Restriction map for
pF1KB5920
(Circular) (Six-base) MAPSORT of: pF1KB5920.seq Check: 3051 from: 1 to: 4709
pF1KB5920 4709 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 457 929 1096 1535 457
Size: 472 167 439 3631
Fragments arranged by size:
3631 472 439 167
AccI GT'mk_AC
Cuts at: 1460 1747 1460
Size: 287 4422
Acc65I G'GTAC_C
Cuts at: 1725 1725
Size: 4709
AclI AA'CG_TT
Cuts at: 4549 4549
Size: 4709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1569 2534 2966 3713 3981 1569
Size: 965 432 747 268 2297
Fragments arranged by size:
2297 965 747 432 268
AflIII A'CryG_T
Cuts at: 485 953 2061 3165 4142 485
Size: 468 1108 1104 977 1052
Fragments arranged by size:
1108 1104 1052 977 468
AgeI A'CCGG_T
Cuts at: 3097 3097
Size: 4709
AhdI GACnn_n'nnGTC
Cuts at: 591 591
Size: 4709
AleI CACnn'nnGTG
Cuts at: 488 1693 488
Size: 1205 3504
AlwNI CAG_nnn'CTG
Cuts at: 169 2014 3581 169
Size: 1845 1567 1297
Fragments arranged by size:
1845 1567 1297
ApaI G_GGCC'C
Cuts at: 367 367
Size: 4709
ApaLI G'TGCA_C
Cuts at: 3479 3479
Size: 4709
ApoI r'AATT_y
Cuts at: 1713 4062 4318 1713
Size: 2349 256 2104
Fragments arranged by size:
2349 2104 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4709
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
AvaI C'yCGr_G
Cuts at: 358 1271 1729 358
Size: 913 458 3338
Fragments arranged by size:
3338 913 458
BamHI G'GATC_C
Cuts at: 909 1734 909
Size: 825 3884
BanI G'GyrC_C
Cuts at: 469 898 917 1725 2139 2387 2422 469
Size: 429 19 808 414 248 35 2756
Fragments arranged by size:
2756 808 429 414 248 35 19
BanII G_rGCy'C
Cuts at: 329 352 367 513 688 1189 1723 2753 329
Size: 23 15 146 175 501 534 1030 2285
Fragments arranged by size:
2285 1030 534 501 175 146 23 15
BbeI G_GCGC'C
Cuts at: 2143 2391 2143
Size: 248 4461
BbsI GAAGACnn'nnnn_
Cuts at: 158 639 764 1327 158
Size: 481 125 563 3540
Fragments arranged by size:
3540 563 481 125
BbvCI CC'TCA_GC
Cuts at: 1336 1336
Size: 4709
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 903 937 903
Size: 34 4675
BciVI GTATCCnnnnn_n'
Cuts at: 2607 3368 4709 2607
Size: 761 1341 2607
Fragments arranged by size:
2607 1341 761
BclI T'GATC_A
Cuts at: 2229 2229
Size: 4709
BglI GCCn_nnn'nGGC
Cuts at: 875 875
Size: 4709
BglII A'GATC_T
Cuts at: 2224 4261 2224
Size: 2037 2672
BlpI GC'TnA_GC
Cuts at: 1821 1821
Size: 4709
Bme1580I G_kGCm'C
Cuts at: 367 901 2334 2427 3483 367
Size: 534 1433 93 1056 1593
Fragments arranged by size:
1593 1433 1056 534 93
BmrI ACTGGGnnnn_n'
Cuts at: 1921 2002 2337 4481 1921
Size: 81 335 2144 2149
Fragments arranged by size:
2149 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 4709
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 589 705 1315 1332 354
Size: 235 116 610 17 3731
Fragments arranged by size:
3731 610 235 116 17
Bpu10I CC'TnA_GC
Cuts at: 323 1336 323
Size: 1013 3696
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1218 1885 2764 3256 3554 540
Size: 678 667 879 492 298 1695
Fragments arranged by size:
1695 879 678 667 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 523 723 4413 523
Size: 200 3690 819
Fragments arranged by size:
3690 819 200
BsaAI yAC'GTr
Cuts at: 486 930 960 2062 486
Size: 444 30 1102 3133
Fragments arranged by size:
3133 1102 444 30
BsaBI GATnn'nnATC
Cuts at: 6 2223 4260 6
Size: 2217 2037 455
Fragments arranged by size:
2217 2037 455
BsaHI Gr'CG_yC
Cuts at: 2140 2388 3090 4586 2140
Size: 248 702 1496 2263
Fragments arranged by size:
2263 1496 702 248
BsaWI w'CCGG_w
Cuts at: 147 650 1898 2419 3097 3371 3518 3929 147
Size: 503 1248 521 678 274 147 411 927
Fragments arranged by size:
1248 927 678 521 503 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 289 292 1312 1438 1579 1582 232
Size: 57 3 1020 126 141 3 3359
Fragments arranged by size:
3359 1020 141 126 57 3 3
BseYI C'CCAG_C
Cuts at: 162 233 1249 2134 2892 3469 3965 162
Size: 71 1016 885 758 577 496 906
Fragments arranged by size:
1016 906 885 758 577 496 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 628 1147 1394 1671 523
Size: 105 519 247 277 3561
Fragments arranged by size:
3561 519 277 247 105
BsiEI CG_ry'CG
Cuts at: 104 2251 2297 3505 104
Size: 2147 46 1208 1308
Fragments arranged by size:
2147 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 329 513 688 1723 2501 2691 3483 329
Size: 184 175 1035 778 190 792 1555
Fragments arranged by size:
1555 1035 792 778 190 184 175
BsmI GAATG_Cn'
Cuts at: 786 786
Size: 4709
Bsp1286I G_dGCh'C
Cuts at: 329 352 367 513 688 901 1189 1723 2334
Size: 23 15 146 175 213 288 534 611
Cuts at: 2334 2427 2501 2691 2753 3483 329
Size: 93 74 190 62 730 1555
Fragments arranged by size:
1555 730 611 534 288 213 190 175
146 93 74 62 23 15
BspEI T'CCGG_A
Cuts at: 147 650 3929 147
Size: 503 3279 927
Fragments arranged by size:
3279 927 503
BspHI T'CATG_A
Cuts at: 3885 3885
Size: 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 437 457 929 1096 1535 1628 1759 1973 2275
Size: 20 472 167 439 93 131 214 302
Cuts at: 2275 2656 437
Size: 381 2490
Fragments arranged by size:
2490 472 439 381 302 214 167 131
93 20
BsrBI CCG'CTC
Cuts at: 50 573 3001 3055 4539 4704 50
Size: 523 2428 54 1484 165 55
Fragments arranged by size:
2428 1484 523 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1680 2621 1680
Size: 941 3768
BsrFI r'CCGG_y
Cuts at: 256 2707 2888 3097 256
Size: 2451 181 209 1868
Fragments arranged by size:
2451 1868 209 181
BsrGI T'GTAC_A
Cuts at: 425 1133 425
Size: 708 4001
BssSI C'ACGA_G
Cuts at: 589 1362 1630 2980 3338 589
Size: 773 268 1350 358 1960
Fragments arranged by size:
1960 1350 773 358 268
BstAPI GCAn_nnn'nTGC
Cuts at: 469 2086 469
Size: 1617 3092
BstBI TT'CG_AA
Cuts at: 1032 3070 1032
Size: 2038 2671
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4709
BstYI r'GATC_y
Cuts at: 1 117 800 909 1055 1734 2201 2224 2559
Size: 116 683 109 146 679 467 23 335
Cuts at: 2559 2805 3817 3903 3915 4261 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 683 679 467 449 346 335 246
146 116 109 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 215 215
Size: 4709
BtgI C'CryG_G
Cuts at: 107 596 623 1229 1404 1653 107
Size: 489 27 606 175 249 3163
Fragments arranged by size:
3163 606 489 249 175 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 410 2659 2840 3003 410
Size: 2249 181 163 2116
Fragments arranged by size:
2249 2116 181 163
BtsI GCAGTG_nn'
Cuts at: 1071 1952 1071
Size: 881 3828
DraI TTT'AAA
Cuts at: 1708 1708
Size: 4709
DrdI GACnn_nn'nnGTC
Cuts at: 2415 3273 2415
Size: 858 3851
EaeI y'GGCC_r
Cuts at: 1038 1226 1242 1599 2294 2468 2859 2886 4231
Size: 188 16 357 695 174 391 27 1345
Cuts at: 4231 1038
Size: 1516
Fragments arranged by size:
1516 1345 695 391 357 188 174 27
16
EagI C'GGCC_G
Cuts at: 2294 2294
Size: 4709
EarI CTCTTCn'nnn_
Cuts at: 1117 2732 2942 1117
Size: 1615 210 2884
Fragments arranged by size:
2884 1615 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 644 1096 2917 3227 3373 4519 644
Size: 452 1821 310 146 1146 834
Fragments arranged by size:
1821 1146 834 452 310 146
EcoICRI GAG'CTC
Cuts at: 327 511 686 1721 327
Size: 184 175 1035 3315
Fragments arranged by size:
3315 1035 184 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 589 705 1315 1332 1569 2534 2966 3713
Size: 235 116 610 17 237 965 432 747
Cuts at: 3713 3981 354
Size: 268 1082
Fragments arranged by size:
1082 965 747 610 432 268 237 235
116 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1297 1297
Size: 4709
EcoO109I rG'GnC_Cy
Cuts at: 173 230 363 364 440 1848 173
Size: 57 133 1 76 1408 3034
Fragments arranged by size:
3034 1408 133 76 57 1
EcoRI G'AATT_C
Cuts at: 1713 1713
Size: 4709
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2728 2728
Size: 4709
FspI TGC'GCA
Cuts at: 2490 2490
Size: 4709
HaeII r_GCGC'y
Cuts at: 2143 2391 3413 4392 2143
Size: 248 1022 979 2460
Fragments arranged by size:
2460 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 863 895 2551 2581 2583 2613 577
Size: 32 254 32 1656 30 2 30 2673
Fragments arranged by size:
2673 1656 254 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1748 1748
Size: 4709
HindIII A'AGCT_T
Cuts at: 838 1027 838
Size: 189 4520
KasI G'GCGC_C
Cuts at: 2139 2387 2139
Size: 248 4461
KpnI G_GTAC'C
Cuts at: 1729 1729
Size: 4709
MluI A'CGCG_T
Cuts at: 4142 4142
Size: 4709
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 974 3380 3564 974
Size: 2406 184 2119
Fragments arranged by size:
2406 2119 184
MscI TGG'CCA
Cuts at: 1040 1228 1244 1601 2470 1040
Size: 188 16 357 869 3279
Fragments arranged by size:
3279 869 357 188 16
MslI CAynn'nnrTG
Cuts at: 488 1693 2698 2825 488
Size: 1205 1005 127 2372
Fragments arranged by size:
2372 1205 1005 127
MspA1I CmG'CkG
Cuts at: 725 819 1131 1434 1491 1647 1655 1820 2134
Size: 94 312 303 57 156 8 165 314
Cuts at: 2134 2494 3507 3752 725
Size: 360 1013 245 1682
Fragments arranged by size:
1682 1013 360 314 312 303 245 165
156 94 57 8
NaeI GCC'GGC
Cuts at: 2890 2890
Size: 4709
NarI GG'CG_CC
Cuts at: 2140 2388 2140
Size: 248 4461
NcoI C'CATG_G
Cuts at: 107 1229 107
Size: 1122 3587
NgoMIV G'CCGG_C
Cuts at: 2888 2888
Size: 4709
NspI r_CATG'y
Cuts at: 1762 2262 3169 1762
Size: 500 907 3302
Fragments arranged by size:
3302 907 500
PasI CC'CwG_GG
Cuts at: 169 1500 169
Size: 1331 3378
PciI A'CATG_T
Cuts at: 3165 3165
Size: 4709
PflMI CCAn_nnn'nTGG
Cuts at: 169 169
Size: 4709
PfoI T'CCnGG_A
Cuts at: 1478 1478
Size: 4709
PmeI GTTT'AAAC
Cuts at: 1708 1708
Size: 4709
PmlI CAC'GTG
Cuts at: 486 486
Size: 4709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4677
PpuMI rG'GwC_Cy
Cuts at: 173 230 173
Size: 57 4652
PspOMI G'GGCC_C
Cuts at: 363 363
Size: 4709
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 661 693 661
Size: 32 4677
PstI C_TGCA'G
Cuts at: 334 448 818 926 1066 1532 1628 1756 2441
Size: 114 370 108 140 466 96 128 685
Cuts at: 2441 334
Size: 2602
Fragments arranged by size:
2602 685 466 370 140 128 114 108
96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4709
PvuII CAG'CTG
Cuts at: 725 819 1131 1647 2134 2494 725
Size: 94 312 516 487 360 2940
Fragments arranged by size:
2940 516 487 360 312 94
RsrII CG'GwC_CG
Cuts at: 2904 2904
Size: 4709
SacI G_AGCT'C
Cuts at: 329 513 688 1723 329
Size: 184 175 1035 3315
Fragments arranged by size:
3315 1035 184 175
SacII CC_GC'GG
Cuts at: 1656 1656
Size: 4709
SalI G'TCGA_C
Cuts at: 1746 1746
Size: 4709
SanDI GG'GwC_CC
Cuts at: 173 173
Size: 4709
SapI GCTCTTCn'nnn_
Cuts at: 2732 2942 2732
Size: 210 4499
SbfI CC_TGCA'GG
Cuts at: 448 1756 448
Size: 1308 3401
ScaI AGT'ACT
Cuts at: 4226 4226
Size: 4709
SfcI C'TryA_G
Cuts at: 33 330 444 814 922 1062 1528 1624 1752
Size: 297 114 370 108 140 466 96 128
Cuts at: 1752 2437 3430 3621 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 370 297 191 140
128 114 108 96
SfoI GGC'GCC
Cuts at: 2141 2389 2141
Size: 248 4461
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4709
SmaI CCC'GGG
Cuts at: 1273 1731 1273
Size: 458 4251
SmlI C'TyrA_G
Cuts at: 519 1197 1864 2779 3271 3533 519
Size: 678 667 915 492 262 1695
Fragments arranged by size:
1695 915 678 667 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4709
SphI G_CATG'C
Cuts at: 1762 2262 1762
Size: 500 4209
StuI AGG'CCT
Cuts at: 1309 1309
Size: 4709
StyI C'CwwG_G
Cuts at: 107 893 1229 1843 2197 107
Size: 786 336 614 354 2619
Fragments arranged by size:
2619 786 614 354 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1405 2292 2425 2884 3093 1405
Size: 887 133 459 209 3021
Fragments arranged by size:
3021 887 459 209 133
TatI w'GTAC_w
Cuts at: 425 1133 1580 4224 425
Size: 708 447 2644 910
Fragments arranged by size:
2644 910 708 447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 4677
Tth111I GACn'n_nGTC
Cuts at: 2506 2506
Size: 4709
XbaI T'CTAG_A
Cuts at: 1740 1740
Size: 4709
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 957 1438 2204 957
Size: 481 766 3462
Fragments arranged by size:
3462 766 481
XmaI C'CCGG_G
Cuts at: 1271 1729 1271
Size: 458 4251
XmnI GAAnn'nnTTC
Cuts at: 658 658
Size: 4709
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsaXI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI