Restriction map for pF1KB5684
 (Linear) (Six-base) MAPSORT of: KIBB5684.seq  Check: 3194  from: 1  to: 1686



KIBB5684.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     295     901    1686 
   Size:        295     606     785

  Fragments arranged by size: 

                 785     606     295
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    1408    1686 
   Size:        862     546     278

  Fragments arranged by size: 

                 862     546     278
 
AlwNI CAG_nnn'CTG

Cuts at:      0     244     300     641     774    1046    1424    1686 
   Size:        244      56     341     133     272     378     262

  Fragments arranged by size: 

                 378     341     272     262     244     133      56
 
ApaI G_GGCC'C

Cuts at:      0    1502    1686 
   Size:       1502     184
 
ApaLI G'TGCA_C

Cuts at:      0     441    1686 
   Size:        441    1245
 
ApoI r'AATT_y

Cuts at:      0     717    1156    1189    1686 
   Size:        717     439      33     497

  Fragments arranged by size: 

                 717     497     439      33
 
AvaI C'yCGr_G

Cuts at:      0    1626    1686 
   Size:       1626      60
 
BanI G'GyrC_C

Cuts at:      0     283     433     697     830    1686 
   Size:        283     150     264     133     856

  Fragments arranged by size: 

                 856     283     264     150     133
 
BanII G_rGCy'C

Cuts at:      0      23     944    1337    1502    1686 
   Size:         23     921     393     165     184

  Fragments arranged by size: 

                 921     393     184     165      23
 
BbeI G_GCGC'C

Cuts at:      0     437    1686 
   Size:        437    1249
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      45    1686 
   Size:         45    1641
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322     356     679     713    1686 
   Size:        322      34     323      34     973

  Fragments arranged by size: 

                 973     323     322      34      34
 
BglII A'GATC_T

Cuts at:      0     103    1686 
   Size:        103    1583
 
Bme1580I G_kGCm'C

Cuts at:      0     228     286     331     412     445     768     881    1260 
   Size:        228      58      45      81      33     323     113     379

Cuts at:   1260    1502    1686 
   Size:        242     184

  Fragments arranged by size: 

                 379     323     242     228     184     113      81      58
                  45      33
 
BmgBI CAC'GTC

Cuts at:      0     860    1686 
   Size:        860     826
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     755     784    1686 
   Size:        755      29     902

  Fragments arranged by size: 

                 902     755      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1499    1686 
   Size:       1499     187
 
BsaAI yAC'GTr

Cuts at:      0     377     413     837    1686 
   Size:        377      36     424     849

  Fragments arranged by size: 

                 849     424     377      36
 
BsaHI Gr'CG_yC

Cuts at:      0     434    1520    1686 
   Size:        434    1086     166

  Fragments arranged by size: 

                1086     434     166
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     943     973    1686 
   Size:        943      30     713

  Fragments arranged by size: 

                 943     713      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110    1159    1162    1686 
   Size:        110    1049       3     524

  Fragments arranged by size: 

                1049     524     110       3
 
BseYI C'CCAG_C

Cuts at:      0      64     343     510    1595    1686 
   Size:         64     279     167    1085      91

  Fragments arranged by size: 

                1085     279     167      91      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1410    1686 
   Size:       1410     276
 
BsiEI CG_ry'CG

Cuts at:      0    1684    1686 
   Size:       1684       2
 
BsiHKAI G_wGCw'C

Cuts at:      0     182     421     445    1337    1686 
   Size:        182     239      24     892     349

  Fragments arranged by size: 

                 892     349     239     182      24
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     182     228     286     331     412     421     445 
   Size:         23     159      46      58      45      81       9      24

Cuts at:    445     768     881     944    1260    1337    1502    1686 
   Size:        323     113      63     316      77     165     184

  Fragments arranged by size: 

                 323     316     184     165     159     113      81      77
                  63      58      46      45      24      23       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     295     901    1313    1686 
   Size:        295     606     412     373

  Fragments arranged by size: 

                 606     412     373     295
 
BsrBI CCG'CTC

Cuts at:      0    1014    1383    1493    1686 
   Size:       1014     369     110     193

  Fragments arranged by size: 

                1014     369     193     110
 
BsrDI GCAATG_nn'

Cuts at:      0      14    1217    1686 
   Size:         14    1203     469

  Fragments arranged by size: 

                1203     469      14
 
BsrGI T'GTAC_A

Cuts at:      0    1634    1686 
   Size:       1634      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1286    1686 
   Size:       1286     400
 
BstEII G'GTnAC_C

Cuts at:      0    1644    1686 
   Size:       1644      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     510    1686 
   Size:        510    1176
 
BstYI r'GATC_y

Cuts at:      0     103    1686 
   Size:        103    1583
 
BtgI C'CryG_G

Cuts at:      0     669     712    1686 
   Size:        669      43     974

  Fragments arranged by size: 

                 974     669      43
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     939    1686 
   Size:        939     747
 
BtsI GCAGTG_nn'

Cuts at:      0    1068    1686 
   Size:       1068     618
 
DraIII CAC_nnn'GTG

Cuts at:      0     240    1286    1686 
   Size:        240    1046     400

  Fragments arranged by size: 

                1046     400     240
 
EaeI y'GGCC_r

Cuts at:      0    1566    1686 
   Size:       1566     120
 
EarI CTCTTCn'nnn_

Cuts at:      0       7      87     949    1686 
   Size:          7      80     862     737

  Fragments arranged by size: 

                 862     737      80       7
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     504    1079    1315    1686 
   Size:        504     575     236     371

  Fragments arranged by size: 

                 575     504     371     236
 
EcoICRI GAG'CTC

Cuts at:      0    1335    1686 
   Size:       1335     351
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    1408    1499    1686 
   Size:        862     546      91     187

  Fragments arranged by size: 

                 862     546     187      91
 
EcoO109I rG'GnC_Cy

Cuts at:      0     297    1499    1543    1599    1686 
   Size:        297    1202      44      56      87

  Fragments arranged by size: 

                1202     297      87      56      44
 
EcoRI G'AATT_C

Cuts at:      0     717    1189    1686 
   Size:        717     472     497

  Fragments arranged by size: 

                 717     497     472
 
HaeII r_GCGC'y

Cuts at:      0     437    1686 
   Size:        437    1249
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570     865     897     943     975    1348    1380 
   Size:        538      32     295      32      46      32     373      32

Cuts at:   1380    1686 
   Size:        306

  Fragments arranged by size: 

                 538     373     306     295      46      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     433    1686 
   Size:        433    1253
 
MspA1I CmG'CkG

Cuts at:      0     424     485    1233    1253    1311    1424    1595    1686 
   Size:        424      61     748      20      58     113     171      91

  Fragments arranged by size: 

                 748     424     171     113      91      61      58      20
            
 
NarI GG'CG_CC

Cuts at:      0     434    1686 
   Size:        434    1252
 
NspI r_CATG'y

Cuts at:      0    1470    1680    1686 
   Size:       1470     210       6

  Fragments arranged by size: 

                1470     210       6
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     774     827    1686 
   Size:        774      53     859

  Fragments arranged by size: 

                 859     774      53
 
PfoI T'CCnGG_A

Cuts at:      0    1076    1686 
   Size:       1076     610
 
PmlI CAC'GTG

Cuts at:      0     377     413     837    1686 
   Size:        377      36     424     849

  Fragments arranged by size: 

                 849     424     377      36
 
PspOMI G'GGCC_C

Cuts at:      0    1498    1686 
   Size:       1498     188
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     514     546    1686 
   Size:        514      32    1140

  Fragments arranged by size: 

                1140     514      32
 
PstI C_TGCA'G

Cuts at:      0     124     538     610    1232    1310    1686 
   Size:        124     414      72     622      78     376

  Fragments arranged by size: 

                 622     414     376     124      78      72
 
PvuII CAG'CTG

Cuts at:      0    1233    1311    1595    1686 
   Size:       1233      78     284      91

  Fragments arranged by size: 

                1233     284      91      78
 
SacI G_AGCT'C

Cuts at:      0    1337    1686 
   Size:       1337     349
 
SapI GCTCTTCn'nnn_

Cuts at:      0     949    1686 
   Size:        949     737
 
ScaI AGT'ACT

Cuts at:      0     868    1686 
   Size:        868     818
 
SfcI C'TryA_G

Cuts at:      0     120     402     480     534     606    1228    1306    1686 
   Size:        120     282      78      54      72     622      78     380

  Fragments arranged by size: 

                 622     380     282     120      78      78      72      54
            
 
SfoI GGC'GCC

Cuts at:      0     435    1686 
   Size:        435    1251
 
SmaI CCC'GGG

Cuts at:      0    1628    1686 
   Size:       1628      58
 
SspI AAT'ATT

Cuts at:      0     202    1686 
   Size:        202    1484
 
StuI AGG'CCT

Cuts at:      0    1360    1686 
   Size:       1360     326
 
StyI C'CwwG_G

Cuts at:      0     265    1640    1686 
   Size:        265    1375      46

  Fragments arranged by size: 

                1375     265      46
 
TatI w'GTAC_w

Cuts at:      0     302     590     866     908    1634    1686 
   Size:        302     288     276      42     726      52

  Fragments arranged by size: 

                 726     302     288     276      52      42
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     339     371    1314    1346    1686 
   Size:        339      32     943      32     340

  Fragments arranged by size: 

                 943     340     339      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1626    1686 
   Size:       1626      60
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BcgI    BglII Bme1580I    BmgBI     BmrI
     BpmI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI   BstAPI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     EaeI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I     KasI
   MspA1I     NarI     NspI    PflMI     PfoI     PmlI   PspOMI     PsrI
     PstI    PvuII     SacI     SapI     ScaI     SfcI     SfoI     SmaI
     SspI     StuI     StyI     TatI     TstI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrFI   BssHII    BssSI    BstBI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI     EagI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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