Restriction map for pF1KB5920
 (Linear) (Six-base) MAPSORT of: KIBB5920.seq  Check: 4768  from: 1  to: 1596



KIBB5920.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     349     821     988    1427    1596 
   Size:        349     472     167     439     169

  Fragments arranged by size: 

                 472     439     349     169     167
 
AccI GT'mk_AC

Cuts at:      0    1352    1596 
   Size:       1352     244
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1461    1596 
   Size:       1461     135
 
AflIII A'CryG_T

Cuts at:      0     377     845    1596 
   Size:        377     468     751

  Fragments arranged by size: 

                 751     468     377
 
AhdI GACnn_n'nnGTC

Cuts at:      0     483    1596 
   Size:        483    1113
 
AleI CACnn'nnGTG

Cuts at:      0     380    1585    1596 
   Size:        380    1205      11

  Fragments arranged by size: 

                1205     380      11
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61    1596 
   Size:         61    1535
 
ApaI G_GGCC'C

Cuts at:      0     259    1596 
   Size:        259    1337
 
AvaI C'yCGr_G

Cuts at:      0     250    1163    1596 
   Size:        250     913     433

  Fragments arranged by size: 

                 913     433     250
 
BamHI G'GATC_C

Cuts at:      0     801    1596 
   Size:        801     795
 
BanI G'GyrC_C

Cuts at:      0     361     790     809    1596 
   Size:        361     429      19     787

  Fragments arranged by size: 

                 787     429     361      19
 
BanII G_rGCy'C

Cuts at:      0     221     244     259     405     580    1081    1596 
   Size:        221      23      15     146     175     501     515

  Fragments arranged by size: 

                 515     501     221     175     146      23      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      50     531     656    1219    1596 
   Size:         50     481     125     563     377

  Fragments arranged by size: 

                 563     481     377     125      50
 
BbvCI CC'TCA_GC

Cuts at:      0    1228    1596 
   Size:       1228     368
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     795     829    1596 
   Size:        795      34     767

  Fragments arranged by size: 

                 795     767      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     767    1596 
   Size:        767     829
 
Bme1580I G_kGCm'C

Cuts at:      0     259     793    1596 
   Size:        259     534     803

  Fragments arranged by size: 

                 803     534     259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     481     597    1207    1224    1596 
   Size:        246     235     116     610      17     372

  Fragments arranged by size: 

                 610     372     246     235     116      17
 
Bpu10I CC'TnA_GC

Cuts at:      0     215    1228    1596 
   Size:        215    1013     368

  Fragments arranged by size: 

                1013     368     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432    1110    1596 
   Size:        432     678     486

  Fragments arranged by size: 

                 678     486     432
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     415     615    1596 
   Size:        415     200     981

  Fragments arranged by size: 

                 981     415     200
 
BsaAI yAC'GTr

Cuts at:      0     378     822     852    1596 
   Size:        378     444      30     744

  Fragments arranged by size: 

                 744     444     378      30
 
BsaWI w'CCGG_w

Cuts at:      0      39     542    1596 
   Size:         39     503    1054

  Fragments arranged by size: 

                1054     503      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     181     184    1204    1330    1471    1474    1596 
   Size:        124      57       3    1020     126     141       3     122

  Fragments arranged by size: 

                1020     141     126     124     122      57       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0      54     125    1141    1596 
   Size:         54      71    1016     455

  Fragments arranged by size: 

                1016     455      71      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     415     520    1039    1286    1563    1596 
   Size:        415     105     519     247     277      33

  Fragments arranged by size: 

                 519     415     277     247     105      33
 
BsiHKAI G_wGCw'C

Cuts at:      0     221     405     580    1596 
   Size:        221     184     175    1016

  Fragments arranged by size: 

                1016     221     184     175
 
BsmI GAATG_Cn'

Cuts at:      0     678    1596 
   Size:        678     918
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     244     259     405     580     793    1081    1596 
   Size:        221      23      15     146     175     213     288     515

  Fragments arranged by size: 

                 515     288     221     213     175     146      23      15
            
 
BspEI T'CCGG_A

Cuts at:      0      39     542    1596 
   Size:         39     503    1054

  Fragments arranged by size: 

                1054     503      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329     349     821     988    1427    1520    1596 
   Size:        329      20     472     167     439      93      76

  Fragments arranged by size: 

                 472     439     329     167      93      76      20
 
BsrBI CCG'CTC

Cuts at:      0     465    1596 
   Size:        465    1131
 
BsrDI GCAATG_nn'

Cuts at:      0    1572    1596 
   Size:       1572      24
 
BsrFI r'CCGG_y

Cuts at:      0     148    1596 
   Size:        148    1448
 
BsrGI T'GTAC_A

Cuts at:      0     317    1025    1596 
   Size:        317     708     571

  Fragments arranged by size: 

                 708     571     317
 
BssSI C'ACGA_G

Cuts at:      0     481    1254    1522    1596 
   Size:        481     773     268      74

  Fragments arranged by size: 

                 773     481     268      74
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     361    1596 
   Size:        361    1235
 
BstBI TT'CG_AA

Cuts at:      0     924    1596 
   Size:        924     672
 
BstYI r'GATC_y

Cuts at:      0       9     692     801     947    1596 
   Size:          9     683     109     146     649

  Fragments arranged by size: 

                 683     649     146     109       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     107    1596 
   Size:        107    1489
 
BtgI C'CryG_G

Cuts at:      0     488     515    1121    1296    1545    1596 
   Size:        488      27     606     175     249      51

  Fragments arranged by size: 

                 606     488     249     175      51      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     302    1596 
   Size:        302    1294
 
BtsI GCAGTG_nn'

Cuts at:      0     963    1596 
   Size:        963     633
 
EaeI y'GGCC_r

Cuts at:      0     930    1118    1134    1491    1596 
   Size:        930     188      16     357     105

  Fragments arranged by size: 

                 930     357     188     105      16
 
EarI CTCTTCn'nnn_

Cuts at:      0    1009    1596 
   Size:       1009     587
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     536     988    1596 
   Size:        536     452     608

  Fragments arranged by size: 

                 608     536     452
 
EcoICRI GAG'CTC

Cuts at:      0     219     403     578    1596 
   Size:        219     184     175    1018

  Fragments arranged by size: 

                1018     219     184     175
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     246     481     597    1207    1224    1461    1596 
   Size:        246     235     116     610      17     237     135

  Fragments arranged by size: 

                 610     246     237     235     135     116      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1189    1596 
   Size:       1189     407
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     122     255     256     332    1596 
   Size:         65      57     133       1      76    1264

  Fragments arranged by size: 

                1264     133      76      65      57       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     469     501     755     787    1596 
   Size:        469      32     254      32     809

  Fragments arranged by size: 

                 809     469     254      32      32
 
HindIII A'AGCT_T

Cuts at:      0     730     919    1596 
   Size:        730     189     677

  Fragments arranged by size: 

                 730     677     189
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     866    1596 
   Size:        866     730
 
MscI TGG'CCA

Cuts at:      0     932    1120    1136    1493    1596 
   Size:        932     188      16     357     103

  Fragments arranged by size: 

                 932     357     188     103      16
 
MslI CAynn'nnrTG

Cuts at:      0     380    1585    1596 
   Size:        380    1205      11

  Fragments arranged by size: 

                1205     380      11
 
MspA1I CmG'CkG

Cuts at:      0     617     711    1023    1326    1383    1539    1547    1596 
   Size:        617      94     312     303      57     156       8      49

  Fragments arranged by size: 

                 617     312     303     156      94      57      49       8
            
 
NcoI C'CATG_G

Cuts at:      0    1121    1596 
   Size:       1121     475
 
PasI CC'CwG_GG

Cuts at:      0      61    1392    1596 
   Size:         61    1331     204

  Fragments arranged by size: 

                1331     204      61
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      61    1596 
   Size:         61    1535
 
PfoI T'CCnGG_A

Cuts at:      0    1370    1596 
   Size:       1370     226
 
PmlI CAC'GTG

Cuts at:      0     378    1596 
   Size:        378    1218
 
PpuMI rG'GwC_Cy

Cuts at:      0      65     122    1596 
   Size:         65      57    1474

  Fragments arranged by size: 

                1474      65      57
 
PspOMI G'GGCC_C

Cuts at:      0     255    1596 
   Size:        255    1341
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     553     585    1596 
   Size:        553      32    1011

  Fragments arranged by size: 

                1011     553      32
 
PstI C_TGCA'G

Cuts at:      0     226     340     710     818     958    1424    1520    1596 
   Size:        226     114     370     108     140     466      96      76

  Fragments arranged by size: 

                 466     370     226     140     114     108      96      76
            
 
PvuII CAG'CTG

Cuts at:      0     617     711    1023    1539    1596 
   Size:        617      94     312     516      57

  Fragments arranged by size: 

                 617     516     312      94      57
 
SacI G_AGCT'C

Cuts at:      0     221     405     580    1596 
   Size:        221     184     175    1016

  Fragments arranged by size: 

                1016     221     184     175
 
SacII CC_GC'GG

Cuts at:      0    1548    1596 
   Size:       1548      48
 
SanDI GG'GwC_CC

Cuts at:      0      65    1596 
   Size:         65    1531
 
SbfI CC_TGCA'GG

Cuts at:      0     340    1596 
   Size:        340    1256
 
SfcI C'TryA_G

Cuts at:      0     222     336     706     814     954    1420    1516    1596 
   Size:        222     114     370     108     140     466      96      80

  Fragments arranged by size: 

                 466     370     222     140     114     108      96      80
            
 
SmaI CCC'GGG

Cuts at:      0    1165    1596 
   Size:       1165     431
 
SmlI C'TyrA_G

Cuts at:      0     411    1089    1596 
   Size:        411     678     507

  Fragments arranged by size: 

                 678     507     411
 
StuI AGG'CCT

Cuts at:      0    1201    1596 
   Size:       1201     395
 
StyI C'CwwG_G

Cuts at:      0     785    1121    1596 
   Size:        785     336     475

  Fragments arranged by size: 

                 785     475     336
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1297    1596 
   Size:       1297     299
 
TatI w'GTAC_w

Cuts at:      0     317    1025    1472    1596 
   Size:        317     708     447     124

  Fragments arranged by size: 

                 708     447     317     124
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     849    1330    1596 
   Size:        849     481     266

  Fragments arranged by size: 

                 849     481     266
 
XmaI C'CCGG_G

Cuts at:      0    1163    1596 
   Size:       1163     433
 
XmnI GAAnn'nnTTC

Cuts at:      0     550    1596 
   Size:        550    1046
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BcgI     BglI
 Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I
     NcoI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PsrI
     PstI    PvuII     SacI    SacII    SanDI     SbfI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI    BsmBI    BspHI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PvuI
    RsrII     SalI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI
  Tth111I     XbaI     XhoI     ZraI
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