1064    1692 
   Size:       1064     628
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     829    1354    1563    1692 
   Size:         81     748     525     209     129

  Fragments arranged by size: 

                 748     525     209     129      81
 
AleI CACnn'nnGTG

Cuts at:      0     949    1692 
   Size:        949     743
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1157    1692 
   Size:       1157     535
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     622     654    1692 
   Size:        622      32    1038

  Fragments arranged by size: 

                1038     622      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     627     732    1170    1320    1350    1692 
   Size:        627     105     438     150      30     342

  Fragments arranged by size: 

                 627     438     342     150     105      30
 
ApaI G_GGCC'C

Cuts at:      0     373    1692 
   Size:        373    1319
 
ApoI r'AATT_y

Cuts at:      0     796    1692 
   Size:        796     896
 
BanI G'GyrC_C

Cuts at:      0     283     604    1064    1495    1692 
   Size:        283     321     460     431     197

  Fragments arranged by size: 

                 460     431     321     283     197
 
BanII G_rGCy'C

Cuts at:      0     199     373     716    1692 
   Size:        199     174     343     976

  Fragments arranged by size: 

                 976     343     199     174
 
BbeI G_GCGC'C

Cuts at:      0     287    1692 
   Size:        287    1405
 
BbvCI CC'TCA_GC

Cuts at:      0     482    1004    1049    1615    1692 
   Size:        482     522      45     566      77

  Fragments arranged by size: 

                 566     522     482      77      45
 
BglI GCCn_nnn'nGGC

Cuts at:      0      92     194    1692 
   Size:         92     102    1498

  Fragments arranged by size: 

                1498     102      92
 
BglII A'GATC_T

Cuts at:      0    1174    1692 
   Size:       1174     518
 
BlpI GC'TnA_GC

Cuts at:      0    1235    1692 
   Size:       1235     457
 
Bme1580I G_kGCm'C

Cuts at:      0     373     391     607    1692 
   Size:        373      18     216    1085

  Fragments arranged by size: 

                1085     373     216      18
 
BmgBI CAC'GTC

Cuts at:      0    1191    1377    1692 
   Size:       1191     186     315

  Fragments arranged by size: 

                1191     315     186
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     105     416     571     649    1309    1426    1464    1653 
   Size:        105     311     155      78     660     117      38     189

Cuts at:   1653    1692 
   Size:         39

  Fragments arranged by size: 

                 660     311     189     155     117     105      78      39
                  38
 
Bpu10I CC'TnA_GC

Cuts at:      0     482    1004    1049    1518    1615    1692 
   Size:        482     522      45     469      97      77

  Fragments arranged by size: 

                 522     482     469      97      77      45
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1243    1692 
   Size:       1243     449
 
BsaHI Gr'CG_yC

Cuts at:      0     284    1692 
   Size:        284    1408
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     622     652    1692 
   Size:        622      30    1040

  Fragments arranged by size: 

                1040     622      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     170     499    1039    1096    1429    1653    1656    1692 
   Size:        170     329     540      57     333     224       3      36

  Fragments arranged by size: 

                 540     333     329     224     170      57      36       3
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1440    1486    1692 
   Size:       1440      46     206

  Fragments arranged by size: 

                1440     206      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     716    1692 
   Size:        716     976
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     111    1482    1692 
   Size:        111    1371     210

  Fragments arranged by size: 

                1371     210     111
 
Bsp1286I G_dGCh'C

Cuts at:      0     199     373     391     607     716    1692 
   Size:        199     174      18     216     109     976

  Fragments arranged by size: 

                 976     216     199     174     109      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     455    1080    1127    1220    1692 
   Size:        455     625      47      93     472

  Fragments arranged by size: 

                 625     472     455      93      47
 
BsrBI CCG'CTC

Cuts at:      0      89     119    1692 
   Size:         89      30    1573

  Fragments arranged by size: 

                1573      89      30
 
BsrDI GCAATG_nn'

Cuts at:      0    1223    1692 
   Size:       1223     469
 
BsrFI r'CCGG_y

Cuts at:      0     316     346    1692 
   Size:        316      30    1346

  Fragments arranged by size: 

                1346     316      30
 
BstEII G'GTnAC_C

Cuts at:      0     411    1692 
   Size:        411    1281
 
BstYI r'GATC_y

Cuts at:      0     206    1174    1692 
   Size:        206     968     518

  Fragments arranged by size: 

                 968     518     206
 
Bsu36I CC'TnA_GG

Cuts at:      0     661    1692 
   Size:        661    1031
 
BtgI C'CryG_G

Cuts at:      0     962    1692 
   Size:        962     730
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     984    1692 
   Size:        984     708
 
BtsI GCAGTG_nn'

Cuts at:      0     135    1207    1518    1560    1596    1692 
   Size:        135    1072     311      42      36      96

  Fragments arranged by size: 

                1072     311     135      96      42      36
 
ClaI AT'CG_AT

Cuts at:      0     455    1692 
   Size:        455    1237
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     682    1692 
   Size:        682    1010
 
EaeI y'GGCC_r

Cuts at:      0       2    1692 
   Size:          2    1690
 
EarI CTCTTCn'nnn_

Cuts at:      0    1672    1692 
   Size:       1672      20
 
EcoICRI GAG'CTC

Cuts at:      0     714    1692 
   Size:        714     978
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     105     416     571     649     829    1309    1354 
   Size:         81      24     311     155      78     180     480      45

Cuts at:   1354    1426    1464    1563    1653    1692 
   Size:         72      38      99      90      39

  Fragments arranged by size: 

                 480     311     180     155      99      90      81      78
                  72      45      39      38      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     592     736    1303    1671    1692 
   Size:        592     144     567     368      21

  Fragments arranged by size: 

                 592     567     368     144      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     369     370     729    1317    1692 
   Size:        369       1     359     588     375

  Fragments arranged by size: 

                 588     375     369     359       1
 
FspI TGC'GCA

Cuts at:      0    1123    1692 
   Size:       1123     569
 
HaeII r_GCGC'y

Cuts at:      0     287    1692 
   Size:        287    1405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1042    1074    1166    1198    1692 
   Size:       1042      32      92      32     494

  Fragments arranged by size: 

                1042     494      92      32      32
 
HincII GTy'rAC

Cuts at:      0     420    1452    1692 
   Size:        420    1032     240

  Fragments arranged by size: 

                1032     420     240
 
KasI G'GCGC_C

Cuts at:      0     283    1692 
   Size:        283    1409
 
KpnI G_GTAC'C

Cuts at:      0    1068    1692 
   Size:       1068     624
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1422    1548    1605    1692 
   Size:       1422     126      57      87

  Fragments arranged by size: 

                1422     126      87      57
 
MscI TGG'CCA

Cuts at:      0       4    1692 
   Size:          4    1688
 
MslI CAynn'nnrTG

Cuts at:      0     949    1459    1692 
   Size:        949     510     233

  Fragments arranged by size: 

                 949     510     233
 
MspA1I CmG'CkG

Cuts at:      0     227     471     672    1692 
   Size:        227     244     201    1020

  Fragments arranged by size: 

                1020     244     227     201
 
NaeI GCC'GGC

Cuts at:      0     318     348    1692 
   Size:        318      30    1344

  Fragments arranged by size: 

                1344     318      30
 
NarI GG'CG_CC

Cuts at:      0     284    1692 
   Size:        284    1408
 
NcoI C'CATG_G

Cuts at:      0     962    1692 
   Size:        962     730
 
NgoMIV G'CCGG_C

Cuts at:      0     316     346    1692 
   Size:        316      30    1346

  Fragments arranged by size: 

                1346     316      30
 
NspI r_CATG'y

Cuts at:      0     152     726    1692 
   Size:        152     574     966

  Fragments arranged by size: 

                 966     574     152
 
PasI CC'CwG_GG

Cuts at:      0      76    1692 
   Size:         76    1616
 
PfoI T'CCnGG_A

Cuts at:      0     548    1692 
   Size:        548    1144
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     622     654    1692 
   Size:        622      32    1038

  Fragments arranged by size: 

                1038     622      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     729    1317    1692 
   Size:        729     588     375

  Fragments arranged by size: 

                 729     588     375
 
PspOMI G'GGCC_C

Cuts at:      0     369    1692 
   Size:        369    1323
 
PstI C_TGCA'G

Cuts at:      0     596    1091    1217    1304    1692 
   Size:        596     495     126      87     388

  Fragments arranged by size: 

                 596     495     388     126      87
 
PvuII CAG'CTG

Cuts at:      0     227     672    1692 
   Size:        227     445    1020

  Fragments arranged by size: 

                1020     445     227
 
SacI G_AGCT'C

Cuts at:      0     716    1692 
   Size:        716     976
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1672    1692 
   Size:       1672      20
 
SbfI CC_TGCA'GG

Cuts at:      0    1217    1692 
   Size:       1217     475
 
SexAI A'CCwGG_T

Cuts at:      0     731    1692 
   Size:        731     961
 
SfcI C'TryA_G

Cuts at:      0     592    1087    1213    1300    1692 
   Size:        592     495     126      87     392

  Fragments arranged by size: 

                 592     495     392     126      87
 
SfoI GGC'GCC

Cuts at:      0     285    1692 
   Size:        285    1407
 
SmlI C'TyrA_G

Cuts at:      0    1258    1692 
   Size:       1258     434
 
SphI G_CATG'C

Cuts at:      0     726    1692 
   Size:        726     966
 
StuI AGG'CCT

Cuts at:      0    1523    1692 
   Size:       1523     169
 
StyI C'CwwG_G

Cuts at:      0     857     962    1692 
   Size:        857     105     730

  Fragments arranged by size: 

                 857     730     105
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     130     325    1516    1692 
   Size:        130     195    1191     176

  Fragments arranged by size: 

                1191     195     176     130
 
TatI w'GTAC_w

Cuts at:      0     647    1649    1692 
   Size:        647    1002      43

  Fragments arranged by size: 

                1002     647      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     565     597    1692 
   Size:        565      32    1095

  Fragments arranged by size: 

                1095     565      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1189    1692 
   Size:       1189     503
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1275    1317    1692 
   Size:       1275      42     375

  Fragments arranged by size: 

                1275     375      42
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     BanI    BanII     BbeI    BbvCI     BglI    BglII     BlpI Bme1580I
    BmgBI     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI     BsgI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstEII
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PfoI     PpiI    PpuMI
   PspOMI     PstI    PvuII     SacI     SapI     SbfI    SexAI     SfcI
     SfoI     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I    CspCI     DraI   DraIII     EagI     EciI    EcoRI
    EcoRV     FalI     FseI    FspAI  HindIII     HpaI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     XmaI
     XmnI     ZraI
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